Development and validation of a method for densitometric analysis of lupeol from Mimosoups elengi

• Autorzy: Ganu G. P , Jadhav S. S , Deshpande A. D
• Języki publikacji: EN

Abstrakty

EN

In this paper we describe a simple, precise, and accurate HPTLC method for quantification of lupeol in the bark of Mimosoups elengi (ME). The bark was extracted with hot methanol in a Soxhlet apparatus. Chromatographic separation of the extract was performed on aluminum foil plates coated with silica gel 60 F 254 as the stationary phase. The mobile phase was toluene-ethyl acetate-formic acid 12:2:1 ( υ/υ ). Densitometric evaluation of the separated zones was performed at 220 nm. The lupeol was satisfactorily resolved at R F 0.64 ± 0.02. The accuracy and reliability of the method were assessed by evaluation of linearity (1000–4000 ng per band), precision (method and instrumental precision, as RSD, 1.06 and 1.03%, respectively), accuracy (97.28 ± 0.89), and specificity in accordance with ICH guidelines.

Słowa kluczowe

EN

HPTLC; validation; lupeol; Mimosoups elengi

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 3
• Strony: 491--497
• Opis fizyczny: Bibliogr. 6 poz., rys., tab.

Twórcy

autor

Ganu G. P

• Pad. Dr D.Y. Patil Institute of Pharmaceutical Sciences and Research Department of Pharmacology Pimpri, Pune India

autor

Jadhav S. S

• Serum Institute of India Pune India

autor

Deshpande A. D

• Pad. Dr D.Y. Patil Institute of Pharmaceutical Sciences and Research Department of Pharmacy Pimpri, Pune India

Bibliografia

• [1] R.N. Chopra, S.L Nayar, and I.C. Chopra, Glossary of Indian Medicinal Plants, National Institute of Science Communication and Information Resources (CSIR), New Delhi, 2000, 167
• [2] P.J. Shah, M.S. Gandhi, M.B. Shah, S.S. Goswami, and D. Santani, J. Ethnopharmacol., 89 , 305 (2003)
• [3] Anonymous, The Wealth of India, Vol. III, Publications and Information Directorate, New Delhi, 1962, 385
• [4] N. Jahan, A. Malik, G. Mustafa, Z. Ahmad, S. Ahmad, E. Anis, S. Malik, S. Shujaat, N. Afza, and A.U. Rahman, Nat. Prod. Lett. 15 , 177 (2001)
• [5] N. Jahan, W. Ahmed, and A. Malik, Phytochemistry, 39 , 255 (1995)
• [6] ICH, Q2B, Harmonised tripartite guideline, text validation of analytical procedures, IFPMA, in: Proceedings of the International Conference on Harmonization, Geneva, 1996