Composition of norfloxacin-resistant bacteria and isolation of norfloxacin-degrading bacteria in subtropical aquaculture ponds in China

• Autorzy: Mao Lutian , Chen Lifen , Wang Xirui , Xu Zhongbao , Ouyang Hui , Huang Biyou , Zhou Libin

Identyfikatory

DOI

10.24425/aep.2022.143712

• Języki publikacji: EN

Abstrakty

EN

To analyze the composition of norfloxacin-resistant bacteria and norfloxacin-degrading bacteria in pond water and sediment in subtropical China, the composition of antibiotic resistant bacteria in pond water and sediment enriched with norfloxacin-containing medium was analyzed by high-throughput sequencing. Sediment and water samples were collected from 3 fish ponds in subtropical China, and domesticated with norfloxacin, subsequently norfloxacin-resistant bacteria through high-throughput sequencing of 16S rDNA, and isolated norfloxacin--degrading bacteria. Our results showed that the pond sediment and water contain a variety of norfloxacin-resistant bacteria, mainly from Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, and Chloroflexi. Moreover, we isolated two norfloxacin-degrading bacteria (NorXu-2 and NorXu-3). The norfloxacin-degrading rate by NorXu-2 and NorXu-3 in the culture mediums with 200 μg/mL was the highest, which was up to 49.71% and 35.79%, respectively. When the norfloxacin concentration was 200 μg/mL, NorXu-2 and NorXu-3 had the best norfloxacin- -degrading effect at pH of 6, and the degradation rates were 53.64% and 45.54%, respectively. Moreover, NorXu-3 exhibited a good tolerance to high NaCl concentration. These results not only provided basic data for the follow-up study of the molecular mechanism of antimicrobial microbial degradation, but also provided potential norfloxacin degrading bacteria for norfloxacin removal and bioremediation in aquaculture environment.

Słowa kluczowe

EN

high-throughput sequencing; norfloxacin; antibiotic resistant bacterium; pond culture

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2022
• Tom: Vol. 48, no. 4
• Strony: 95--101
• Opis fizyczny: Bibliogr. 32 poz., rys., tab., wykr.

Twórcy

autor

Mao Lutian

• Huizhou University, Huizhou City, China

autor

Chen Lifen

• Huizhou University, Huizhou City, China

autor

Wang Xirui

• Huizhou University, Huizhou City, China

autor

Xu Zhongbao

• Huizhou University, Huizhou City, China

autor

Ouyang Hui

• Huizhou University, Huizhou City, China

autor

Huang Biyou

• Huizhou University, Huizhou City, China

autor

Zhou Libin

• Huizhou University, Huizhou City, China

Bibliografia

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Uwagi

PL

Opracowane ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023)