Photodynamic therapy procedures in the microfluidic system

• Autorzy: Jędrych E. , Pawlicka Z. , Chudy M. , Dybko A. , Brzózka Z.
• Języki publikacji: EN

Abstrakty

EN

Evaluation of the effi ciency of photodynamic therapy (PDT) in a hybrid microfl uidic culture system was studied. The geometry of the utilized microsystem for PDT procedures consists microchambers for cell culture and microchannels, which create a concentration gradient generator (CGG). 5-aminolevulinic acid (ALA) as a precursor of the photosensitizer was used. The geometry of the microchip allowed to test diff erent concentrations of ALA in a single assay. Evaluation of the effi ciency of photodynamic therapy was determined 24 hours after PDT procedure (irradiation with light which induced accumulated in carcinoma cells). The performed microsystem contained two independent micropatterns, that enables examination simultaneously various cell lines (carcinoma and normal) or various photosensitizers.

Słowa kluczowe

EN

photodynamic therapy (PDT); microfl uidic culture system; PDMS; adherent cell culture; concentration gradient generator (CGG); 5-aminolevulinic acid (ALA)

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2010
• Tom: Vol. 1, no. 1
• Strony: 30--33
• Opis fizyczny: Bibliogr. 5 poz., wykr., rys.

Twórcy

autor

Jędrych E.

• Department of Microbioanalytics, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw

autor

Pawlicka Z.

autor

Chudy M.

autor

Dybko A.

autor

Brzózka Z.

Bibliografia

• [1] Kudinova, N.V., and T.T. Berezov. “Photodynamic therapy of cancer: Search for ideal photosensitizer”. Biochemistry Supplemental Series B: Biomedical Chemistry 4 (2010): 95–103.
• [2] Friesen, S.A., et al. “5-Aminolevulinic acid-based photodynamic detection and therapy of brain tumors (review)”. Int J Oncol. 21 (2002): 577–582.
• [3] Betz, C.S., et al. “In vitro photodynamic therapy of nasopharyngeal carcinoma using 5-aminolevulinic acid”. Photochem Photobiol Sci. 1 (2002): 15–19.
• [4] Manivasager, V., et al. “A study comparing endogenous protoporphyrin IX induced by 5-ALA and ALA-methyl ester with exogenous PpIX and PpIX dimethyl ester in photodynamic diagnosis”. Int J Oncol. 29 (2006): 997–1002.
• [5] Sung, J.H., and M.L. Shuler. “In vitro microscale systems for systematic drug toxicity study”. Bioprocess Biosyst Eng. 33 (2010): 5–19.