Geographical Origin and Solvent Type Impact on Inula viscosa (L.) Aiton Grown in El Menzel – Morocco – Insights into Bioactivity and Applications

• Autorzy: Tlemcani Sara , El Ouadrhiri Faiçal , Hmamou Anouar , Lahkimi Amal , Touijer Hanane , Kara Mohammed , Idrissi Amine Mounadi , Bekkari Hicham

Identyfikatory

DOI

10.12912/27197050/176119

• Języki publikacji: EN

Abstrakty

EN

Geographical origin and environmental factors have a significant impact on the constituents and the biological properties of medicinal and aromatic plants. Herein, the Inula viscosaplant grown in El Menzel – Morocco were investigated with a focus on the impact of geographical province and solvent type on the mass yield and the biological activities of plant extracts. Chemical composition was characterized by gas chromatography/mass spectrometry (GC/MS). Antimicrobial activity was determined using the disk diffusion method and the microdilution test against eight clinical fungal, Gram-positive and Gram-negative bacterial isolates. The chemical composition results showed that the plant has good nutritional quality in terms of protein, carbohydrates, lipids and dietary fiber. In fact, alkaloids and saponisides are the most predominant chemical compounds in Inula vuscosa. Meanwhile, eighty volatile compounds were identified, representing 95% of the total essential oil content, the main component of which is tetra-pentacontane (11.26%). Furthermore, results showed high antioxidant activity, with efficacy increasing in the order: essential oil > chloroform extract > ethereal extract > ethanolic extract. In addition, both chloroformic extract and essential oil demonstrated significant antibacterial activity against all strains tested. This study highlights the influence of geographical variations and extraction solvents on the bioactivity of Inula viscosa, offering insights into its potential applications in pharmacology and nutraceuticals.

Słowa kluczowe

EN

Inula viscosa; antioxidant activity; antimicrobial activity; photochemical composition

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2024
• Tom: Vol. 25, iss. 2
• Strony: 168--177
• Opis fizyczny: Bibliogr. 36 poz., rys., tab.

Twórcy

autor

Tlemcani Sara

• Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco
• Laboratory of Engineering, Electrochemistry, Modeling and Environment. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0009-0002-9005-8953

autor

El Ouadrhiri Faiçal

• Laboratory of Engineering, Electrochemistry, Modeling and Environment. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0000-0002-1089-0241

autor

Hmamou Anouar

• Laboratory of Engineering, Electrochemistry, Modeling and Environment. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0000-0001-9774-0125

autor

Lahkimi Amal

• Laboratory of Engineering, Electrochemistry, Modeling and Environment. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0000-0001-6830-755X

autor

Touijer Hanane

• Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0000-0001-7511-2363

autor

Kara Mohammed

• Laboratory of Biotechnology, Conservation and Valorization of Natural Resources (LBCVNR), Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco

• https://orcid.org/0000-0002-5102-2379

autor

Idrissi Amine Mounadi

• Laboratory of Engineering, Electrochemistry, Modeling and Environment. Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0009-0001-8036-9558

autor

Bekkari Hicham

• Laboratory of Biotechnology, Environment, Agri-food and Health, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Morocco

