Simultaneous reverse-phase HPLC determination of major antioxidant phenolics in Commelina benghalensis L. tubers

• Autorzy: Misra A. , Srivastava A. , Srivastava S. , Rawat A. K. S.
• Języki publikacji: EN

Abstrakty

EN

Commelina benghalensis (Commelinaceae) is widely used as traditional and folklore medicine in India. In the present study, a reverse-phase high-performance liquid chromatography—photodiode array detection (RP-HPLC—PDA) method was developed for the separation, identification, and quantification of bioactive phenolics. Antioxidant potential was also accessed to validate the presence of identified markers. Method was developed on C18 column with 1% formic acid (in water) and acetonitrile as solvent system, and data acquisitions were achieved at wavelength of 285 nm. The developed method was also validated for accuracy, precision, robustness, limit of detection and quantification (LOD and LOQ), repeatability, and recovery according to International Conference on Harmonization (ICH) guidelines. In this method, five phenolics, viz., protocatechuic acid (0.033%), vanillic acid (0.262%), ferulic acid (0.365%), apigenin (0.126%), and kaempferol (0.544%), were quantified in linearity range of 0.2–1.0 μg with correlation coefficient of more than 0.9949. Relative standard deviation (RSD) (%), LOD, LOQ, and recovery (%) are within the acceptable limit. Besides that, methanolic extract shows the inhibition (%) range from 24.45 to 68.75% at 0.02–0.12 mg Ml^-1. IC50 of extract was observed at 46.75 μg Ml^-1, suggesting the promising activity in methanol extract. Hence, the proposed method for simultaneous quantification of five bioactive phenolics in the tuber of C. benghalensis using HPLC–PDA detection under the specified conditions is specific and accurate, and validation proves its selectivity and reproducibility.

Słowa kluczowe

EN

reversed-phase HPLC; Commelina benghalensis; phenolics; antioxidant

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 4
• Strony: 541--554
• Opis fizyczny: Bibliogr. 14 poz., rys., tab.

Twórcy

autor

Misra A.

• Pharmacognosy and Ethanopharmacology Division, CSIR—National Botanical Research Institute, Lucknow-226001, India

autor

Srivastava A.

• Pharmacognosy and Ethanopharmacology Division, CSIR—National Botanical Research Institute, Lucknow-226001, India

autor

Srivastava S.

• Pharmacognosy and Ethanopharmacology Division, CSIR—National Botanical Research Institute, Lucknow-226001, India

autor

Rawat A. K. S.

• Pharmacognosy and Ethanopharmacology Division, CSIR—National Botanical Research Institute, Lucknow-226001, India

Bibliografia

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• [10] D. Hong and R. A. DeFillipps, Commelina diffusa. In: Z.Y. Wu, P.H. Raven, D.Y. Hong (Eds.) Flora of China, Science Press, Beijing; Missouri Botanical Garden Press, St. Louis, 2000, pp. 86
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• [13] ICH Guideline Q2R1, Validation of Analytical Procedures: Text and Methodology, Geneva, Switzerland, November, 2005, http://www.ich.org
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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.