Graphene oxide flake activation via divinylsulfone – a procedure for efficient β-galactosidase immobilization

• Autorzy: Trusek Anna

Identyfikatory

DOI

10.2478/pjct-2019-0006

• Języki publikacji: EN

Abstrakty

EN

Flaky graphene oxide was activated with divinylsulfone followed by immobilization of the β-galactosidase enzyme. An active and stable preparation was obtained. β-galactosidase stability after immobilization was much higher than with the native enzyme. The half-life time of the immobilized enzyme was estimated as 165 hours, while for the native form, the estimate was only 5 hours. The developed procedure for the preparation of flaked graphene and its use in the chemical immobilization of enzymes can be used for any enzyme. A processing solution for continuous operation was proposed and verified using cow’s milk, with lactose as the hydrolysed substrate, as a dosing stream. Lactose, a milk sugar, was effectively hydrolysed. Product for allergy sufferers who cannot digest lactose has been obtained in this way.

Słowa kluczowe

EN

graphene oxide; enzyme chemical immobilization; divinylsulfone; β-galactosidase; lactose-free product

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2019
• Tom: Vol. 21, nr 1
• Strony: 27--32
• Opis fizyczny: Bibliogr. 22 poz., rys., tab.

Twórcy

autor

Trusek Anna

• Wroclaw University of Science and Technology, Department of Bioprocess and Biomedical Engineering, Norwida 4/6, 50-373 Wroclaw, Poland

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).

Brak poz. 15 bibliografii.