Modelling of a hybrid culture system with a stationary layer of liquid perfluorochemical applied as oxygen carrier

• Autorzy: Pilarek M. , Dąbkowska K.

Identyfikatory

DOI

10.1515/cpe-2016-0014

• Języki publikacji: EN

Abstrakty

EN

A mathematical model of a hybrid culture system supported with a stationary layer of liquid perfluorochemical (PFC) as a source of O2 for cells which grow in the aqueous phase of culture medium has been developed and discussed. The two-substrate Monod kinetics without inhibition effects, i.e. the Tsao-Hanson equation, has been assumed to characterise the biomass growth. The Damköhler number which relates the growth rate to the mass transfer effects has been used to appraise the regime (i.e. diffusion-limited or kinetics) of the whole process. The proposed model predicted accurately previously published data on the submerged batch cultures of Nicotiana tabacum BY-2 heterotrophic cells performed in a culture system supported with a stationary layer of hydrophobic perfluorodecalin as a liquid O2 carrier. Estimated values of the parameters of the model showed that the process proceeded in the kinetics regime and the growth kinetics, not the effects of the mass transfer between aqueous phase and liquid PFC, had essential influence on the growth of biomass.

Słowa kluczowe

EN

liquid perfluorochemical (fluorocarbon); liquid gas carrier; mathematical modelling; cell/tissue culture; hybrid culture system

PL

ciecz perfluorochemiczna fluorowęglowa; ciekły gaz; modelowanie matematyczne; hybrydowy system hodowli

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2016
• Tom: Vol. 37, nr 1
• Strony: 149--158
• Opis fizyczny: Bibliogr. 17 poz., wykr.

Twórcy

autor

Pilarek M.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

autor

Dąbkowska K.

• Warsaw University of Technology, Faculty of Chemical and Process Engineering, Waryńskiego 1, 00-645 Warsaw, Poland

Bibliografia

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