Alterations in fatty acid composition of Cunninghamella echinulata lipids induced by orange essential oil

• Autorzy: Moustogianni A. , Bellou S. , Triantaphyllidou I. E. , Aggelis G.

Identyfikatory

DOI

10.14799/ebms237

• Języki publikacji: EN

Abstrakty

EN

The growth of Cunninghamella echinulata on raw glycerol as sole carbon source was positively affected by the presence of essential oil (EO) of orange in the growth medium. However, significant alterations in the fatty acid composition were observed since the biosynthesis of PUFA was inhibited, especially in the late fermentation steps. Refereeing to the ratios ^∆9C18:1/C18:0, ^∆9,12C18:2/^∆9C18:1 and ^∆6,9,12C18:3/^∆9,12C18:2 used as indices for ∆9, ∆12 and ∆6 desaturase activity, respectively, we suggest that in the presence of EO, the activity of ∆9 desaturase involved in the desaturation of stearic acid was significantly increased. During this process both ∆12 and ∆6 desaturases involved in the desaturation of oleic and linoleic acids, respectively, decreased. We conclude that EO could be used for the in vivo modification of microbial lipids.

Słowa kluczowe

EN

Cunninghamella echinulata; essential oil; fatty acid desaturases; glycerol

PL

Cunninghamella echinulata; desaturaza kwasów tłuszczowych; olejek eteryczny; glicerol

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2014
• Tom: Vol. 10, No. 1
• Strony: 1--7
• Opis fizyczny: Bibliogr. 41 poz., tab.

Twórcy

autor

Moustogianni A.

• Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece

autor

Bellou S.

• Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece

autor

Triantaphyllidou I. E.

• Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece

autor

Aggelis G.

• Unit of Microbiology, Division of Genetics, Cell and Development Biology, Department of Biology, University of Patras, Patras, Greece; Phone: [phone]
• Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