• https://orcid.org/0000-0002-8621-1030

Bibliografia

• 1. Abdelkader, O., Messaoud, R., Takia, L., Pierre, C., Gilles, F. 2020. Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria. Acta Scientifica Naturalis, 7(2), 31–43. https://doi.org/10.2478/asn-2020-0018
• 2. Al-Dissi, N.M., Salhab, A.S., Al-Hajj, H.A. 2001. Effects of Inula viscosa leaf extracts on abortion and implantation in rats. Journal of Ethnopharmacology, 77(1), 117–121. https://doi.org/10.1016/S0378-8741(01)00261-6
• 3. Amin, T., Naik, H.R., Hussain, S.Z., Jabeen, A., Thakur, M. 2018. In-vitro antioxidant and antibacterial activities of pumpkin, quince, muskmelon and bottle gourd seeds. Journal of Food Measurement and Characterization, 12(1), 182–190. https://doi.org/10.1007/s11694-017-9629-8
• 4. Balouiri, M., Sadiki, M., Ibnsouda, S.K. 2016. Methods for in Vitro Evaluating Antimicrobial Activity: A Review. J. Pharm. Anal, 6, 71–79.
• 5. Chahmi, N., Anissi, J., Jennan, S., Farah, A., Sendide, K., El Hassouni, M. 2015. Antioxidant activities and total phenol content of Inula viscosa extracts selected from three regions of Morocco. Asian Pacific Journal of Tropical Biomedicine, 5(3), 228–233. https://doi.org/10.1016/S2221-1691(15)30010-1
• 6. Chen, Q., Fung, K.Y., Lau, Y.T., Ng, K.M., Lau, D.T.W. 2016. Relationship between maceration and extraction yield in the production of Chinese herbal medicine. Food and Bioproducts Processing, 98, 236–243. https://doi.org/10.1016/J.FBP.2016.02.005
• 7. Cock, I.E., Selesho, M.I., Van Vuuren, S.F. 2018. A review of the traditional use of southern African medicinal plants for the treatment of selected parasite infections affecting humans. Journal of Ethnopharmacology, 220, 250–264. https://doi.org/10.1016/J.JEP.2018.04.001
• 8. Dzoyem, J.P., Tshikalange, E., Kuete, V. 2013. Medicinal Plants Market and Industry in Africa. Medicinal Plant Research in Africa: Pharmacology and Chemistry, 859–890. https://doi.org/10.1016/B978-0-12-405927-6.00024-2
• 9. El Khomsi, M., Kara, M., Hmamou, A., Assouguem, A., Al Kamaly, O., Saleh, A., Ercisli, S., Fidan, H., Hmouni, D. 2022. In Vitro Studies on the Antimicrobial and Antioxidant Activities of Total Polyphenol Content of Cynara humilis from Moulay Yacoub Area (Morocco). Plants, 11, 1200. https://doi.org/10.3390/plants11091200
• 10. Elyemni, M., El Ouadrhiri, F., Lahkimi, A., Elkamli, T., Bouia, A., Eloutassi, N. 2022. Chemical Composition and Antimicrobial Activity of Essential Oil of Wild and Cultivated Rosmarinus Officinalis from Two Moroccan Localities. Journal of Ecological Engineering, 23(3), 214–222. https://doi.org/10.12911/22998993/145458
• 11. Ozkan, E., Pehlivan Karakas, F., Birinci Yildirim, A., Tas, I., Eker, I., Zeynep Yavuz, M., Ucar Turker, A. 2019. Promising medicinal plant Inula viscosa L. Antiproliferative, antioxidant, antibacterial and phenolic profiles. Progress in Nutrition. DOI: 10.23751/pn.v21i3.7186.
• 12. Farooq, S., Mir, S.A., Shah, M.A., Manickavasagan, A. 2021. Extraction techniques. Plant Extracts: Applications in the Food Industry, 23–37. https://doi.org/10.1016/B978-0-12-822475-5.00005-3
• 13. Gnat, S., Łagowski, D., Nowakiewicz, A., Dyląg, M. 2021. A global view on fungal infections in humans and animals: opportunistic infections and microsporidioses. Journal of Applied Microbiology, 131(5), 2095–2113. https://doi.org/10.1111/jam.15032
