Application of modified silica gel in the process of trypsin immobilization

• Autorzy: Miłek Justyna , Kwiatkowska-Marks Sylwia , Trawczyńska Ilona
• Języki publikacji: EN

Abstrakty

EN

The paper presents the use of modified silica gel for the production of immobilized trypsin from bovine pancreas. Silica gel was modified with 3-aminopropyltriethoxysilane, followed by glutaraldehyde. The influence of stirring time on activity of the prepared biocatalyst was determined for individual stages of the modification. Activity of both native and immobilized trypsin was measured using Kunitz method. At the temperature of 55℃ and pH 7.6 native and immobilized trypsin onto modified silica gel indicate optimum activity. The influence of multiple recycling and storage time on activity of immobilized trypsin was tested. After fourteen days of storage at the temperature of 4℃ immobilized trypsin exhibits 75% of its initial activity.

Słowa kluczowe

EN

trypsin; immobilization; silica gel; biocatalyst

• Wydawca: Wydawnictwo Uniwersytetu Warmińsko-Mazurskiego w Olsztynie
• Czasopismo: Technical Sciences / University of Warmia and Mazury in Olsztyn
• Rocznik: 2019
• Tom: nr 22(1)
• Strony: 35--43
• Opis fizyczny: Bibliogr. 12 poz., rys., tab., wykr.

Twórcy

autor

Miłek Justyna

• Zakład Inżynierii Chemicznej i Bioprocesowej, Wydział Technologii i Inżynierii Chemicznej, Uniwersytet Technologiczno-Przyrodniczy im. Jana i Jędrzeja Śniadeckich, ul. Seminaryjna 3, 85-326 Bydgoszcz

autor

Kwiatkowska-Marks Sylwia

• Department of Chemical and Bioprocess Engineering Faculty of Chemical Technology and Engineering University of Technology and Life Sciences

autor

Trawczyńska Ilona

• Department of Chemical and Bioprocess Engineering Faculty of Chemical Technology and Engineering University of Technology and Life Sciences

Bibliografia

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