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Biodegradation of whey waste in a continuous stirred-tank bioreactor

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Post-production whey has a high concentration of proteins and lactose that has to be decreased 500-fold before disposal in the environment. The paper describes goat whey biodegradation in a continuous stirred tank reactor (CSTR) using a Bacillus licheniformis strain. The data obtained emphasize the effect of excess organic compounds on growth rate. Accordingly, the Luong equation was applied to describe the batch process while accounting for substrate inhibition; the constants vmax = 2.29·10–4 1/s, Km = 4.79 g/dm3, Ki = 6.03 g/dm3, and n = 1.1 were experimentally obtained. According to accepted theory, the continuous process can be expressed using Monod’s equation (only for the rising side of the kinetic graph) in a restricted range of substrate concentrations (for glucose, up to 2.16 g/dm3). The estimated values of the constants were vmax = 8.52·10–5 1/s and Km = 0.45 g/dm3. The selected strain effectively decreased the lactose content in whey. At residence times above 30 h, almost total decrease in lactose content was observed that met the requirements of the Polish Ministry of Environmental Protection. The biodegradation of proteins was much slower, but their content does not greatly influence the required BOD and COD values.
Rocznik
Strony
97--107
Opis fizyczny
Bibliogr. 32 poz., rys.
Twórcy
autor
  • Division of Bioprocess and Biomedical Engineering, Wrocław University of Technology, ul. Norwida 4/6, 50-373, Wrocław, Poland
  • Division of Bioprocess and Biomedical Engineering, Wrocław University of Technology, ul. Norwida 4/6, 50-373, Wrocław, Poland
Bibliografia
  • [1] GONZALEZ SISO M.I., The biotechnological utilization of cheese whey, Bioresour. Technol., 1996, 57, 1.
  • [2] MAWSON A.J., Bioconversation for whey utilization and waste abatement, Bioresour. Technol., 1994, 47, 195.
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  • [4] BERNSTAIN D., EVERSON T., Protein production from acid whey via fermentation, in food processing waste management, Cornell Agricultural Waste Management Conference, Cornell University, New York, 1973, 103.
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  • [6] ALMECIJA M.C., IBANEZ R., GUADIX A., GUADIX E.M., Effect of pH on the fractionation of whey proteins with a ceramic ultrafiltration membrane, J. Membr. Sci., 2007, 288, 28.
  • [7] METASMUURONEN S., NYSTROM M., Enrichment of -lactolabumin from diluted whey with polymeric ultrafiltration membranes, J. Membr. Sci., 2009, 337, 248.
  • [8] SANMARTIN B., DIAZ O., RODRIGUEZ-TURIENZO L., COBOS A., Composition of caprine whey protein concentrates produced by membrane technology after clarification of cheese whey, Small Rum. Res., 2012, 105, 186.
  • [9] DLUGOSZ E., Wastewater treatment with activated sludge, Retrieved from http://www.e-biotechnologia. pl/Artykuly/Oczyszczanie-sciekow-metoda-osadu-czynnego/, 30.01.2013 (in Polish).
  • [10] SARKER.B., CHAKRABARTI P.P., VIJAYKUMAR A., KALE V., Wastewater treatment in dairy industries – possibility of refuse, Desalination, 2006, 195, 141.
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  • [12] SAKAI K., YAMANAMI T., Thermotolerant Bacillus licheniformis TY7 produces optically active l-lactic acid from kitchen refuse under open condition, J. Biosci. Bioeng., 2006, 102 (2), 132.
  • [13] PENESAR P.S., KENNEDY J.F., GANDHII D.N., BUNKO K., Bioutilisation of whey for lactic acid production, Food Chem., 2007, 105, 1.
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  • [20] HYNES W.M., Handbook of chemistry and physics, CRC Press Taylor and Francis Group, Boca Raton 2011.
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  • [24] ZHANG X.-W., GONG X.D., CHEN F., Dynamics and stability analysis of the growth and astaxanthin production system of Haematococcus pluvialis, J. Industr. Microbiol. Biotechnol., 1999, 23 (2), 133.
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  • [27] REKTOR A., VATAI G., Membrane filtration of mozzarella whey, Desalination, 2004, 162, 279.
  • [28] LE T.T., CABALTICA A.D., BUI V.M., Membrane separations in dairy processing, J. Food Res. Technol., 2014, 2 (1), 1.
  • [29] JAUREGI P., WELDERUFAEL F.T., Added-value protein products from whey. Extraction, fractionation, separation, purification, Natura Foods, 2010, 9 (4), 13.
  • [30] TRUSEK-HOLOWNIA A., NOWORYTA A., Biological regeneration of liquid sorbents after industrial purification of outlet gases, Chem. Process Eng., 2012, 33 (4), 667.
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Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-4d534a14-4afb-4e23-acc8-be5f5caa7683
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