Development and validation of RP HPLC method for the estimation of methyl paraben sodium and propyl paraben sodium in iron protein succinylate syrup

• Autorzy: Javed Muhammad Faisal , Zahra Manzar , Javed Iqra , Ahmad Shahbaz , Jabeen Tayyaba , Ahmad Muhammad

Identyfikatory

DOI

10.1556/1326.2022.00990

• Języki publikacji: EN

Abstrakty

EN

A new precise, selective and reliable reversed phase high performance liquid chromatographic (RP-HPLC) method has been developed and validated for the determination of Methyl paraben sodium (MPS) and Propyl paraben sodium (PPS) (preservatives) in Iron protein succinylate syrup. Optimized conditions were; Methanol: Water (65: 35) as mobile phase, UV/Vis detector at the wavelength of 254 nm and flow rate was set at 1.3 ml min⁻¹. By applying the set of conditions, separation of components was carried out in less than 7 min for both the analytes. The method was validated according to International conference of Harmonization (ICH) guidelines and the analytical characteristic parameters of validation included specificity, limit of detection (LOD), limit of quantification, linearity, accuracy, precision and robustness were evaluated. The calibration curve was found to be linear in the range of 0.045 mg mL⁻¹ to 0.075 mg mL⁻¹ for Methyl paraben sodium and 0.015 mg mL⁻¹ to 0.025 mg mL⁻¹ for propyl paraben sodium with a correlation coefficient r² > 0.999. Accuracy; reported as percentage recovery was found to be in the range of 98.71%–101.64% for Methyl paraben sodium and 99.85%–101.47% for Propyl paraben sodium at 80%, 100% and 120% concentration for both the analytes. The proposed method was found to be precise and robust when evaluated by variations in wavelength, mobile phase composition, temperature and analyst. The limit of detection (LOD) was found 0.001 mg mL⁻¹ (3 ppm) for Methyl paraben sodium and 0.001 mg mL⁻¹ (1 ppm) for Propyl paraben sodium.

Słowa kluczowe

EN

preservatives; limit of detection; limit of quantification; robustness; accuracy

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 1
• Strony: 52--59
• Opis fizyczny: Bibliogr. 37 poz., tab., rys., wykr.

Twórcy

autor

Javed Muhammad Faisal

• Department of Chemistry, Lahore Garrison University, Lahore, Pakistan

autor

Zahra Manzar

• Department of Chemistry, Lahore Garrison University, Lahore, Pakistan

• https://orcid.org/0000-0002-7033-0254

autor

Javed Iqra

• Lahore Pharmacy College, A Project of Lahore Medical & Dental College, Pakistan

autor

Ahmad Shahbaz

• Himont Parmaceuticals, Lahore, Pakistan

autor

Jabeen Tayyaba

• Department of Zoology, Quaid-i-Azam University, Islamabad, Pakistan

autor

Ahmad Muhammad

• Department of Chemistry, Division of Science and Technology, University of Education, Lahore, 54770, Pakistan

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).