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Despite the disadvantages of glutaraldehyde (GA)-stabilization of tissues, it is the method most often used for xenogeneic tissues preparation. Nowadays, partial elimination of drawbacks of this method is achieved by using GA in the mixture with other crosslinking reagents, which completes the stabilization effects and acts synergistically. The aim of this work was to determine microstructure and nanostructure of porcine pericardium stabilized by GA and tannic acid (TA). The microstructure was examined by optical microscopy and the nanostructure by atomic force microscopy (AFM). Different results on the level of micro- and nanostructure were observed. No essential changes in the tissue morphology after crosslinking with GA and TA were observed under optical microscope, but significant morphological differences were revealed in AFM studies.
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32--34
Opis fizyczny
Bibliogr. 9 poz., wykr., zdj.
Twórcy
autor
- Department of Biopharmacy, Medical University of Silesia, Sosnowiec, Poland
autor
- Centre of Polymer and Carbon Materials, Polish Academy of Science, Zabrze, Poland
autor
- Department of Environmental Biotechnology, Silesian University of Technology, Gliwice, Poland
autor
- Foundation for Cardiac Surgery Development, Zabrze, Poland
autor
- Department of Biopharmacy, Medical University of Silesia, Sosnowiec, Poland
Bibliografia
- [1] Jayakrishnan A., Jameela S.R., Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices. Biomaterials 17 (1996) 417-84.
- [2] Gendler E., Gendler S., Nimni M.E. Toxic reactions evoked by glutaraldehyde-fixed pericardium and cardiac valve tissue bioprosthesis. J Biomed Mater Res. 18 (1984) 727-36.
- [3] Levy R.J., Schoen F.J., Anderson H.C., Harasaki H., Koch T.H., Brown W., Lian J.B., Cumming R., Gavin J.B. Cardiovascular implant calcification: a survey and update. Biomaterials 12 (1991) 707-14.
- [4] Cwalina B., Turek A., Nożyński J., Jastrzębska M., Nawrat Z. Structural changes in pericardium tissue modified with tannic acid. Int J Artif Organs. 28 (2005) 648-53.
- [5] Jastrzebska M., Barwiński B., Mróz I., Turek A., Zalewska-Rejdak J., Cwalina B. Atomic force microscopy investigation of chemically stabilized pericardium tissue. Eur Phys J E Soft Matter. 16 (2005) 381-8.
- [6] Jastrzebska M., Zalewska-Rejdak J., Wrzalik R., Kocot A., Mróz I., Barwiński B., Turek A., Cwalina B. Tannic acid-stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations. J Biomed Mater Res A. 78 (2006) 148-56.
- [7] Isenburg J.C., Simionescu D.T., Vyavahare N.R. Tannic acid treatment enhances biostability and reduces calcification of glutaraldehyde fixed aortic wall. Biomaterials 26 (2005) 1237-45.
- [8] Simionescu D., Simionescu A., Deac R. Detection of remnant proteolytic activities in unimplanted glutaraldehyde-treated bovine pericardium and explanted cardiac bioprostheses. J Biomed Mater Res. 27 (1993) 821-9.
- [9] Horcas I., Fernández R., Gómez-Rodríguez J.M., Colchero J., Gómez-Herrero J., Baro A.M. WSXM: a software for scanning probe microscopy and a tool for nanotechnology. Rev Sci Instrum. 78 (2007) 013705.
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Bibliografia
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