Actinobacterial Communities of Chosen Extreme Habitats in China

• Autorzy: Jia Pengli , Li Miao , Feng Haiyan , Ma Mutian , Gai Jingping , Yang Zhongfang

Identyfikatory

DOI

10.3161/15052249PJE2020.68.3.001

• Języki publikacji: EN

Abstrakty

EN

Actinobacteria living in frozen soils which potentially could be producers of valuable and biologically active metabolites, remain still largely unexplored. Therefore, the diversity of culturable actinobacteria in the six frozen soil samples collected from the high-latitude and the high-altitude regions in China was investigated by using the culture-dependent method and 16S rRNA gene analysis. A total of 184 culturable actinobacterial isolates (morphotypes) were obtained. The abundance of culturable actinobacteria was 6.20 × 10³–3.49 × 10⁵ colony forming units (CFU) per gram of soil (dry weight). The actinobacteria isolated from the high-latitude region in China belong to five genera: Streptacidiphilus, Kitasatospora, Streptomyces, Arthrobacter, and Rhodococcus. The strains belonging to Arthrobacter were dominant, the 19 strains were closely related to Arthrobacter globiformis in terms of evolutionary distance. The actinobacterial isolates from the high-altitude region, from 2,813 m to 4,775 m in elevation, in China could be grouped into six genera (Arthrobacter, Microbacterium, Streptomyces, Rhodococcus, Nocardia, and Nocardiopsis), with dominant Streptomyces and Arthrobacter strains. The 4 strains were closely related to Rhodococcus fascians and one strain was closely with Microbacterium hydrocarbonoxydans in terms of evolutionary distance. Three genera of Arthrobacter, Streptomyces, and Rhodococcus were found in both areas. Our research provides new insight into the characteristics of the distribution of actinobacteria associated with frozen soils, which help us gain a better understanding of the potential of the cryogenic environments – the potential source of actinobacterial antibiotics.

Słowa kluczowe

EN

16S rRNA; actinobacteria; frozen soils; Greater Khingan Range; Tibetan plateau

PL

16S rRNA; promieniowce; zamarznięte gleby; Wielki Chingan; Wyżyna Tybetańska

• Wydawca: Museum and Institute of Zoology, Polish Academy of Sciences
• Czasopismo: Polish Journal of Ecology
• Rocznik: 2020
• Tom: Vol. 68, nr 3
• Strony: 181--194
• Opis fizyczny: Bibliogr. 66 poz., rys., tab., wykr.

Twórcy

autor

Jia Pengli

• School of Earth Sciences and Resources, China University of Geosciences, 29 Xueyuan Road, Beijing 100083, China

autor

Li Miao

• School of Earth Sciences and Resources, China University of Geosciences, 29 Xueyuan Road, Beijing 100083, China

autor

Feng Haiyan

• School of Earth Sciences and Resources, China University of Geosciences, 29 Xueyuan Road, Beijing 100083, China

autor

Ma Mutian

• School of Earth Sciences and Resources, China University of Geosciences, 29 Xueyuan Road, Beijing 100083, China

autor

Gai Jingping

• College of Resources and Environmental Sciences, China Agricultural University, Key Laboratory of Plant-Soil Interactions, Ministry of Education, 2 Yuanmingyuan West Road, Beijing 100193, China

autor

Yang Zhongfang

• School of Earth Sciences and Resources, China University of Geosciences, 29 Xueyuan Road, Beijing 100083, China

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