Separation and analysis of phenylethanoid glycosides in Lamiophlomis rotata by high-performance liquid chromatography

• Autorzy: Dong S. , Shi X. , Liu Q. , Zhang Z. , Zhao L.
• Języki publikacji: EN

Abstrakty

EN

A simple hydrolysis method has been developed for determination of phenylethanoid glycosides in Lamiophlomis rotata (L.R.). Different kinds of phenylethanoid glycosides were hydrolyzed in hydrochloric acid solution to produce corresponding phenethyl alcohols and cinnamic acids, mainly containing hydroxytyrosol, homovanillyl alcohol, 3,4-dimethoxyphenethyl alcohol, caffeic acid, fumalic acid and 3,4-dimethoxycinnamic acid. The six analytes could be determined simultaneously by high-performance liquid chromatography (HPLC). The effects of mobile phase, pH and concentration of running buffer, detection wavelength, flow rate and injection volume were also investigated. Under the optimum conditions, the six hydrolyzates could be perfectly separated within 45 min. The response was linear over four orders of magnitude with detection limits (S/N = 3) ranging from 1 × 10 ^-8 to 1.5 × 10^-4 mol L^-1 for the analytes. The method has been successfully applied to the analysis of real sample Du-Yi-Wei capsule and Qi-Zheng-Yan-Tong patch, with satisfactory results.

Słowa kluczowe

EN

Lamiophlomis rotata (Benth) Kudo; high-performance liquid chromatography; phenylethanoid glycosides; hydrolyzation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 2
• Strony: 377--392
• Opis fizyczny: Bibliogr. 30 poz., rys., tab.

Twórcy

autor

Dong S.

• The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China

autor

Shi X.

• Gansu Academy of Medical Sciences Lanzhou 730000 China

autor

Liu Q.

• Graduate University of the Chinese Academy of Sciences Beijing 100039 China

autor

Zhang Z.

• The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China
• Graduate University of the Chinese Academy of Sciences Beijing 100039 China

autor

Zhao L.

• The Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics Lanzhou 730000 China

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