Deep biosphere in the Main Dolomite (Upper Permian) hydrocarbon reservoir

• Autorzy: Borkowski Andrzej , Zdanowski Paweł , Działak Paweł , Kowalski Tomasz , Segit Tomasz

Identyfikatory

DOI

10.24425/agp.2024.152653

• Języki publikacji: EN

Abstrakty

EN

This study focuses on evaluating microbial activity in Main Dolomite reservoir rocks (Zechstein, Wuchiapingian– Permian) at 100°C. Core samples from two wells, one potentially microbiologically active (C-1 well, 100°C) and the other considered inactive due to high temperature (L-1 well, 155.5°C), were analysed. The core from L-1, treated similarly to C-1, was used to control for contamination. Microbial experiments and molecular analyses were performed on both core samples to ensure accurate results unaffected by laboratory processing contamination. Microbial incubation tests were successfully employed to demonstrate microbial hydrocarbon degradation and methane formation from 13C-labelled acetate. A new method for staining microorganisms from rock samples was introduced to estimate biomass. The results indicated that microorganisms from the C-1 well exhibited both hydrocarbon biodegradation and acetoclastic methanogenesis during a 3-month incubation at 100°C. Fluorescence-stained and countable microorganisms were only observed in the C-1 samples, while no biodegradation or methanogenesis occurred in reference samples from L-1 well. The study provides valuable insights into microbial activities in extreme conditions, emphasizing the importance of proper controls and techniques to ensure accurate interpretation of results.

Słowa kluczowe

EN

deep biosphere; Main Dolomite; Microbial activity; Reservoir rock; Zechstein; Permian

PL

głęboka biosfera; dolomit główny; aktywność mikrobiologiczna; skała zbiornikowa; Cechsztyn; Perm

• Wydawca: Faculty of Geology of the University of Warsaw ; Komitet Nauk Geologicznych PAN
• Czasopismo: Acta Geologica Polonica
• Rocznik: 2024
• Tom: Vol. 74, no. 4
• Strony: art. no. e32
• Opis fizyczny: Bibliogr. 63 poz., rys., wykr.

Twórcy

autor

Borkowski Andrzej

• Faculty of Geology, Geophysics and Environmental Protection, AGH University of Krakow

autor

Zdanowski Paweł

• Exploration and Production Branch in Warsaw, Polish Oil and Gas Company, Orlen SA

autor

Działak Paweł

• Faculty of Geology, Geophysics and Environmental Protection, AGH University of Krakow

autor

Kowalski Tomasz

• Faculty of Geology, Geophysics and Environmental Protection, AGH University of Krakow

autor

Segit Tomasz

• University of Warsaw, Faculty of Geology

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2026).