The influence of autologous and heterologous extract of antimicrobial peptides on leukocytes isolated during titanium implant insertion in rabbit and ovine model

• Autorzy: Zdziennicka Joanna , Szponder Tomasz , Latalski Michał , Żylińska Beata , Sobczyńska-Rak Aleksandra , Wessely-Szponder Joanna

Identyfikatory

DOI

10.34821/eng.biomat.159.2021.24-30

• Języki publikacji: EN

Abstrakty

EN

This study evaluated the in vitro leukocyte response to titanium implants in the presence of autologous or heterologous antimicrobial peptides extracts. Antimicrobial peptides (AMPs) appeared to be a new approach both against microorganisms and for regulation of inflammatory and repair processes. To evaluate their potential usefulness in regenerative medicine, we prepared different extracts of neutrophil-derived AMPs from rabbit, ovine or porcine blood which contained AMPs of different compositions, mainly defensins, cathelicidins and fragments thereof. Then, we assessed in vitro the influence of different AMPs extracts on the neutrophils and monocyte-derived macrophages (MDM) activity. For this purpose, these cells were obtained from experimental animals, rabbits, or sheep submitted to insertion of a titanium implant into the tibial defect. The cultured cells stimulation was autologous or heterologous, dependently on the AMPs extract origin and the experimental animal species. The neutrophil activity was assessed on the basis of the enzymes release from azurophilic and secondary granules and the free radicals generation. The MDM functional assessment was based on the NO and superoxide generation and arginase activity. Additionally, morphological changes were evaluated in the cell cultures. Our results indicated that the origin of AMPs extract is crucial for its activity. The autologous extracts stimulated anti-inflammatory responses, whereas the heterologous extracts displayed pro-inflammatory effect on neutrophils and macrophages. These results might be considered during the introduction of new preparations in regenerative medicine.

Słowa kluczowe

EN

titanium implants; neutrophil extract; implantation of biomaterial; neutrophils; monocyte- -derived macrophages; natural antimicrobial peptides

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2021
• Tom: Vol. 24, no. 159
• Strony: 24--30
• Opis fizyczny: Bibliogr. 19 poz., rys.

Twórcy

autor

Zdziennicka Joanna

• Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland

autor

Szponder Tomasz

• Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

autor

Latalski Michał

• Department of Pediatric Orthopedics, Medical University, Gębali 6, 20-093 Lublin, Poland

