Experimental and analytical procedures for the ToF-SIMS measurement data of membranous structures

• Autorzy: Skalska Magdalena E. , Durak-Kozica Martyna

Identyfikatory

DOI

10.5604/01.3001.0054.1935

• Języki publikacji: EN

Abstrakty

EN

Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful analytical technique with great application potential in biomolecular matter research. SIMS measurements performed on biological samples, due to their complex structure and the content of many small and large atomic molecular compounds, suffer very rich and complex mass spectra of particles, which characterise the content and physio-chemical properties of examined samples. The proper description and understanding of features appearing in the spectra and, consequently, the final data confirming or rejecting the hypothesis put forward in the experiment, largely depend on the experimenter’s correct understanding of the technique itself and its limitations, knowledge of the tested material and its appropriate preparation. These issues mean that obtaining the right answer to the questions posed in the research hypothesis requires not only the correct conduct of experiments but also the appropriate processing of post-experimental data. This study aims to demonstrate the impact of various analytical and experimental procedures applied to reach proper conclusions from TOF-SIM measurements. These are different types of data normalization, the selection of a so-called region of interest (ROI), the selection of representative secondary ions and specific quantification methods, including a combination of experimental parameters. All these aspects were checked and discussed based on the results of the analysis of pancreatic β cells placed in a PBS solution on silicon wafers.

Słowa kluczowe

EN

ToF-SIMS; membranous structures; data preparation; data acquisition; secondary ion mass spectrometry experiments

• Wydawca: Uniwersytet Jagielloński - Collegium Medicum ; Index Copernicus Sp. z o.o.
• Czasopismo: Bio-Algorithms and Med-Systems
• Rocznik: 2023
• Tom: Vol. 19, no. 1
• Strony: 64--68
• Opis fizyczny: Bibliogr. 12 poz., rys.

Twórcy

autor

Skalska Magdalena E.

• Department of Medical Physics, M. Smoluchowski Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland
• Centre for Theranostics, Jagiellonian University, Krakow, Poland

• https://orcid.org/0000-0003-0069-0550

autor

Durak-Kozica Martyna

• Department of Medical Physics, M. Smoluchowski Institute of Physics, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, Krakow, Poland
• Centre for Theranostics, Jagiellonian University, Krakow, Poland

• https://orcid.org/0000-0002-5275-6462

Bibliografia

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• 5. Marzec ME, Wojtysiak D, Połtowicz K, Nowak J, Pedrys R. Study of cholesterol and vitamin E levels in broiler meat from different feeding regimens by TOF-SIMS. Biointerphases 2016;11:02A326.
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• 7. Touboul D, Brunelle A, Halgand F, De La Porte S, Laprévote O. Lipid imaging by gold cluster time-of-flight secondary ion mass spectrometry: Application to Duchenne muscular dystrophy. J. Lipid Res. 2005;46:1388-95.
• 8. Wu L, Felton JS, Wu KJJ. Applying imaging ToF-SIMS and PCA in differentiation of tissue types. Methods Mol. Biol. 2010;656:267-81.
• 9. Debois D, Bralet M-P, Le Naour F, Brunelle A, Laprevote O. In situ lipidomic analysis of nonalcoholic fatty liver by cluster TOF-SIMS imaging. Anal. Chem. 2009;81:2823-31.
• 10. Magnusson Y, Friberg P, Sjövall P, Dangardt F, Malmberg P, Chen Y. Lipid imaging of human skeletal muscle using TOF-SIMS with bismuth cluster ion as a primary ion source. Clin. Physiol. Funct. Imaging 2008;28:202-9.
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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).