LC-MS/MS determination of glimepiride in human plasma with a high recovery at picogram scale : pharmacokinetic study after oral administration

• Autorzy: Kammoun Ahmed K. , Awan Zuhier Ahmed , Elawady Tarek , Khedr Alaa , El-Awady Mohamed I.

Identyfikatory

DOI

10.1556/1326.2020.00841

• Języki publikacji: EN

Abstrakty

EN

Although glimepiride (GLM) is the first-line treatment of Type II diabetes, low extraction recovery is still a significant limitation in previous plasma analysis methods. An optimized solid-phase extraction method of GLM in human plasma with excellent extraction recovery, 100 ± 0.06%, was achieved using liquid chromatography-electrospray ionization tandem mass spectrometry and Gliclazide (GLZ) as an internal standard. GLM was extracted from 100 µL plasma sample using Sep-Pak® vac 1cc (100 mg) C18 column and methylene chloride: methanol (2: 1, v/v) as eluant. Both GLM and GLZ were monitored by a triple quad mass spectrometer applying positive multiple reaction monitoring mode (+MRM). The protonated precursor ions and product ions of GLM and GLZ were m/z 491(352), and m/z 324 (127), respectively. The detection and measurement of low levels of GLM in human plasma reached to picogram range (limit of detection (LOD) = 60 pg/mL, limit of quantification (LOQ) = 200 pg/mL). The method was validated in terms of selectivity, linearity, recovery, accuracy, and precision. The method was successfully applied to the pharmacokinetic study of GLM following oral administration of 1 mg GLM tablets to 12 healthy volunteers.

Słowa kluczowe

EN

glimepiride; plasma; SPE C18 extraction; excellent extraction recovery; pharmacokinetic

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2022
• Tom: Vol. 34, no. 1
• Strony: 12--17
• Opis fizyczny: Bibliogr. 15 poz., rys., tab

Twórcy

autor

Kammoun Ahmed K.

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia

autor

Awan Zuhier Ahmed

• Department of Clinical Biochemistry, Faculty of Medicine, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia

autor

Elawady Tarek

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia

• https://orcid.org/0000-0002-3473-3136

autor

Khedr Alaa

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia

autor

El-Awady Mohamed I.

• Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt

• https://orcid.org/0000-0003-2675-7530

Bibliografia

• 1. McCall, A.L. Expert Opin. Pharmacother. 2001, 2, 699–713.
• 2. Rosenstock, J. ; Samols, E. ; Muchmore, D.B. ; Schneider, J. Diabetes Care 1996, 19, 1194–1199.
• 3. Langtry, H.D. ; Balfour, J.A. Drugs 1998, 55, 563–584.
• 4. Moffat, A.C. ; Osselton, M.D. ; Widdop, B. Clarke's analysis of drugs and poisons, Pharmaceutical Press: London, 2011.
• 5. Ratheiser, K. ; Korn, A. ; Waldhausl, W. ; Komjati, M. ; Vierhapper, H. ; Badian, M. ; Malerczyk, V. Arzneim. Forsch. 1993, 43, 856–858.
• 6. El-Enany, N. M. ; Abdelal, A. A. ; Belal, F. F. ; Itoh, Y. I. ; Nakamura, M. N. Chem. Cent. J. 2012, 6, 9.
• 7. Lakshmi, K. S. ; Rajesh, T. ISRN Pharm. 2011, 2011, 521353.
• 8. Musmade, P. B. ; Talole, K. B. ; Deshpande, P. B. ; Karthik, A. ; Pathak, S. M. ; Pandey, S. ; Udupa, N. Arzneim. Forsch. 2011, 61, 23–31.
• 9. Tripathi, A. S. ; Dewani, A. P. ; Shelke, P. G. ; Bakal, R. L. ; Chandewar, A. V. ; Mazumder, P. M. Drug Res. (Stuttg) 2013, 63, 510–514.
• 10. Kundlik, M. L. ; Zaware, B. H. ; Kuchekar, S. R. J. Chromatogr. Sci. 2012, 50, 64–70.
• 11. Surendran, S. ; Paul, D. ; Sushmita, R. ; Krishna, L. ; Tiwari, N. K. ; Giri, S. ; Satheeshkumar, N. J. Chromatogr. B 2017, 1046, 218–225.
• 12. Strugaru, A.-M. ; Kazakova, J. ; Butnaru, E. ; Caba, I.-C. ; Bello-López, M. Á. ; Fernández-Torres, R. J. Pharm. Biomed. Anal. 2019, 165, 276–283.
• 13. Zhu, J. ; Li, Y. ; Xiang, Y. ; Zhou, L. ; Li, Y. J. Pharm. Biomed. Anal. 2020, 184, 113180.
• 14. U. S. P. Convention , U. S. Pharmacopoeia National Formulary. United States Pharmacopeial, 2012.
• 15. Malerczyk, V. ; Badian, M. ; Korn, A. ; Lehr, K. H. ; Waldhausl, W. Drug Metab. Drug Interact. 1994, 11, 341–357.

Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).