Pozaustrojowe badania nad toksycznym oddziaływaniem kopolimeru PLGA z dodatkiem hydroksyapatytu na ludzkie osteoblasty linii hFOB 1.19

• Autorzy: Cieślik M. , Mertas A. , Morawska-Chochół A. , Orlicki R. , Owczarek A. , Król W.

Warianty tytułu

EN

In vitro examinations of toxic influence of PLGA co-polymer mixed with hydroxyapatite upon human osteoblasts line hFOB 1.19

• Języki publikacji: PL EN

Abstrakty

PL

W celu biologicznej oceny w warunkach in vitro kopolimeru glikolidu z laktydem z dodatkiem hydroksyapatytu (PLGA+HA) dokonano oceny stopnia jego cytotoksyczności względem ludzkich osteoblastów linii hFOB 1.19. Wykonano pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH) po 24 i 48 godzinach kontaktu komórek z ekstraktem uzyskanym poprzez 8-dniową inkubację kompozytu w medium do hodowli osteoblastów. Kontaktowano ponadto badany materiał bezpośrednio z komórkami kościotwórczymi, a stopień cytotoksyczności oceniano po 24, 48 i 72 godzinach stosując w tym celu test LDH. W obu metodach badany materiał nie wpływał w sposób toksyczny na ludzkie osteoblasty.

EN

In order to evaluate lactide-glycolide co-polymer with admixture of hydroxyapatite (PLGA+HA) from biological point of view in vitro conditions, its level of toxicity for human osteoblasts line hFOB 1.19 was assessed. The activity of mitochondrial dehydrogenase and lactate dehydrogenase was measured (MTT test and LDH test respectively) after 24 and 48 hours of the cells’ contact with the extract obtained through 8-day incubation of the composite in osteoblasts cultivation medium. Apart from that the material examined was contacted with bone-forming cells and its degree of toxicity was assessed after 24, 48 and 72 hours using LDH test. In both methods the material under examination did not have any toxic influence upon human osteoblasts.

Słowa kluczowe

PL

kompozyt; kopolimer glikolidu z laktydem; hydroksyapatyt; cytotoksyczność; test MTT; test LDH; osteoblasty ludzkie; badania in vitro

EN

composite; lactide-glycolide co-polymer; hydroxyapatite; cytotoxicity; MTT test; LDH test; human osteoblasts; in vitro examinations

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2009
• Tom: Vol. 12, no. 89-91
• Strony: 97--102
• Opis fizyczny: Bibliogr. 27 poz., tab.

Twórcy

autor

Cieślik M.

• Śląski Uniwersytet Medyczny w Katowicach, Katedra i Zakład Materiałoznawstwa Stomatologicznego w Bytomiu

autor

Mertas A.

• Śląski Uniwersytet Medyczny w Katowicach Katedra i Zakład Mikrobiologii i Immunologii W Zabrzu

autor

Morawska-Chochół A.

• AGH, Wydział Inżynierii Materiałowej i Ceramiki, Katedra Biomateriałów, Kraków

autor

Orlicki R.

• Śląski Uniwersytet Medyczny w Katowicach, Katedra i Zakład Materiałoznawstwa Stomatologicznego W Bytomiu

autor

Owczarek A.

• Śląski Uniwersytet Medyczny w Katowicach Zakład Statystyki w Sosnowcu

autor

Król W.

• Śląski Uniwersytet Medyczny w Katowicach Katedra i Zakład Mikrobiologii i Immunologii w Zabrzu

Bibliografia

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