A simplified and small-scale sample preparation technique for determining astaxanthin, canthaxanthin, and β-apo-8′-carotenoic acid ethyl ester in hen’s egg yolk

• Autorzy: Furusawa N.
• Języki publikacji: EN

Abstrakty

EN

This paper described a fast, simple, and small scale method of sample preparation followed by high-performance liquid chromatography (HPLC) coupled photodiode array (PDA) detector for simultaneous quantification of astaxanthin, canthaxanthin, and β-apo-8′-carotenoic acid ethyl ester in hen’s egg yolk. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analytes were extracted from the sample using a handheld ultrasonic homogenizer, and purified by MonoSpin®-SI, a centrifugal monolithic silica spin mini-columns, and quantified within 30 min. The proposed method obtained average recoveries for the three analytes in the range of 70.5–101.1% with relative standard deviations ≤5.0%. The quantitation limits were 0.3 μg g ^-1 for AX, 0.5 μg/g for CX, and 1.0 μg g−1 for ACAEE, respectively.

Słowa kluczowe

EN

astaxanthin; canthaxanthin; β-apo-8′-carotenoic acid ethyl ester; centrifugal monolithic silica spin mini-column

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 4
• Strony: 587--597
• Opis fizyczny: Bibliogr. 28 poz., rys., tab.

Twórcy

autor

Furusawa N.

• Osaka City University Graduate School of Human Life Science Osaka 558-8585 Japan

Bibliografia

• [1] European Food Safety Authority (EFSA), EFSA J., 291, 1 (2005)
• [2] EFSA, EFSA J., 507, 1 (2007)
• [3] The Natural Food Colour Association (published by webmaster on Fri, 04/29/2011), The legislation of food colours in Europe ( http://www.natcol.org/node/5#13b )
• [4] Japanese Ministry of Agriculture, Forestry and Fisheries law 40th ( http://law.egov.go.jp/htmldata/S51/S51F00601000035.html )
• [5] DSM Nutritional Products, DSM Guidelines for egg yolk pigmentation with CAROPHYLL® ( http://www.christa.bg/files/Catalogue/105.pdf )
• [6] Lonye Technology Limited ( http://www.lonyetech.com/ProductsView.asp?id=136 )
• [7] J.C. Na, J.Y. Song, B.D. Lee, S.J. Lee, C.Y. Lee, and G.H. An, Anim. Feed Sci. Technol., 117, 305 (2004)
• [8] F. Sirri, N. Iaffaldano, G. Minelli, A. Meluzzi, M.P. Rosato, and A. Franchini, J. Appl. Poult. Res., 16, 429 (2007)
• [9] E. Santos-Bocanegra, X. Ospina-Osorio, and E.O. Oviedo-Rondón, Int. J. Poult. Sci., 3, 685 (2004)
• [10] Algae to Omega Holdings, Astaxanthin ( http://www.algae2omega.com/astaxanthin )
• [11] Nichiwa Sangyo Co., Ltd., Patentjp.com. ( http://www.patentjp.com/07/A/A100829/DA10002.html )
• [12] Mercian Corporation, Patentjp.com. ( http://www.patentjp.com/09/L/L100272/DA10001.html )
• [13] Uehara Yokei ( http://tamago-uehara.com/info.html )
• [14] Astaxanthin ( http://www.astaxanthine.info/2009/03/post_23.html )
• [15] EFSA, EFSA J., 1852, 1 (2010) ( http://www.efsa.europa.eu/en/efsajournal/pub/1852.htm )
• [16] EFSA, EFSA J., 1095, 1 (2009) ( http://www.efsa.europa.eu/en/efsajournal/pub/1098.htm )
• [17] The Japan Food Chemical Research Foundation (JFCRF), 2009. ( http://www.m5.ws001.squarestart.ne.jp/foundation/fooddtl.php?f_inq=19600 )
• [18] J. Schlatterer and D.E. Breithaupt, J. Agri. Food Chem., 54, 2267 (2006)
• [19] J. Rajesha, B. Madhusudhan, M.M. Swamy, R.J. Rao, G.A. Ravishankar, A. Rangarao, and M. Karunakumar, J. Food Sci. Technol. (Mysore), 46, 509 (2009)
• [20] M. Wenzel, I. Seuss-Baum, and E. Schlich, J. Agr. Food Chem., 58, 1726 (2010)
• [21] Furusawa, N., Food Chem., 124, 1643 (2011)
• [22] FDA/CDER/CVM, Guidelines for Industry—Bioanalytical Method Validation, 2001. ( http://www.fda.gov/downloads/Drugs/GuidanceComplianceRegulatory-Information/Guidances/UCM070107.pdf )
• [23] L. Huber, Validation and Quantification in Analytical Laboratories, Interpharm Press, East England, CO, USA, 1998
• [24] ICH, Work Products, ICH Guidelines, Quality Guidelines ( http://www.ich.org/products/guidelines.html )
• [25] AOAC International, Method Validation, Guidelines & References ( http://www.aoac.org/vmeth/guidelines.htm )
• [26] Codex alimentarius commission, ALINORM 01/23, Codex Committee on Methods of Analysis and Sampling, Joint FAO/WHO Food Standards Programme, the 23rd Section of the Codex Committee on Method of Analysis and Sampling ( http://www.codexalimentarius.net/web/archives.jsp?year=01 )
• [27] GL Sciences, Monolithic SPE Column for the Purification and Enrichment of Small Amount Sample, MonoSpin® Series ( http://www.glsciences.com/products/monolithic_products/mono_spin.pdf )
• [28] EU Decision 2002/657, Official Journal European Communication L221/8