• 14. Haoui, I.E., Derriche, R., Madani, L., Oukali, Z. 2015. Analysis of the chemical composition of essential oil from Algerian Inula viscosa (L.) Aiton. Arabian Journal of Chemistry, 8(4), 587–590. https://doi.org/10.1016/J.ARABJC.2011.05.005
• 15. Hmamou, A., Eloutassi, N., Alshawwa, S. Z., Al Kamaly, O., Kara, M., Bendaoud, A., El-Assri, E. M., Tlemcani, S., El Khomsi, M., Lahkimi, A. 2022. Total Phenolic Content and Antioxidant and Antimicrobial Activities of Papaver rhoeas L. Organ Extracts Growing in Taounate Region, Morocco. Molecules, 27(3). https://doi.org/10.3390/molecules27030854
• 16. Hmamou, A., El Khomsi, M., El-Assri, E.-M., Kara, M., El oumari, F.E., El Ouadrhiri, F., Bendaoud, A., Elmansouri, I., Eloutassi, N., Lahkimi, A. 2024. Chemical characterization, anti-struvite crystal, anti-inflammatory, analgesic, and antidepressant activities of Papaver rhoeas L. root and leaf extracts. Journal of Ethnopharmacology, 319, 117208. https://doi.org/10.1016/j.jep.2023.117208
• 17. Hmamou, A., El-Assri, E.-M., El Khomsi, M., Kara, M., Zuhair Alshawwa, S., Al Kamaly, O., El oumari, F.E., Eloutassi, N., Lahkimi, A. 2023a. Papaver rhoeas L. stem and flower extracts: Anti-struvite, anti-inflammatory, analgesic, and antidepressant activities. Saudi Pharmaceutical Journal, 31, 101686. https://doi.org/10.1016/j.jsps.2023.06.019
• 18. Hmamou, A., Kara, M., Khomsi, M.E., Saleh, A., Al Kamaly, O., Bendaoud, A., El Ouadrhiri, F., Adachi, A., Tlemcani, S., Eloutassi, N., Lahkimi, A. 2023b. Comparative Study on the Total Phenolics, Total Flavonoids, and Biological Activities of Papaver rhoeas L. Extracts from Different Geographical Regions of Morocco. Applied Sciences, 13, 2695. https://doi.org/10.3390/app13042695
• 19.Jaiswal, R., Kiprotich, J., Kuhnert, N. 2011. Determination of the hydroxycinnamate profile of 12 members of the Asteraceae family. Phytochemistry, 72(8), 781–790. https://doi.org/10.1016/j.phytochem.2011.02.027
• 20. Kheyar-Kraouche, N., Boucheffa, S., Bellik, Y., Farida, K., Brahmi-Chendouh, N. 2023. Exploring the potential of Inula viscosa extracts for antioxidant, antiproliferative and apoptotic effects on human liver cancer cells and a molecular docking study. Biotechnologia, 104(2), 183–198. https://doi.org/10.5114/bta.2023.127207
• 21. Khomsi, M.E., Hmamou, A., Oubbadi, R.E., El houda Tahiri, N., Kara, M., Amar, A., Mesfioui, A., Hmouni, D. 2024. HPLC analysis and anti-inflammatory, antinociceptive, healing and antidepressant properties of Anchusa italica Retz extracts. Phytomedicine Plus, 4, 100518. https://doi.org/10.1016/j.phyplu.2023.100518
• 22. Khomsi, M.E., Imtara, H., Kara, M., Hmamou, A., Assouguem, A., Bourkhiss, B., Tarayrah, M., Al-Zain, M.N., Alzamel, N.M., Noman, O., Hmouni, D. 2022. Antimicrobial and Antioxidant Properties of Total Polyphenols of Anchusa italica Retz. Molecules, 27, 416. https://doi.org/10.3390/molecules27020416
• 23. Kokina, M., Salevic, A., Kaluševic, A., Levic, S., Pantic, M., Dejan Pljevljakušic, Šavikin, K., Shamtsyan, M., Nikšic, M., Nedovic, V. 2019. Characterization, antioxidant and antibacterial activity of essential oils and their encapsulation into biodegradable material followed by freeze drying. Food Technology and Biotechnology, 57(2), 282–289. https://doi.org/10.17113/ftb.57.02.19.5957
