Aerobic biodegradation of norfloxacin and ofloxacin by a microbial consortium

• Autorzy: Jałowiecki Łukasz , Płaza Grażyna , Ejhed Helena , Nawrotek Monika

Identyfikatory

DOI

10.24425/aep.2019.130240

Warianty tytułu

PL

Kompost jako źródło mikroorganizmów biorących udział w biodegradacji norfloksacyny i ofloksacyny

• Języki publikacji: EN

Abstrakty

EN

Since fluoroquinolone (FQ) antibiotics are extensively used both in human and veterinary medicine their accumulation in the environment is causing increasing concern. The aim of the study was to isolate a microbial consortium resistant to ofloxacin and norfloxacin and able to biodegrade both antibiotics. Green compost was used as a source of microorganisms. The biodegradation efficiency was monitored by changes of antibiotics concentrations and toxicity. The microbial consortium was composed of two bacterial isolates: Klebsiella pneumoniae (K2) and Achromobacter sp. (K3) and two fungi Candida manassasensis (K1) and Trichosporon asahii (K4). All the isolates were characterized as highly resistant to both antibiotics – ofloxacin and norfloxacin. FQs were supplied individually into the culture medium in the presence of an easily degradable carbon source – glucose. Biodegradation of norfloxacin was much faster than ofloxacin biodegradation. During 20 days of the experiment, the norfloxacin level decreased by more than 80%. Ofloxacin was generally biodegraded thereafter at relatively slow biodegradation rate. After 28 days the ofloxacin level decreased by 60%. Similarly, the toxicity of biodegraded antibiotics decreased 4-fold and 3.5-fold for norfloxacin and ofloxacin, respectively. The ability of the bacterial-fungal consortium to degrade antibiotics and reduce toxicity could help to reduce environmental pollution with these pharmaceutical.

PL

Antybiotyki to zróżnicowana grupa związków, która nie ma konkretnych uregulowań prawnych, dotyczących ich występowania w środowisku, zarówno wodnym jak i glebowym. Farmaceutyki przedostają się do środowiska m.in. wraz ze ściekami oczyszczonymi z oczyszczalni ścieków i jako substancje czynne biologicznie stanowią poważne zagrożenie dla organizmów żywych. Ich akumulacja w środowisku prowadzi do nieodwracalnych zmian w ekosystemach oraz szerzenia się zjawiska oporności wśród mikroorganizmów. Fluorochinolony (FQ) to syntetyczne substancje antybakteryjne o zwiększonym potencjale farmakokinetycznym i szerokim spektrum działania. FQ to jedna z najszybciej rozwijających się klas antybiotyków coraz częściej stosowanych zarówno w szpitalach, jak i w społecznościach lokalnych w leczeniu różnego typu zakażeń. Norfloksacyna i ofloksacyna to FQ II generacji o podobnej budowie strukturalnej wykazujące aktywność głównie wobec bakterii Gram-ujemnych. Ze względu na swoja budowę antybiotyki te w niewielkim stopniu są rozkładane w środowisku, przez długi czas kumulują się w wodzie i w glebie, oddziałując w na organizmy żywe. Celem pracy była ocena toksyczności ofloksacyny i norfloksacyny po biodegradacji przez zespół mikroorganizmów wyizolowany z kompostu. Proces biodegradacji przeprowadzono w bioreaktorach New Brunswick™ BioFlo® 415 o pojemności 5,5 l. Stopień degradacji określono za pomocą chromatografi i cieczowej w odwróconym układzie faz. Do oceny toksyczności wykorzystano test Microtox® oparty na pomiarach aktywności bakterii luminescencyjnych Vibrio fischeri.

Słowa kluczowe

EN

biodegradation; antibiotics; norfloxacin; ofloxacin; bacterial-fungal consortium; green compost

PL

biodegradacja; antybiotyki; norfloksacyna; ofloksacyna; konsorcjum bakteryjno-grzybicze; zielony kompost

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2019
• Tom: Vol. 45, no. 4
• Strony: 40--47
• Opis fizyczny: Bibliogr. 30 poz., rys., tab., wykr.

Twórcy

autor

Jałowiecki Łukasz

• Institute for Ecology of Industrial Areas, Poland

autor

Płaza Grażyna

• Faculty of Organization and Management, Silesian University of Technology, Poland

autor

Ejhed Helena

• Swedish Environmental Research Institute, Sweden

autor

Nawrotek Monika

• Institute for Ecology of Industrial Areas, Poland

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