First detailed quantification of silymarin components in the leaves of Silybum marianum cultivated in egypt during different growth stages

• Autorzy: Omar A. A , Hadad G. M , Badr J. M
• Języki publikacji: EN

Abstrakty

EN

A high performance liquid chromatographic method was developed for the quantitative determination of the active components of silymarin in the leaves of Silybum marianum during different growth stages. In this study, taxifolin and six main active constituents in silymarin, including silydianin, silychristin, diastereomers of silybin (silybin A and B) and diastereomers of isosilybin (isosilybin A and B) were completely separated on a 5-μm ODS column (Luna, Phenomenex, USA) with a mobile phase consisting of methanol and 5 mM NaH2PO4 (pH 3.5 adjusted with phosphoric acid) in a ratio of 45:55 v/v. Quantitation was performed with UV detection at 280 nm, based on peak area. The concentration of each component, as well as the total silymarin concentration was determined and compared with those of the seeds, aiming at optimizing the utilization of the cultivated plant. The developed method was validated with respect to linearity, range, specificity, accuracy, precision, and robustness. The leaves were found to contain such concentrations of silymarin components that the yield is better per field area than that from the seeds. Moreover, the extraction of these components from leaves is nonexpensive and simpler than the extraction from seeds.

Słowa kluczowe

EN

Silybum marianum; flavolignans; leaves; growth stages; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 3
• Strony: 463--473
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Omar A. A

• Alexandria University Department of Pharmacognosy, Faculty of Pharmacy Alexandria 21521 Egypt

autor

Hadad G. M

• Suez Canal University Department of Analytical Chemistry, Faculty of Pharmacy Ismailia 41522 Egypt

autor

Badr J. M

• King Abdulaziz University Department of Natural Products and Alternative Medicine, Faculty of Pharmacy Jeddah 21589 Kingdom of Saudi Arabia
• 4Suez Canal University Department of Pharmacognosy, Faculty of Pharmacy Ismailia 41522 Egypt

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