Study of retention of 31 polyoxygenated steroids by normal- and reversed-phase thin-layer chromatography

• Autorzy: Tosti T , Natić M , Smoliński A. , Milić D , Milojković-Opsenica D , Tešić Ž
• Języki publikacji: EN

Abstrakty

EN

The chromatographic behavior of 31 steroidal compounds on both silica gel and RP-18 silica thin layers using nonaqueous and aqueous-organic mobile phases was investigated. It was found that an application of more polar solvent systems such as acetonitrile-dichloromethane in comparison to acetone-n-hexane resulted in stronger elution of the investigated substances in all investigated cases. Under normal-phase conditions better selectivity was achieved in acetonitrile-dichloromethane and the best in binary mixture with component ratio 1:9 (υ/υ). The selectivity of the separation under reversed-phase conditions was increased with the increased fraction of water in mobile phases used. A conclusion on the influence of substance moiety on the retention behavior of the investigated substances under reversed-phase conditions could not be drawn due to complex retention mechanism. In order to find similarities and dissimilarities between normal- and reversed-phase systems used, retention parameters were investigated by principal component analysis and cluster analysis.

Słowa kluczowe

EN

estrone-derived synthetic steroids; principal component analysis; hierarchical cluster analysis

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 3
• Strony: 429--445
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Tosti T

• University of Belgrade Faculty of Chemistry P.O. Box 51 11158 Belgrade Serbia

autor

Natić M

• University of Belgrade Faculty of Chemistry P.O. Box 51 11158 Belgrade Serbia

autor

Smoliński A.

• Central Mining Institute Department of Energy Saving and Air Protection Plac Gwarków 1 40-166
• Central Mining Institute Department of Energy Saving and Air Protection Plac Gwarków 1 40-166 Katowice Poland

autor

Milić D

• University of Belgrade Faculty of Chemistry P.O. Box 51 11158 Belgrade Serbia

autor

Milojković-Opsenica D

• University of Belgrade Faculty of Chemistry P.O. Box 51 11158 Belgrade Serbia

autor

Tešić Ž

• University of Belgrade Faculty of Chemistry P.O. Box 51 11158 Belgrade Serbia

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