HPLC, Two-Dimensional TLC Determination of Phenolic Content, and an In Vitro Perspective to Antioxidant Potential of Euonymus verrucosus Scop. Extracts

• Autorzy: Kukula-Koch W. , Widelski J. , Koch W. , Głowniak K.
• Języki publikacji: EN

Abstrakty

EN

The presence of phenolic content in overground extracts of Euonymus verrucosus Scop. — commonly growing in Europe — has been reported recently. The chromatographical and spectral data revealed the presence of several simple phenolic acids (gallic, protocatechuic, p-hydroxybenzoic, vanillic, syringic, caffeic, p-coumaric, ferulic, and m-coumaric acids), both as free and conjugated with other secondary metabolites. The comparison of two-dimensional TLC systems on cellulose stationary phases with HPLC— DAD reversed-phase chromatography was performed to assess a cheap and rapid technique in the identification process of major phenolic constituents. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging tests, expressed as IC50, revealed the most beneficial results for the fraction after alkaline hydrolysis and yielded 205 ± 8 μg mL−1.

Słowa kluczowe

EN

phenolics; 2,2-diphenyl-1-picrylhydrazyl; radical-scavenging activity; HPLC; chemical composition; warted spindle

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2015
• Tom: Vol. 27, no. 4
• Strony: 743--754
• Opis fizyczny: Bibliogr. 21 poz., rys., tab.

Twórcy

autor

Kukula-Koch W.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki St., 20-093 Lublin, Poland

autor

Widelski J.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki St., 20-093 Lublin, Poland

autor

Koch W.

• Chair and Department of Food and Nutrition, Medical University of Lublin, 4a Chodzki St., 20-093 Lublin, Poland

autor

Głowniak K.

• Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki St., 20-093 Lublin, Poland

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