Determination of ampicillin and amoxicillin by high-performance thin-layer chromatography

• Autorzy: Ghoulipour V. , Shokri M , Waqif-Husain S.
• Języki publikacji: EN

Abstrakty

EN

A simple and fast high-performance thin-layer chromatographic method has been developed for the simultaneous determination of ampicillin and amoxicillin. Titanium(IV) silicate (a synthetic inorganic ion-exchanger)-coated thin-layer chromatography (TLC) plates were used to separate them, employing a mixture of K2HPO4 (0.1 M) + KH2PO4 (0.1 M), 1:1 (υ/υ), as mobile phase. The development time was 18 min. The plates were sprayed with fresh 1% solution of ninhydrin in ethanol. The developed method enables highly contrasted chromatograms with red purple spots in white background. Densitometric measurements were made at wavelength 546 nm using Camag TLC Scanner-3. The ampicillin and amoxicillin recovery of the total procedure were equal to 99.99 and 100.43, respectively. The procedure is quantitatively characterized. Linearities were r2 > 0.9958 and 0.9954 for ampicillin and amoxicillin, respectively, and the relative standard deviations were <0.89 and 0.61, respectively. The limits of detection were 2.9 and 1.5 ng per spot and the limits of quantification were 14.5 and 7.5 ng per spot, respectively. The method is rapid, selective, precise, and accurate and thus can be used for the routine analysis of pharmaceutical preparations in quality control laboratories of the pharmaceutical industry. The method is successfully applied for the determination of ampicillin and amoxicillin in human blood plasma and urine.

Słowa kluczowe

EN

ampicillin; amoxicillin; titanium(IV) silicate; HPTLC; densitometry

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 3
• Strony: 483--498
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Ghoulipour V.

• University of Tarbiat Moallem Department of Applied Chemistry, Faculty of Chemistry 49 Mofatteh Avenue Tehran 15614 Iran

autor

Shokri M

• University of Tarbiat Moallem Department of Applied Chemistry, Faculty of Chemistry 49 Mofatteh Avenue Tehran 15614 Iran

autor

Waqif-Husain S.

• Islamic Azad University Department of Chemistry, Faculty of Science, Science and Research Branch P.O. Box 14515-775 Poonak-Hesarak, Tehran Iran

Bibliografia

• [1] G.L. Mandell, R.G. Douglas Jr., and J.E. Bennett (Eds) Principles and Practice of Infectious Diseases, 3rd edn, Churchill Livingstone, New York, 1990, pp. 240–242
• [2] M. Becker, E. Zittlau, and M. Petz, Anal. Chim. Acta, 520, 19 (2004)
• [3] K. Bush. In: F. O’Grady, H.P. Lambert, R.G. Finch, and D. Greenwood (Eds), Antibiotic and Chemotherapy: Anti-Infective Agents and Their Use in Therapy, Churchill Livingstone, New York, 1997, pp. 306–309
• [4] V. Gamba and G. Dusi, Anal. Chim. Acta, 483, 69 (2003)
• [5] L. Basaez and P. Vanysek, J. Pharm. Biomed. Anal., 19, 183 (1999)
• [6] L. Xu, H. Wang, and Y. Xiao, Spectrochim. Acta A, 60, 3007 (2004)
• [7] G.G. Mohamed, J. Pharm. Biomed. Anal., 24, 561 (2001)
• [8] A. Garcia-Reiriz, P.C. Damiani, and A.C. Olivieri, Talanta, 71, 806 (2007)
• [9] H. Salem, Anal. Chim. Acta, 515, 333 (2004)
• [10] H.J. Mascher and C. Kikutu, J. Chromatogr., A, 812, 221 (1998)
• [11] L.R.P. Abreu, R.A.M. Ortiz, S.C. Castro, and J. Pedrazzoli, J. Pharm. Pharmaceut. Sci., 6(2), 223 (2003)
• [12] B. Deng, A. Shi, L. Li, and Y. Kang, J. Pharm. Biomed. Anal., 48, 1249 (2008)
• [13] A. Ramirez, R. Gutierrez, G. Diaz, C. Gonzalez, N. Perez, S. Vega, and M. Noa, J. Chromatogr. B, 784, 315 (2003)
• [14] G. Pajchel, K. Pawlowski, and S. Tyski, J. Pharm. Biomed. Anal., 29, 75 (2002)
• [15] E.S. Elmolla and M. Chaudhuri, J. Hazard. Mater., 173, 445 (2010)
• [16] S.W. Husain, V. Ghoulipour, and H. Sepahrian, Acta Chromatogr., 14, 102 (2004)
• [17] Z. Hassankhani-Majd, V. Ghoulipour, and S.W. Husain, Acta Chromatogr., 16, 173 (2006)
• [18] K. Purghazi, V. Ghoulipour, S.W. Husain, and A. Mirzaie, Acta Chromatogr., 18, 219 (2007)
• [19] J. Sherma, Anal. Chem., 72(12) 9R–25R (2000); 74 (12) 2653–2662 (2002); 76 (12) 3251–3261 (2004); 78 (12) 3841–3852 (2006); 80 (12) 4253–4267 (2008)
• [20] F. Kreuzig. In: J. Sherma and B. Fried (Eds), Handbook of Thin Layer Chromatography, Dekker, New York, 1990, pp. 407–417
• [21] S.P. Kumar, P.V. Srinivas, and J.M. Rao, J. Planar Chromatogr., 15, 128 (2002)
• [22] V. Ghoulipour and S.W. Husain, J. Planar Chromatogr., 19, 246 (2006)
• [23] V. Ghoulipour and S.W. Husain, Acta Chromatogr., 12, 170 (2002)
• [24] V. Ghoulipour and S.W. Husain, Anal. Sci., 16, 1079 (2000)
• [25] V. Ghoulipour and S.W. Husain, J. Planar Chromatogr., 13, 354 (2000)
• [26] ICH Guidelines Q2B, Validation of Analytical Procedures: Methodology (CPMP/ICH/281/95)
• [27] E. Papp, A. Farkas, K.H. Otta, and E. Mincsovics, J. Planar Chromatogr., 13, 328 (2000).
• [28] K. Ferenczi-Fodor, A. Nagy-Turak, and Z. Vegh, J. Planar Chromatogr., 8, 349 (1995)
• [29] B. Renger, H. Jehle, M. Fischer, and W. Funck, J. Planar Chromatogr., 8, 269 (1995)
• [30] C.F. Poole, S.K. Poole, and T.A. Dean, Recent Advances in Thin Layer Chromatography. In: F.A.A. Dallas, H. Read, R.J. Ruane, and I. D. Wilson (Eds), Plenum, New York, 1988, p. 11
• [31] A. Ramirez, R. Gutierrez, G. Diaz, C. Gonzalez, N. Perez, S. Vega, and M. Noa, J. Chromatogr. B, 784, 315 (2003)