Bibliografia

• AFNOR. 1984. Recueil des normes francaises des corps gras, grains oleagineux et produits dérives, third ed. Association Francaise pour Normalisation. 95 p. Paris.
• Aggelis, G., M. Komaitis. 1999. Enhancement of single cell oil production by Yarrowia lipolytica growing in the presence of Teucrium polium L. aqueous extract. Biotechnology Letters 21: 747-749.
• Bagamboula, C.F., M. Uyttendaele, J. Debevere. 2004. Inhibitory effect of thyme and basil essential oils, carvacrol, thymol, estragol, linalool and p-cymene towards Shigella sonnei and S. flexneri. Food Microbiology 21: 33-42.
• Bakkali, F., S. Averbeck, D. Averbeck, M. Idaomar. 2008. Biological effects of essential oils - a review. Food and Chemical Toxicology 46: 446-475.
• Bellou, S., A. Moustogianni, A. Makri, G. Aggelis. 2012. Lipids containing polyunsaturated fatty acids synthesized by zygomycetes grown on glycerol. Applied Biochemistry and Biotechnology 166: 146-158.
• Bellou, S., A. Makri, D. Sarris, K. Michos, P. Rentoumi, A. Celik, S. Papanikolaou, G. Aggelis. 2014. The olive mill wastewater as substrate for single cell oil production by Zygomycetes. Journal of Biotechnology 170: 50-59.
• Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. International Journal of Food Microbiology 94: 223-253.
• Cerrutti, P., S.M. Alzamora. 1996. Inhibitory effects of vanillin on some food spoilage yeasts in laboratory media and fruit purees. International Journal of Food Microbiology 29: 379-386.
• Certík, M., Z. Adamechová, K. Laoteng. 2012. Microbial production of γ-linolenic acid: Submerged versus solid-state fermentations. Food Science and Biotechnology 21: 921-926.
• Chatzifragkou, A., A. Makri, A. Belka, S. Bellou, M. Mavrou, M. Mastoridou, P. Mystrioti, G. Onjaro, G. Aggelis, S. Papanikolaou. 2011. Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy 36: 1097-1108.
• Dimou, D.M., A. Georgala, M. Komaitis, G. Aggelis. 2002. Mycelial fatty acid composition of Pleurotus spp. and its application in the intrageneric differentiation. Mycological Research 106: 925-929.
• Easterling, E.R., W.T. French, R. Hernandez, M. Licha. 2009. The effect of glycerol as a sole and secondary substrate on the growth and fatty acid composition of Rhodotorula glutinis. Bioresource Technology 100: 356-361.
• Ethier, S., K. Woisard, D. Vaughan, Z. Wen. 2011. Continuous culture of the microalgae Schizochytrium limacinum on biodieselderived crude glycerol for producing docosahexaenoic acid. Bioresource Technology 102: 88-93.
• Fakas, S., S. Papanikolaou, M. Galiotou-Panayotou, M. Komaitis, G. Aggelis. 2006. Lipids of Cunninghamella echinulata with emphasis to γ-linolenic acid distribution among lipid classes. Applied Microbiology and Biotechnology 73: 676-683.
• Fakas, S., M. Galiotou-Panayotou, S. Papanikolaou, M. Komaitis, G. Aggelis. 2007. Compositional shifts in lipid fractions during lipid turnover in Cunninghamella echinulata. Enzyme and Microbial Technology 40: 1321-1327.
• Fakas, S., S. Papanikolaou, A. Batsos, M. Galiotou-Panayotou, A. Mallouchos, G. Aggelis. 2009. Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina. Biomass Bioenergy 33: 573–580.
• Fan, X., R. Burton, Y. Zhou. 2010. Glycerol (byproduct of biodiesel production) as a source for fuels and chemicals - mini review. The Open Fuels and Energy Science Journal 3: 17-22.
• Folch, J., M. Lees, G. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 199: 833-841.
• Gema, H., A. Kavadia, D. Dimou, V. Tsagou, M. Komaitis, G. Aggelis. 2002. Production of gamma-linolenic acid by Cunninghamella echinulata cultivated on glucose and orange peel. Applied Microbiology and Biotechnology 58: 303-307.
• Ghfir, B., J.L. Fonvieille, Y. Koulali, R. Ecalle, R. Dargent. 1994. Effect of essential oil of Hyssopus officinalis on the lipid composition of Aspergillus fumigatus. Mycopathologia 126: 163-167.
• Ghfir, B., J.L. Fonvieille, R. Dargent. 1997. Influence of essential oil of Hyssopus officinalis on the chemical composition of the walls of Aspergillus fumigatus (Fresenius). Mycopathologia 138: 7-12.
• Hammer, K.A., C.F. Carson, T.V. Riley. 1999. Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology 86: 985-990.
• Helal, G.A., M.M. Sarhan, A.N.K. Abu Shahla, E.K. Abou El-Khair. 2006a. Effects of Cymbopogon citratus L. essential oil on the growth and morphogenesis of Saccharomyces cerevisiae ML2-strain. Journal of Basic Microbiology 46: 375-386.
• Helal, G.A., M.M. Sarhan, A.N.K. Abu Shahla, E.K. Abou El-Khair. 2006b. Effects of Cymbopogon citratus L. essential oil on the growth, lipid content and morphogenesis of Aspergillus niger ML2-strain. Journal of Basic Microbiology 46: 456-469.
• Helal, G.A., M.M. Sarhan, A.N.K. Abu Shahla, E.K. Abou El-Khair. 2007. Effects of Cymbopogon citratus L. essential oil on the growth, morphogenesis and aflatoxin production of Aspergillus flavus ML2-strain. Journal of Basic Microbiology 47: 5-15.
• Karanika, M.S., M. Komaitis, G. Aggelis. 2001. Effect of aqueous extracts of some plants of Lamiaceae family on the growth of Yarrowia lipolytica. International Journal of Food Microbiology 64: 175-181.
• Klempova, T., E. Basil, A. Kubatova, M. Certik. 2013. Biosynthesis of gamma-linolenic acid and beta-carotene by zygomycetes fungi. Biotechnology Journal 8: 794-800.
• Lucini, E.I., M.P. Zunino, M.L. López, J.A. Zygadlo. 2006. Effect of monoterpenes on lipid composition and sclerotial development of Sclerotium cepivorum Berk. Journal of Phytopathology 154: 441-446.
• Meesters, P.A.E.P., G.N.M. Huijberts, G. Eggink. 1996. High-celldensity cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. Applied Microbiology and Biotechnology 45: 575-579.
• Mishra, A.K., N.K. Dubey. 1994. Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities. Applied and Environmental Microbiology 60: 1101-1105.
• Moreton, R.S. 1985. Modification of fatty acid composition of lipid accumulating yeasts with cyclopropene fatty acid desaturase inhibitors. Applied Microbiology and Biotechnology 22: 42-45.
• Papanikolaou, S., L. Muniglia, I. Chevalot, G. Aggelis, I. Marc. 2003. Accumulation of a cocoa-butter-like lipid by Yarrowia lipolytica cultivated on agro-industrial residues. Current Microbiology 46: 124-130.
• Papanikolaou, S., S. Sarantou, M. Komaitis, G. Aggelis. 2004. Repression of reserve lipid turnover in Cunninghamella echinulata and Mortierella isabellina cultivated in multiple-limited media. Journal of Applied Microbiology 97: 867-874.
• Papanikolaou, S., O. Gortzi, E. Margeli, I. Chinou, M. Galiotou-Panayotou, S. Lalas. 2008. Effect of Citrus essential oil addition upon growth and cellular lipids of Yarrowia lipolytica yeast. European Journal of Lipid Science and Technology 110: 997-1006.
• Pinto, E., L. Vale-Silva, C. Cavaleiro, L. Salgueiro. 2009. Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. Journal of Medical Microbiology 58: 1454-1462.
• Sánchez-González, L., M. Cháfer, A. Chiralt, C. González-Martínez. 2010. Physical properties of edible chitosan films containing bergamot essential oil and their inhibitory action on Penicillium italicum. Carbohydrate Polymers 82: 277-283.
• Shimizu, S., K. Akimoto, Y. Shinmen, H. Kawashima, M. Sugaro, H. Yamada. 1991. Sesamin is a potent and specific inhibitor of ∆5 desaturase in polyunsaturated fatty acid biosynthesis. Lipids 26: 512-516.
• Silva, J., W. Abebe, S.M. Sousa, V.G. Duarte, M.I.L. Machado, F.J.A. Matos. 2003. Analgesic and anti-inflammatory effects of essential oils of eucalyptus. Journal of Ethnopharmacology 89: 277-283.
• Tranter, H.S., C. Tassou, G.J. Nychas. 1993.The effect of the olive phenolic compound, oleuropein, on growth and enterotixin B production by Staphylococcus aureus. Journal of Applied Bacteriology 74: 253–259.
• Zhu, L.Y., M.H. Zong, H. Wu. 2008. Efficient lipid production with Trichosporon fermentans and its use for biodiesel preparation. Bioresource Technology 99: 7881-7885.
• Zikou, E., A. Chatzifragkou, A.A. Koutinas, S. Papanikolaou. 2013. Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans. Journal of Applied Microbiology 114: 1020-1032.