Green versatile micellar electrokinetic chromatographic method for determination of six antimicrobial and anti-inflammatory drugs in combined dosage forms

• Autorzy: Elmansi Heba , El-Awady Mohamed I. , Barghash Sona , El-Razeq Sawsan Abd , Belal Fathalla

Identyfikatory

DOI

10.1556/1326.2022.01057

• Języki publikacji: EN

Abstrakty

EN

A fast reliable micellar electrokinetic methodology was investigated for the concurrent quantitation of six antimicrobial and anti-inflammatory drugs, namely, ciprofloxacin, dexamethasone, metronidazole, ornidazole, spiramycin and tinidazole. The method has the merits of rapidity, precision, and sensitivity. The separation was carried out in less than 7 min by applying a basic background electrolyte consisting of 25 mM disodium tetraborate buffer, pH 9 containing 50 mM SDS at 25 kV using photodiode array detector at 230 and 315 nm. The internal standard used during analysis was cromolyn sodium and validation was carried out following ICH guidelines. The proposed method showed linear response over the range from 0.5 to 10.0 μg mL⁻¹ reaching limits of detection and limits of quantitation in the ranges of 0.09–0.2 μg mL⁻¹ and 0.27–0.6 respectively. The method's greenness was estimated using the GAPI tool where excellent greenness was concluded. Co-formulated or single-ingredient commercial preparations were investigated and the results were statistically evaluated.

Słowa kluczowe

EN

micellar electrokinetic chromatography; ciprofloxacin; dexamethasone; metronidazole; ornidazole

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 3
• Strony: 233--246
• Opis fizyczny: Bibliogr. 68 poz., rys., tab., wykr.

Twórcy

autor

Elmansi Heba

• Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

• https://orcid.org/0000-0002-3953-7169

autor

El-Awady Mohamed I.

• Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt
• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, International Coastal Road, Gamasa, 11152, Egypt

• https://orcid.org/0000-0003-2675-7530

autor

Barghash Sona

• Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

autor

El-Razeq Sawsan Abd

• Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo, Egypt

autor

Belal Fathalla

• Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt

• https://orcid.org/0000-0001-6242-2178

Bibliografia

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