Development and validation of a reversed-phase HPLC method for simultaneous determination of doxazosin mesylate and finasteride

• Autorzy: Krotz D. , Mengesha A. E.

Identyfikatory

DOI

10.1556/1326.2017.29.3.01

• Języki publikacji: EN

Abstrakty

EN

A reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed and validated for the simultaneous determination of doxazosin mesylate (DOX) and finasteride (FIN) in bulk powders and pharmaceutical formulations. The compounds were separated on a Pinnacle II C18 column (250 × 4.6 mm i.d.; particle size, 5 μm) with an isocratic mobile phase at a flow rate of 1.0 mL min−1. The mobile phase was a mixture of 25 mM ammonium acetate and acetonitrile in the ratio of 50:50 %v/v. The pH of the buffer was adjusted to 4.0 ± 0.05 with glacial acetic acid. The detection was performed at 230 nm. The total chromatographic analysis time per sample was 15 min with DOX and FIN eluting at 3.9 and 7.2 min, respectively. The accuracy, precision, specificity, linearity, and sensitivity of the method were validated according to the International Conference on Harmonization (ICH) guidelines. The calibration plots were linear (r2 > 0.999) over the concentration range 24.25–291.0 μg mL−1 and 122.5–1470.0 μg mL−1 for DOX and FIN, respectively. The method was used for the simultaneous determination of DOX and FIN in capsules.

Słowa kluczowe

EN

doxazosin mesylate; finasteride; RP-HPLC; method validation; simultaneous analysis

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2017
• Tom: Vol. 29, no. 3
• Strony: 309--324
• Opis fizyczny: Bibliogr. 25 poz., rys.

Twórcy

autor

Krotz D.

• College of Arts and Sciences, Drake University, Des Moines, IA, USA

autor

Mengesha A. E.

• College of Pharmacy and Health Sciences, Drake University, Des Moines, IA, USA

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).