Improved adhesion and growth of osteoblast-like MG-63 cells in cultures on titanium modified by gold particles

• Autorzy: Parizek M. , Base T. , Hruby M. , Lisa V. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

Metallic materials are important for load-bearing bone implants. The osteointegration of these implants can be improved by appropriate surface modifications. Therefore, we present here a study of the cell growth on titanium surfaces modified with films created from gold microparticles. These particles in the form of microplates or polyhedral microcrystals were deposited on titanium plates from ethanol solutions, dried and annealed with a hydrogen flame. Some samples were additionally modified by polyethylene imine. The materials engendered from these modifications were used to investigate the adhesion and growth of human osteoblast-like MG-63 cells on these surfaces in the DMEM medium with 10% of fetal bovine serum. One day after seeding, the highest number of initially adhered cells was found on the surfaces modified by both types of gold microparticles. This trend was the same three and seven days after seeding. The numbers of cells on pure Ti and Ti modified only with gold particles were significantly higher than on samples which were modified with polyethylene imine. The cell spreading areas projected on the materials were significantly larger in cells on the samples with polyethylene imine modification. However, the shape of these cells was mostly rounded or star-like with thin and long protrusions, while on the materials without polyethylene imine, it was mostly polygonal. The cell proliferation activity was estimated from XTT test, based on the activity of mitochondrial enzymes. This test showed that the proliferation activities of osteoblast-like MG-63 cells of the 3rd and 7th days of the experiment were more pronounced on the samples modified only by gold microparticles. Immunofluorescence showed that the focal adhesion plaques containing vinculin and the fibers containing β-actin were most apparent, more numerous and more brightly stained in cells on Ti modified by gold microplates and gold polyhedral microcrystals, especially in comparison with the corresponding samples modified with polyethylene imine (Fig. 1). Thus, it can be concluded that the modification of titanium samples by both types of gold microparticles enhanced the adhesion and growth of MG 63 cells.

Słowa kluczowe

EN

metallic materials; titanium; surgical implants; gold microparticles; cell adhesion; cell growth

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2013
• Tom: Vol. 16, no. 122-123 spec. iss.
• Strony: 77
• Opis fizyczny: zdj.

Twórcy

autor

Parizek M.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Base T.

• Institute of Inorganic Chemistry, Acad. Sci. CR, 250 68 Husinec-Rez 101, Czech Republic

autor

Hruby M.

• Institute of Macromolecular Chemistry, Acad. Sci. CR, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic

autor

Lisa V.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

autor

Bacakova L.

• Institute of Physiology, Acad. Sci. CR, Videnska 1083, 142 20 Prague 4-Krc, Czech Republic

Uwagi

EN

Supported by the Grant Agency ot the Czech Republic (grant No. P108/10/1106) and Technological Agency of the Czech Republic (grant No. TA01011141).