Assessment of exposure to fungal aerosol in the lecture rooms of schools in the Lesser Poland region

• Autorzy: Frączek Krzysztof , Bulski Karol , Chmiel Maria

Identyfikatory

DOI

10.24425/aep.2023.148688

Warianty tytułu

PL

Ocena narażenia na aerozol grzybowy w pomieszczeniach szkół województwa małopolskiego

• Języki publikacji: EN

Abstrakty

EN

The paper presents an assessment of the mycological air quality in classrooms of school buildings located in Lesser Poland. In 10 schools, 5 sampling points were designated: 4 indoors and 1 as an "outdoor background". A 6-stage Andersen impactor was used to collect fungal aerosol samples. During sampling, dust measurements were made (using the DustTrak II dust meter) as well as temperature and relative humidity. The predominant genera of fungi were determined by the MALDI-TOF MS method. The results indicated no statistically significant differences in indoor air fungal concentrations among the tested locations (p>0.05). The highest concentrations were observed in large classrooms (max. 2,678 CFU∙m-3), however, these differences were not statistically significant across different types of school rooms (Kruskal-Wallis test: p>0.05). All rooms exhibited similar levels of fungal aerosol contamination. Relative air humidity had a significant influence on the number of microorganisms. The most frequently isolated fungi belonged to Cladosporium, Penicillium, and Aspergillus genera. Fungal aerosol concentrations in the tested classrooms did not exceed proposed limit values for this type of indoor environment. The results suggest that natural ventilation in classrooms is insufficient to ensure adequate microbiological quality of indoor air.

PL

Ocena jakości mykologicznej powietrza w pomieszczeniach dydaktycznych 10 budynków szkolnych zlokalizowanych w województwie małopolskim (Polska). Do badań aerozolu grzybowego wykorzystano 6-stopniowy impaktor kaskadowy Andersena. Podczas pobierania próbek wykonano pomiary zapylenia (za pomocą pyłomierza DustTrak II) oraz temperatury i wilgotności względnej. Badano powietrze w małych i dużych klasach oraz w salach gimnastycznych, tło wewnętrzne stanowiły korytarze a zewnętrzne powietrze atmosferyczne. Dominujące rodzaje grzybów oznaczono metodą MALDI-TOF MS. Na podstawie uzyskanych wyników stwierdzono, że pomiędzy badanymi obiektami (szkołami) nie wystąpiły statystycznie istotne różnice w stężeniu grzybów w powietrzu wewnętrznym (p>0,05). Największe zróżnicowanie aerozolu grzybowego obserwowano wdużych salach lekcyjnych (max. 2678 CFU∙m-3), jednak różnice te nie były istotne statystycznie pomiędzy różnymi typami pomieszczeń (test Kruskala-Wallisa: p>0,05). Wszystkie pomieszczenia były w porównywalnym stopniu zanieczyszczone aerozolem grzybowym. Najczęściej izolowane grzyby reprezentowały rodzaje Cladosporium, Penicillium i Aspergillus. Wilgotność względna powietrza miała istotny wpływ na liczebność mikroorganizmów. Stężenia aerozolu grzybowego w badanych pomieszczeniach dydaktycznych nie przekraczały proponowanych wartości granicznych dla tego typu wnętrz. Wyniki wskazują, że wentylacja naturalna w pomieszczeniach szkolnych nie wystarcza do zapewnienia odpowiedniej jakości mikrobiologicznej powietrza.

Słowa kluczowe

EN

air pollution; fungi; bioaerosol; schools; classroom

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2023
• Tom: Vol. 49, no 4
• Strony: 95--102
• Opis fizyczny: Bibliogr. 35 poz., tab., wykr.

Twórcy

autor

Frączek Krzysztof

• Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, Hugo Kołłątaj University of Agriculture, Krakow, Poland

autor

Bulski Karol

• Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, Hugo Kołłątaj University of Agriculture, Krakow, Poland

autor

Chmiel Maria

• Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, Hugo Kołłątaj University of Agriculture, Krakow, Poland

• https://orcid.org/0000-0003-0075-6337+

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).