Production of indole acetic acid by endophytic Bacillus strains

• Autorzy: Nabrdalik Małgorzata

Identyfikatory

DOI

10.2428/ecea.2018.25(1)10

Warianty tytułu

PL

Produkcja kwasu indolilooctowego przez endofityczne szczepy Bacillus

• Języki publikacji: EN

Abstrakty

EN

The objectives of this study were to isolate and characterize endophytic bacteria from sugar beet roots focusing on their ability to produce indole acetic acid (IAA). In order to isolate endophytic bacteria the sugar beet roots were used. To determine the amounts of IAA produced by endophytic Bacillus strains (B. amyloliquefaciens, B. megaterium and B. subtilis), a colorimetric technique was applied with Salkowski reagent. The isolates were grown in Laurin Broth medium supplemented with L-tryptophan over the concentration range of 100–10000 g/cm³ and incubated at 30 ^oC for 7 days. The highest concentration of IAA was recorded after 4 days of culturing in the supernatant obtained from the media containing 10000 g/cm³ of tryptophan. For the strain B. subtilis and B. megaterium the concentration of IAA marked in the post –culturing liquid amounted about 82.00 g/cm³, and for the B. amyloliquefaciens strain it amounted over 121.28 g/cm³. The strains Bacillus under study produced IAA in a different amount in the presence of L-tryptophan and in its absence.

PL

Celem pracy było wyizolowanie i scharakteryzowanie bakterii endofitycznych korzeni buraka cukrowego pod kątem ich zdolności do produkcji kwasu indolilooctowego (IAA). Materiał doświadczalny do izolacji bakterii endofitycznych stanowiły korzenie burak cukrowego. Ilość wytworzonego IAA przez endofityczne szczepy Bacillus (B. amyloliquefaciens, B. megaterium, B. subtilis) oznaczono metodą kolorymetryczną z odczynnikiem Salkowskiego. Szczepy hodowano w pożywce LB (Laurin Broth) suplementowanej L-tryptofanem w stężeniach 100–10000 g/cm³ i inkubowano w 30 ^oC przez 7 dni. Najwyższe stężenie IAA odnotowano po 4 dniach w hodowlach zawierających 10000 g/cm³ L-tryptofanu. Dla szczepu B. subtilis i B. megaterium stężenie IAA wynosiło około 82,00 g/cm³, a dla szczepu B. amyloliquefaciens 127,28 g/cm³. Badane szczepy Bacillus spp. wytwarzały IAA na różnym poziomie zarówno w obecności L-tryptofanu jak i przy jego braku.

Słowa kluczowe

EN

Bacillus; endophytes; tryptophan; auxin

PL

Bacillus; endofity; tryptofan; auksyny

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. A
• Rocznik: 2018
• Tom: Vol. 25, nr 1
• Strony: 93--105
• Opis fizyczny: Bibliogr. 29 poz., wykr., tab.

Twórcy

autor

Nabrdalik Małgorzata

• Independent Chair of Biotechnology and Molecular Biology, University of Opole, ul. kard. B. Kominka 6, 45-032 Opole, Poland, phone: [phone]

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).