Effect of cultivation factors on embryogenesis in isolated microspore culture of carrot (Daucus carota L.)

• Autorzy: Voronina Anastasia V. , Vishnyakova Anastasiia V. , Monakhos Sokrat G. , Monakhos Grigory F. , Ushanov Alexander A. , Mironov Aleksey A.

Identyfikatory

DOI

10.24425/jwld.2022.142314

• Języki publikacji: EN

Abstrakty

EN

Using doubled haploid technologies inbreeding can significantly reduce the time to obtain homozygous parental lines required for the production of F1-hybrid of vegetable crops. This study aims to investigate the influence of factors on the efficiency of carrot embryogenesis in isolated microspore culture to optimise the elements of protocol for producing doubled haploids. Microspores were isolated from inflorescences of 21 genotypes and incubated in NLN13 medium supplemented with 0.1 mg·dm^-3 2,4-dichlorophenoxyacetic acids, 0.1 mg·dm^-3 1-naphthyl acetic acids, 130 g·dm^-3 sucrose, and 400 mg·dm^-3 casein hydrolysate and its modifications. Embryoids and their groups were formed after 2–6 months, in some cases after 12 months of cultivation. Depending on the variant, the embryogenesis efficiency averaged from 0 to 4.9 embryoids or groups of embryoids per Petri dish (10 cm³). Embryoids within the group were formed from different microspores. No significant effects of inflorescence position on the plant (branching order), sucrose, and casein hydrolysate concentration in the medium were observed. Significant advantages (p ≥ 0.05) for some genotypes were shown: 1) microspore suspension density 4·10⁴ cells·cm^-3 (5.0 embryoids per Petri dish were formed at a microspore suspension density of 4·10⁴ cells·cm^-3, 0.0 embryoids per Petri dish at a density of 8·10⁴ cells·cm^-3); 2) cultivating microspores of tetrad and early mononuclear stage (4.9 ±3.1 embryoids per Petri dish were obtained by culturing tetrads and early mononuclear microspores, while 0.6 ±0.7 embryoids per Petri dish were obtained by culturing of later developmental stages); 3) high-temperature treatment duration of five days (4.9 ±2.1 embryoids per Petri dish were obtained after five days of high-temperature treatment, 2.7 ±2.6 embryoids per Petri dish formed after two days of high-temperature treatment; 9.8 ±4.7, 10.1 ±6.1, 0.0 ±0.0 embryoids per Petri dish formed after two, five and eight days of high-temperature treatment respectively); 4) adding colchicine 0.5 mg·dm^-3 to the nutrient medium for two days of high-temperature treatment, followed by medium replacement (3.3 ±2.6 embryoids per Petri dish were obtained by using a nutrient medium with colchicine, while 1.7 ±1.5 embryoids per Petri dish were obtained by culturing in the reference variant).

Słowa kluczowe

EN

carrot; Daucus carota L.; doubled haploids; embryogenesis; isolated microspore culture; male sterility

• Wydawca: Wydawnictwo ITP
• Czasopismo: Journal of Water and Land Development
• Rocznik: 2022
• Tom: no. 55
• Strony: 125--128
• Opis fizyczny: Bibliogr. 14 poz., tab.

Twórcy

autor

Voronina Anastasia V.

• Russian State Agrarian University, Department of Botany, Plant Breeding and Seed Technology, Timiryazevskaya street, 49127550, Moscow, Russian Federation

• https://orcid.org/0000-0003-0249-246X

autor

Vishnyakova Anastasiia V.

• Russian State Agrarian University, Department of Botany, Plant Breeding and Seed Technology, Timiryazevskaya street, 49127550, Moscow, Russian Federation

• https://orcid.org/0000-0002-9160-1164

autor

Monakhos Sokrat G.

• Russian State Agrarian University, Department of Botany, Plant Breeding and Seed Technology, Timiryazevskaya street, 49127550, Moscow, Russian Federation

• https://orcid.org/0000-0001-9404-8862

autor

Monakhos Grigory F.

• Russian State Agrarian University, Breeding Station after N.N. Timofeev, Moscow, Russian Federation

• https://orcid.org/0000-0002-6603-6933

autor

Ushanov Alexander A.

• Russian State Agrarian University, Department of Botany, Plant Breeding and Seed Technology, Timiryazevskaya street, 49127550, Moscow, Russian Federation

• https://orcid.org/0000-0001-6745-9236

autor

Mironov Aleksey A.

• Russian State Agrarian University, Department of Botany, Plant Breeding and Seed Technology, Timiryazevskaya street, 49127550, Moscow, Russian Federation

• https://orcid.org/0000-0002-0297-500X

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).