TLC–Densitometry analysis of indole compounds in mycelial culture of Imleria badia and Agaricus bisporus enriched with precursors — serine or anthranilic acid

• Autorzy: Opoka W. , Kała K. , Krężałek R. , Sułkowska-Ziaja K. , Maślanka A. , Muszyńska B.

Identyfikatory

DOI

10.1556/1326.2017.00325

• Języki publikacji: EN

Abstrakty

EN

Agaricus bisporus and Imleria in vitro cultures were cultivated on modified Oddoux medium, and Oddoux medium was enriched with serine or anthranilic acid. Serine or anthranilic acid was used at the concentrations of 0.1, 0.25, 0.5, and 0.75 g/L of medium. Determination of indole compounds in the obtained biomass was carried out using thin-layer chromatography (TLC) with densitometric detection. In every analyzed sample, presence of serine or anthranilic acid was studied. Comparison of the results obtained for the treatment and control samples allowed us to determine the optimum concentration of serine or anthranilic acid in the medium in order to obtain biomass with increased content of indole compounds. A. bisporus with addition of anthranilic acid or serine to the medium at the concentration of 0.5 g/L was the most beneficial. In the case of Imleria badia, anthranilic acid at the concentration of 0.5 g/L was the most optimal. This is the first report demonstrating the content of indole derivatives in biomass affected by their precursors (serine or anthranilic acid). The study indicates that modification of the medium can provide satisfactory results, and it is worth to search for its new, improved compositions.

Słowa kluczowe

EN

Agaricus bisporus; Imleria badia; indole compounds; serine; anthranilic acid; mycelial in vitro cultures

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 4
• Strony: 236--242
• Opis fizyczny: Bibliogr. 28 poz., rys.

Twórcy

autor

Opoka W.

• Department of Inorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

autor

Kała K.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

autor

Krężałek R.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

autor

Sułkowska-Ziaja K.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

autor

Maślanka A.

• Department of Inorganic Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

autor

Muszyńska B.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

Bibliografia

• [1] Muszyńska B., Jadalne gatunki grzybów źródłem substancji dietetycznych i leczniczych; Ośrodek Umea Shinoda – Kuracejo: Krakow, 2012, p. 109.
• [2] Kała K.; Muszyńska B.; Zając M.; Krężałek R.; OpokaW. Int. J. Med. Mushrooms 2016, 18, 155.
• [3] Muszyńska B.; Komendacki P.; Kała K.; OpokaW.; RojowskiJ. Med. Inter. Rev. 2014, 2, 82.
• [4] Muszyńska B. and Sułkowska-Ziaja K. Food Chem. 2012, 132, 455.
• [5] Muszyńska B.; Sułkowska-Ziaja K.; Ekiert H. J. Food Sci. Tech. 2013, 50, 1233.
• [6] Muszyńska B.; Sułkowska-Ziaja K.; Wójcik A. Mycoscience 2013, 54, 321.
• [7] Muszyńska B.; Sułkowska-Ziaja K.; Maślanka A.; Rojowski J.; Opoka W.; Łojewski M. Acta Chromatogr. 2016, 28, 223.
• [8] Heleno S. A.; Ferreira R. C.; AntonioA. L.; Queiroz M. J. R. P.; Barros L.; Ferreira I. C. F. R. Food Biosci. 2015, 11, 48.
• [9] Jaworska G.; Pogoń K.; Skrzypczak A.; Bernaś E. J. Food Sci. Tech. 2015, 52, 7944.
• [10] Kojta A. K. and Falandysz J. Food Chem. 2016, 200, 206.
• [11] Muszyńska B.; Sułkowska-ZiajaK.; Ekiert H. Acta Sci. Pol-Hortoru. 2013, 12, 107.
• [12] Falandysz J.; Zalewska T.; Krasińska G.; Apanel A.; Wang Y.; Pankavec S. Appl. Microbiol. Biot. 2015, 99, 8217.
• [13] Muszyńska B.; Sułkowska-ZiajaK.; Łojewski M.; Opoka W.; Zając M.; RojowskiJ. Med. Inter. Rev. 2013, 101, 170.
• [14] Elmastas M.; Isildak O.; Turkekul I.; Temur N. J. Food Compos. Anal. 2007, 20, 337.
• [15] CheahI. K. and Halliwell B. BBA-Mol. Cell Basis Dis. 2012, 1822, 784.
• [16] ChenS. Y.; Ho K. J.; Hsieh Y. J.; Wang L. T.; Mau J. L. LWT Food Sci. Technol. 2012, 47, 274.
• [17] Calvo M. S.; Mehrotra A.; Beelman R. B.; Nadkarni G.; Wang L.; Cai W.; Goh B. Ch.; Kalaras M. D.; Uribarri J. Plant Food Hum. Nutr. 2016, 71, 245.
• [18] Batterbury M.; Tebbs C. A.; Rhodes J. M.; Grierson I. Exp. Eye Res. 2002, 74, 361.
• [19] Chang H. H.; Chien P. J.; Tong M. H.; Sheu F. Food Chem. 2007, 105, 597.
• [20] Greene W. C.; Fleisher T. A.; Waldmann T. A. J. Immunol. 1981, 126, 580.
• [21] Parslew R.; Jones K. T.; Rhodes J. M.; Sharpe G. R. Brit. J. Dermatol. 1999, 140, 56.
• [22] Wang H. X. and NgT. B. Planta Med. 2001, 67, 669.
• [23] Yu L.; Fernig D. G.; Smith J. A.; Milton J. D.; Rhodes J. M. Cancer Res. 1993, 53, 4627.
• [24] Muszyńska B.; Sułkowska-Ziaja K.; Hałaszczuk P.; Krężałek R.; Łojewski M. Folia Biol. Oecol. 2014, 10, 66.
• [25] Oddoux L., Recherches sur les mycéliums secondaires des Homobasidiés en culture pure; Imprimerie de Trevoux: Lyon, 1957.
• [26] Muszyńska B.; Sułkowska-ZiajaK.; EkiertH. Pharmazie 2009, 64, 479.
• [27] ICH-Q2 (R1) Validation and Analytical Procedures: Text and Methodology, International Conference on Harmonization, Geneva, November 2005.
• [28] Komsta L. J. AOAC Int. 2012, 95, 669.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).