Microbiological Landscape of Oil-Contaminated Soil and its Bioremediation by Microorganisms

• Autorzy: Kupriyashkina Elena , Pylypenko Liudmyla , Sevastyanova Olena , Mazurenko Kseniya , Hugi Christoph , Breitenmoser Lena , Krusir Galyna , Malyovanyi Myroslav

Identyfikatory

DOI

10.12912/27197050/175139

• Języki publikacji: EN

Abstrakty

EN

The composition of microbial contaminants of soil samples polluted with oil and oil products from oil depots of ports in southern Ukraine was investigated, and the possibility of their bioremediation by microorganisms present in the soil was determined. The microbiological landscape of the soil contaminated with oil and oil products was established, the quantitative and qualitative characteristics, group and dendrological composition of microorganisms as well as their potential ability to biodegrade petroleum hydrocarbons were determined. The degree of sanitary and ecological contamination of the samples was characterized by the number of the main groups of microorganisms – mesophilic aerobic and facultative anaerobic microorganisms (MAFAnM), molds, yeasts, as well as the dominance of MAFAnM by 3-5 orders among the studied groups of microorganisms. According to MAFANM, the number of thermophilic bacteria, titers of nitrifying bacteria, E. coli, Clostridium perfringens, bacteria of the genus Proteus, and the degree of oil contamination, the soil samples studied are characterized as contaminated and heavily contaminated. According to the study of morphological, tintorial, cultural, biochemical properties, 130 species were identified and 9 morphogroups of bacteria in oil-contaminated soil samples were determined. A dendrogram was constructed based on the set of studied properties of the isolated microorganisms. According to the results of the screening, the microorganisms isolated from the contaminated soil samples are capable of biodegradation of long-chain alkanes of petroleum hydrocarbons. The identified groups of microorganisms can be arranged in the following order of increasing indicator: Bacillus subtilis and Paenibacillus macerans ˂ Paenibacillus polymyxa ˂ Bacillus licheniformis ˂ Bacillus thuringiensis ˂ Bacillus megaterium ˂ Bacillus pumilis ˂ Bacillus cereus ˂ Paenibacillus circulans. Paenibacillus circulans and Bacillus cereus were identified as the most promising strains, biotransforming up to 48 percent of the total amount of hydrocarbons.

Słowa kluczowe

EN

oil-contaminated soil; ecological biotechnology; biodegradation; microorganism; petroleum hydrocarbons

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2024
• Tom: Vol. 25, iss. 2
• Strony: 31--40
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Kupriyashkina Elena

• Odesa National University of Technology, 112 Kanatna Str,, Odessa, 65039, Ukraine

• https://orcid.org/0009-0005-4207-6024

autor

Pylypenko Liudmyla

• Odesa National University of Technology, 112 Kanatna Str,, Odessa, 65039, Ukraine

autor

Sevastyanova Olena

• Odesa National University of Technology, 112 Kanatna Str,, Odessa, 65039, Ukraine

autor

Mazurenko Kseniya

• Odesa National University of Technology, 112 Kanatna Str,, Odessa, 65039, Ukraine

autor

Hugi Christoph

• Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciencesund Arts Northwestern Switzerland, Hofackerstrasse 30, 4132 Muttenz, Switzerland

autor

Breitenmoser Lena

• Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciencesund Arts Northwestern Switzerland, Hofackerstrasse 30, 4132 Muttenz, Switzerland

autor

Krusir Galyna

• Odesa National University of Technology, 112 Kanatna Str,, Odessa, 65039, Ukraine
• Institute for Ecopreneurship, School of Life Sciences, University of Applied Sciencesund Arts Northwestern Switzerland, Hofackerstrasse 30, 4132 Muttenz, Switzerland

• https://orcid.org/0000-0001-6464-5754

autor

Malyovanyi Myroslav

• National University "Lviv Polytechnic", 12 Stepan Bandera Str., Lviv, 79000, Ukraine

• https://orcid.org/0000-0002-3868-1070

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).