• 24. Labhar, A., Benamari, O., El-Mernissi, Y., Salhi, A., Ahari, M., El Barkany, S., Amhamdi, H. 2023. Phytochemical, Anti-Inflammatory and Antioxidant Activities of Pistacia lentiscus L. Leaves from Ajdir, Al Hoceima Province, Morocco. Ecological Engineering & Environmental Technology, 24(7), 172–177. https://doi.org/10.12912/27197050/169935
• 25. Madani, L., Derriche, R., Haoui, I.E. 2014. Essential oil of Algerian Inula viscosa leaves. Journal of Essential Oil-Bearing Plants, 17(1), 164–168. https://doi.org/10.1080/0972060X.2014.884778
• 26. Mssillou, I., Agour, A., Allali, A., Saghrouchni, H., Bourhia, M., El Moussaoui, A., Salamatullah, A.M., Alzahrani, A., Aboul-Soud, M.A.M., Giesy, J.P. 2022. Antioxidant, Antimicrobial, and Insecticidal Properties of a Chemically Characterized Essential Oil from the Leaves of Dittrichia viscosa L. Molecules, 27, 2282. https://doi.org/10.3390/molecules27072282
• 27. Parolin, P. 2014. Biology of Dittrichia viscosa, a mediterranean ruderal plant: a review. International Journal of Experimental Botany, 83, 251–262. https://hal.inrae.fr/hal-02634395
• 28. Qneibi, M., Hanania, M., Jaradat, N., Emwas, N., Radwan, S. 2021. Inula viscosa (L.) Greuter, phytochemical composition, antioxidant, total phenolic content, total flavonoids content and neuroprotective effects. European Journal of Integrative Medicine, 42, 101291. https://doi.org/10.1016/j.eujim.2021.101291
• 29.Rai, V. 2002. Role of Amino Acids in Plant Responses to Stresses. Biologia Plantarum, 45, 481– 487. https://doi.org/10.1023/A:1022308229759
• 30. Silva, D.B., Okano, L.T., Lopes, N.P., De Oliveira, D.C.R. 2013. Flavanone glycosides from Bidens gardneri Bak. (Asteraceae). Phytochemistry, 96, 418–422. https://doi.org/10.1016/j.phytochem.2013.09.024
• 31. Srivastava, N., Singh, A., Kumari, P., Nishad, J.H., Gautam, V.S., Yadav, M., Bharti, R., Kumar, D., Kharwar, R.N. 2021. Advances in extraction technologies: isolation and purification of bioactive compounds from biological materials. Natural Bioactive Compounds: Technological Advancements, 409–433. https://doi.org/10.1016/B978-0-12-820655-3.00021-5
• 32. Subramanian, P., Anandharamakrishnan, C. 2023. Extraction of bioactive compounds. Industrial Application of Functional Foods, Ingredients and Nutraceuticals, 45–87. https://doi.org/10.1016/B978-0-12-824312-1.00002-9
• 33. Tlemcani, S., Lahkimi, A., Eloutassi, N., Bendaoud, A., Hmamou, A., Bekkari, H. 2023. Ethnobotanical study of medicinal plants in the Fez-Meknes region of Morocco. J.. Pharm Res, 11, 137-159.
• 34. Werker, E., Fahn, A. 1981. Secretory hairs of Inula viscosa (L.) Ait. - development, ultrastructure, and secretion. Botanical Gazette, 142(4), 461–476. https://doi.org/10.1086/337247
• 35. Yildirim, K., Atas, C., Simsek, E., Coban, A.Y. 2023. The Effect of Inoculum Size on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis. Microbiology Spectrum, 11(3). https://doi.org/10.1128/spectrum.00319-23
• 36. Zeouk, I., Sifaoui, I., Ben Jalloul, A., Bekhti, K., Bazzocchi, I.L., Piñero, J.E., Jiménez, I.A., Lorenzo-Morales, J. 2022. Isolation, identification, and activity evaluation of antioxidant components from Inula viscosa: A bioguided approach. Bioorganic Chemistry, 119, 105551. https://doi.org/10.1016/J.BIOORG.2021.105551

Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).