Simultaneous determination of dantrolene with ibuprofen and diclofenac in plasma by HPLC-DAD : application to comparative pharmacokinetic study

• Autorzy: Abdel Moneim Mona M.

Identyfikatory

DOI

10.1556/1326.2022.01089

• Języki publikacji: EN

Abstrakty

EN

Muscle relaxants and pain killers with their different types are widely used as combination approach for treatment of pain associated with several muscle spasm conditions. A sensitive and simple HPLC-UV detection method was developed in this work for simultaneous assay of Dantrolene (DNT) and co-administrated: Ibuprofen (IBU) and Diclofenac (DIC). After simple protein precipitation, separation was achieved using C₁₈ column (150 × 4.6 mm) with a mobile phase of acidified water with orthophosphoric acid (pH = 3.5) and acetonitrile using gradient elution with a flow rate of 1 mL/min. The DAD was adjusted at 380, 219, 280 and 240 nm to measure DNT, IBU, DIC, and dexamethasone (internal standard), respectively. Linearity was demonstrated over the range from 0.1 to 3 μg/mL, 1 to 40 μg/mL, and 0.1 to 2 μg/mL for DNT, IBU, and DIC, respectively. The validated method was applied successfully to compare the effect of co-administration of IBU or DIC on the pharmacokinetic profile of DNT.

Słowa kluczowe

EN

Dantrolene; Ibuprofen; Diclofenac; HPLC-DAD; pharmacokinetics; plasma

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 1
• Strony: 23--30
• Opis fizyczny: Bibliogr. 24 poz., rys., tab., wykr.

Twórcy

autor

Abdel Moneim Mona M.

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt

• https://orcid.org/0000-0001-8856-7949

Bibliografia

• 1. Ragab, M. A.; Korany, M. A.; Galal, S. M.; Ahmed, A. R. Bioanalysis 2019, 11, 73.
• 2. Krause, T.; Gerbershagen, M.; Fiege, M.; Weisshorn, R.; Wappler, F. Anaesthesia 2004, 59, 364.
• 3. Podranski, T.; Bouillon, T.; Schumacher, P. M.; Taguchi, A.; Sessler, D. I.; Kurz, A. Anesth. Analg. 2005, 101, 1695.
• 4. Sweetman, S. C. Martindale: the Complete Drug Reference, 2005.
• 5. Abdelwahab, N. S.; Abdelrahman, M. M. J. Fluoresc. 2014, 24, 129.
• 6. Luo, X.; Pietrobon, R.; Curtis, L. H.; Hey, L. A. Spine 2004, 29, E531.
• 7. Skeletal muscle relaxant, PrDantrium® intravenous, Dantrolene sodium for injection, USP, Product Monograph, 2018.
• 8. Dantrolene, Drug Bank. https://go.drugbank.com/drugs/DB01219 (accessed Aug 3, 2022).
• 9. El-Bagary, R. I.; Elkady, E. F.; Hegazi, M. A.; Amin, N. E. Eur. J. Chem. 2014, 5, 96.
• 10. Ali, N. W.; Hegazy, M. A.; Abdelkawy, M.; Abdelaleem, E. A. Acta Pharm. 2012, 62, 191.
• 11. Zaazaa, H. E.; Elzanfaly, E. S.; Soudi, A. T.; Salem, M. Y. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2015, 143, 251.
• 12. Safwat, N.; Abdel-Ghany, M. F.; Ayad, M. F. J. AOAC Int. 2021, 104, 103.
• 13. Abdelwahab, N. S.; Elsaady, M. T.; Korany, A. G.; Hgazy, M. A. JPC-J. Planar Chromatogr. 2016, 29, 462.
• 14. Seigel, A.; Schröck, A.; Hauser, R.; Spangenberg, B. J. Liq. Chromatogr. Relat. Technol. 2011, 34, 817.
• 15. Afkhami, A.; Bahiraei, A.; Madrakian, T. Mater. Sci. Eng. 2016, 59, 168.
• 16. Serrano, N.; Castilla, Ò.; Ariño, C.; Diaz-Cruz, M. S.; Díaz-Cruz, J. M. Sensors 2019, 19, 4039.
• 17. Malá, Z.; Gebauer, P.; Boček, P. Anal. Chim. Acta 2016, 907, 1.
• 18. Rizk, M. S.; Sultan, M.; Mohamed, D.; MoussaTony, R. J. Chromatogr. B 2021, 122816.
• 19. Hadad, G. M.; Emara, S.; Mahmoud, W. M. Talanta 2009, 79, 1360.
• 20. Elkady, E. F. Talanta 2010, 82, 1604.
• 21. Ali, N. W.; Hegazy, M. A.; Abdelkawy, M.; Abdelaleem, E. A. J. Liq. Chromatogr. Relat. Technol. 2012, 35, 2229.
• 22. Muntean, D.; Vlase, L.; Cuciureanu, R.; Cuciureanu, M. Rev. Roum. Chim. 2011, 56, 19.
• 23. Chen, T.; Li, Q.; Lu, J.; Yu, C.; Chen, C.; Li, Z. Bioanalysis 2016, 8, 1237.
• 24. Food and Drug Administration, FDA; Guidance for Industry, Bioanalytical Methods Validation, 2001.