Separation of betalains by gradient high-speed counter-current chromatography

• Autorzy: Spórna-Kucab A. , Udziela D. , Sobocińska M. , Szot D. , Wybraniec S.
• Języki publikacji: EN

Abstrakty

EN

A study on separation of betalain mixture obtained from red beet juice (Beta vulgaris L.) by analytical high-speed counter-current chromatography (HSCCC) was performed. The extract was obtained by thermal treatment of acidified red beet juice for 30 min in 85 °C. The pigment mixture consisted of betanin/isobetanin as well as their decarboxy- and dehydro-derivatives. The HSCCC process was accomplished in the ‘tail to head’ mode with two polar solvent systems containing salt: BuOH-EtOH-NaClsolution-H2OH3PO4 (1300:700-1000:1300:700:2.5-5.5 (system I), 1300:200-400:1300:700:2.5-4.5 (system II); v/v/v/v/v). The retention of the stationary phase was 73% (system I) and 79% (system II). The mobile phase was pumped at 2 ml/min flow rate. HPLC-DAD-ESI-MS analyses were performed in reversed phase mode for the obtained HSCCC fractions and crude extract. The solvent systems enabled separation of betanin and decarboxy-betanins (system I and II) as well as neobetanin (system II). Additionally, some pure fractions of 17-decarboxy-betanin and 2,17-bidecarboxy-betanin were obtained in system II.

Słowa kluczowe

EN

betanin; betalains; betacyanins; high-speed counter-current chromatography; Beta vulgaris L.

PL

betanina; betalainy; betacyjaniny; HSCCC; Beta vulgaris L.

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2016
• Tom: Vol. 7, no. 1
• Strony: 23--28
• Opis fizyczny: Bibliogr. 10 poz., rys., wykr., tab.

Twórcy

autor

Spórna-Kucab A.

• Cracow University of Technology, Department of Analytical Chemistry, Institute C-1, Warszawska 24, 31-155 Cracow, Poland

autor

Udziela D.

• Cracow University of Technology, Department of Analytical Chemistry, Institute C-1, Warszawska 24, 31-155 Cracow, Poland

autor

Sobocińska M.

• Cracow University of Technology, Department of Analytical Chemistry, Institute C-1, Warszawska 24, 31-155 Cracow, Poland

autor

Szot D.

• Cracow University of Technology, Department of Analytical Chemistry, Institute C-1, Warszawska 24, 31-155 Cracow, Poland

autor

Wybraniec S.

• Cracow University of Technology, Department of Analytical Chemistry, Institute C-1, Warszawska 24, 31-155 Cracow, Poland

Bibliografia

• [1] Strack, D., Vogt, T., Schliemann, W. “Recent advances in betalain research”. Phytochemistry, 62, (2003): 247-269.
• [2] Wybraniec, S., Stalica, P., Spórna, A., Nemzer, B., Pietrzkowski, Z., Michałowski, T. “Antioxidant activity of betanidin: electrochemical study in aqueous media”. Journal of Agricultural and Food Chemistry, 59, (2011): 12163-12170.
• [3] Spórna-Kucab, A., Garrard, I., Ignatova, S., Wybraniec, S. „New solvent systems for gradient counter-current chromatography in separation of betanin and its derivatives from processed Beta vulgaris L”. juice. Journal of Chromatography A, 13 80, (2015):29-37.
• [4] Wybraniec, S. “A method for identification of diastereomers of 2-decarboxy-betacyanins and 2,17-bidecarboxy-betacyanins in reversed phase HPLC”. Analytical and Bioanalytical Chemistry, 389, (2007): 1611-1621.
• [5] Wybraniec, S. Formation of decarboxylated detacyanins in heated purified betacyanin fractions from red beet root (Beta vulgaris L.). Journal of Agricultural and. Food Chemistry, 53, (2005): 3483–3487.
• [6] Degenhardt, A., Winterhalter, P. “Isolation of natural pigments by high speed CCC”. Journal of Liquid Chromatography and Related Technologies, 24, (2001): 1745-1764.
• [7] Spórna-Kucab, A., Ignatova, S., Garrard, I., Wybraniec, S. “Versatile solvent systems for the separation of betalains from processed Beta vulgaris L. juice using counter-current chromatography”. Journal of chromatography B, 941 (2013): 54-61.
• [8] Wybraniec, S., Stalica, P., Jerz, G., Klose, B., Gebers, N., Winterhalter, P., Spórna, A., Szaleniec, M., Mizrahi Y. “Separation of polar betalain pigments from cacti fruits of Hylocereus polyrhizus by ion-pair high-speed countercurrent chromatography. Journal of Chromatography A, 1216, (2009): 6890-6899.
• [9] Ito, Y., Conway, W., D. “High-speed countercurrent chromatography”. Chemical Analysis, tom 132, Wiley-Interscience, New York (1993): 3-44.
• [10] Berthod, A. “Countercurrent chromatography”. Elsevier, Amsterdam, (2002): 1-37.

Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.