A bioreactor study of the metabolic repertoire and morphology of actinomycete Streptomyces rimosus ATCC 10970 under various initial concentrations of carbon and nitrogen sources

Bizukojć, Marcin; Ścigaczewska, Anna; Boruta, Tomasz; Ruda, Agnieszka; Kawka, Aleksandra

doi:10.24425/cpe.2024.147414

Artykuł - szczegóły

Tytuł artykułu

A bioreactor study of the metabolic repertoire and morphology of actinomycete Streptomyces rimosus ATCC 10970 under various initial concentrations of carbon and nitrogen sources

Autorzy

Bizukojć Marcin , Ścigaczewska Anna , Boruta Tomasz , Ruda Agnieszka , Kawka Aleksandra

Treść / Zawartość

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Identyfikatory

DOI

10.24425/cpe.2024.147414

Warianty tytułu

Języki publikacji

Abstrakty

This work aims at investigating the influence of the initial concentrations of carbon (glucose) and organic nitrogen (yeast extract) sources on Streptomyces rimosus ATCC10970 secondary metabolism in the stirred tank bioreactors. Additionally, glucose utilisation, biomass formation, pH, redox potential and dissolved oxygen levels, and the morphological development of S. rimosus pseudomycelium were studied. Eighteen secondary metabolites were detected by mass spectrometry and identified with the use of the authentic standard, or putatively with the use of literature and database of secondary metabolites. Varied initial yeast extract concentration acted much stronger on the formation of secondary metabolites than glucose did. For example, oxytetracycline was not biosynthesised at high yeast extract concentration while the formation of three other metabolites was enhanced under these conditions. In the case of glucose its increasing initial concentration led to higher secondary metabolite levels with the exception of an unnamed angucycline. High initial yeast extract concentration also drastically changed S. rimosus pseudomycelial morphology from the pelleted to the dispersed one. Ultimately, the cultivation media with the varied initial levels of carbon and nitrogen sources were proved to have the marked effect on S. rimosus secondary metabolism and to be the simplest way to either induce or block the formation of the selected secondary metabolites.

Słowa kluczowe

actinomycete batch bioreactor secondary metabolites Streptomyces rimosus microbial morphology

promieniowce bioreaktor wsadowy metabolity wtórne Streptomyces rimosus morfologia mikroorganizmów

Wydawca

Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk

Czasopismo

Chemical and Process Engineering : New Frontiers

Rocznik

2024

Tom

Vol. 45, nr 2

Strony

art. no. e56

Opis fizyczny

Bibliogr. 50 poz., wykr., tab.

Twórcy

autor

Bizukojć Marcin

Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

https://orcid.org/0000-0003-1641-9917

autor

Ścigaczewska Anna

Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

https://orcid.org/0000-0003-4104-0263

autor

Boruta Tomasz

Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

https://orcid.org/0000-0002-8807-878X

autor

Ruda Agnieszka

Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

autor

Kawka Aleksandra

Lodz University of Technology, Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Wólczańska 213, 93-005 Łódź, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025)

Typ dokumentu

Bibliografia

Identyfikator YADDA

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