autor

Żylińska Beata

• Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

autor

Sobczyńska-Rak Aleksandra

• Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland

autor

Wessely-Szponder Joanna

• Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-033 Lublin, Poland

Bibliografia

• [1] Frisken K.W., Dandie G.W., Lugowski S., Jordan G.: A Study of Titanium Release into Body Organs Following the Insertion of Single Threaded Screw Implants into the Mandibles of Sheep. Australian Dental Journal 47(3) (2002) 214-217.
• [2] Pettersson M., Pettersson J., Thorén M.M., Johansson A.: Effect of cobalt ions on the interaction between macrophages and titanium. Journal of Biomedical Materials Research. Part A 106(9) (2018) 2518-2530.
• [3] Novak M.L., Koh T.J.: Macrophage phenotypes during tissue repair. Journal of Leukocyte Biology 93 (2013) 875-881.
• [4] Wachi T., Shuto T., Shinohara Y., Matono Y., Makihira S.: Release of titanium ions from an implant surface and their effect on cytokine production related to alveolar bone resorption. Toxicology 327 (2015) 1-6.
• [5] Pettersson M., Kelk P., Belibasakis G.N., Bylund D., Thorén M.M., Johansson A.: Titanium ions form particles that activate and execute interleukin-1b release from lipopolysaccharide-primed macrophages. Journal of Periodontal Research 52(1) (2017) 21–32.
• [6] Pettersson M., Pettersson J., Thorén M.M., Johansson A.: Release of titanium after insertion of dental implants with different surface characteristics – an ex vivo animal study. Acta Biomaterialia Odontologica Scandinavica 3(1) (2017) 63-73.
• [7] Pettersson M., Pettersson J., Johansson A., Thorén M.M.: Titanium release in peri-implantitis. Journal of Oral Rehabilitation 46(2) (2019) 179–188.
• [8] Perobelli S.M., Silva T.G., Bonomo A.: Neutrophils Plasticity: The Regulatory Interface in Various Pathological Conditions. Role of Neutrophils in Disease Pathogenesis, Intech, Chapter 7, 2017.
• [9] Mansour S.C., Pena O.M., Hancock R.: Host defense peptides: front-line immunomodulators. Trends in Immunology 35 (2014) 443-450.
• [10] Kumar R., Ali S.A., Singh S.K., Bhushan V., Mathur M., Jamwal S., Mohanty A.K., Kaushik J.K., Kumar S.: Antimicrobial Peptides in Farm Animals: An Updated Review on Its Diversity, Function, Modes of Action and Therapeutic Prospects. Veterinary Sciences 7 (2020) 206.
• [11] Haiwen Z., Rui H., Bingxi Z., Qingfeng G., Beibei W., Jifeng Z., Xuemei W., Kebang W.: Cathelicidin- derived PR39 protects enterohemorrhagic Escherichia coli O157:H7 challenged mice by improving epithelial function and balancing the microbiota in the intestine. Scientific Reports. 9:9456 (2019).
• [12] Mookherjee N., Anderson M.A., Haagsman H.P., Davidson D.J.: Antimicrobial host defence peptides: functions and clinical potential. Nature Reviews Drug Discovery 19 (2020) 311–332.
• [13] Pokrowiecki R., Szaraniec B., Zaręba T., Szponder T., Pałka K., Chłopek J., Tyski S.: Titanium modified with silver nanoparticles for oral implantology. In: 27th European Conference on Biomaterials ESB, Krakow, Poland, Proceedings (2015) pp 64.
• [14] Wessely-Szponder J., Szponder T., Bobowiec R.: Different activation of monocyte-derived macrophages by antimicrobial peptides at a titanium tibial implantation in rabbits. Research in Veterinary Science 115 (2017) 201-210.
• [15] Szponder T., Wessely-Szponder J., Smolira A.: Evaluation of platelet-rich plasma and neutrophil antimicrobial extract as two autologous blood-derived agents. Tissue Engineering and Regenerative Medicine 14(3) (2017) 287-296.
• [16] Campbell L., Saville C.R., Murray P.J., Cruickshank S.M., Hardman M.J.: Local arginase 1 activity is required for cutaneous wound healing. The Journal of investigative dermatology 133(10) (2013) 2461-2470.
• [17] Moura C.C., Zanetta-Barbosa D., Dechichi P., Carvalho V.F., Fereira Soares P.: Effects of titanium surfaces on the development profile of monocytes/macrophages. Brasilian Dental Journal 25 (2014) 96-103.
• [18] Wessely-Szponder J., Zdziennicka J., Szponder T., Latalski M., Żylińska B., Sobczyńska-Rak A.: The effect of autologous and heterologous antimicrobial peptides extract on rabbit and ovine leukocytes evaluated during repair process. Published: 19 April 2021 by MDPI in The 1st International Electronic Conference on Antibiotics—The Equal Power of Antibiotics And Antimicrobial Resistance session Antimicrobial Peptides 10.3390/ECA2021-09601 (registering DOI).
• [19] Douglas T.E.L., Hempel U., Zydek J., Vladescu A., Pietryga K., Kaeswurm J.A.H., Buchweitz M., Surmenev R.A., Surmeneva M.A., Cotrutf C.M., Koptyug A.V., Pamuła E.: Pectin coatings on titanium alloy scaffolds produced by additive manufacturing: Promotion of human bone marrow stromal cell proliferation. Materials Letters 227 (2018) 225–228

Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).