Separation of matrine and oxymatrine from Sophora flavescens extract through cation exchange resin coupled with macroporous absorption resin

• Autorzy: Chen H. , Luo S. , Zheng X. , Fan H.

Identyfikatory

DOI

10.1515/pjct-2016-0026

• Języki publikacji: EN

Abstrakty

EN

A simple method for separation of matrine and oxymatrine from Sophora flavescens was developed with cation exchange resin coupled with macroporous resin. Based on the adsorption characteristics of matrine and oxymatrine, 001×732 cation exchange resin was used to absorb target alkaloids for removing most of the foreign matter, while BS-65 macroporous resin was chosen to purify these alkaloids. The result showed that the equilibrium adsorption data of matrine and oxymatrine on 001×732 resin and BS-65 resin at 30°C was fitted to Langmuir isotherm and Freundlich isotherm, respectively. The contents of matrine and oxymatrine were increased from 0.73% and 2.2% in the crude extract of the root of Sophora flavescens to 67.2% and 66.8% in the final eluent products with the recoveries of 90.3% and 86.9%, respectively.

Słowa kluczowe

EN

Sophora flavescens; BS-65 macroporous resin; 001×732 cation exchange resin; separation; alkaloids

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2016
• Tom: Vol. 18, nr 2
• Strony: 31--39
• Opis fizyczny: Bibliogr. 26 poz., rys., tab.

Twórcy

autor

Chen H.

• First Affiliated Hospital of Shantou University Medical College, Department of Pharmacy, Shantou 515041, China

autor

Luo S.

• Guangdong Pharmaceutical University, College of Pharmacy, Guangzhou 510006, China

autor

Zheng X.

• Guangdong Pharmaceutical University, College of Pharmacy, Guangzhou 510006, China

autor

Fan H.

• Guangdong Pharmaceutical University, College of Pharmacy, Guangzhou 510006, China

Bibliografia

• 1. Pharmacopoeia Commission of People’s Republic of China. (2010). Pharmacopoeia of People’s Republic of China (188). Beijing: The Chemical Industry Press.
• 2. Song, G.B., Luo, Q., Qin, J., Wang, L., Shi, Y.S. & Sun, C.X. (2006). Effects of oxymatrine on proliferation and apoptosis in human hepatoma cells. Coll. Surf. B. 48, 1–5. DOI: 10.1177/1533034615587616.
• 3. Ueng, Y.F., Chen, C.C., Tsai, C.C. & Souček, P. (2009). Different inductive profiles of hepatic cytochrome P450s by the extracts of Sophora flavescens in male and female C57BL/6JNarlmice. J. Ethnopharmac. 126, 437–446. DOI: 10. 1016/j.jep.2009.09.018.
• 4. Wan, X.Y., Luo, M., Li, X.D. & He, P. (2009). Hepatoprotective and anti-hepatocarcinogenic effects of glycyrrhizin and matrine. Chem. Biol. Interact. 181, 15–19. DOI: 10.1016/j. cbi.2009.04.013.
• 5. Luo, C., Zhu, Y.L., Jiang, T.J., Lu, X.Y., Zhang, W., Jing, Q.F., Li, J., Pang, L.R., Chen, K.J., Qiu, F.M., Yu, X.Y., Yang, J.H. & Huang, J. (2007). Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic moleculesof Bcl-2 family. Toxicol. Lett. 229, 245–252. DOI: 10.1016/j.tox.2006.10. 020.
• 6. Suo, Z.W., Liu, Y., Ferreri, M., Zhang, T., Liu, Z.J., Mu, X. & Han, B. (2009) Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells. J. Ethnopharmacl. 125, 404–409. DOI: 10.1016/j.jep.2009.07.023.
• 7. Gao, H.Y., Li, G.Y., Lou, M.M., Li, X.Y., Wei, X.Y. & Wang, J.H. (2012). Hepatoprotective effect of Matrine salvianolic acid B salt on Carbon Tetrachloride – Induced Hepatic Fibrosis. J. Inflamm. 9, 16. DOI: 10.1186/1476-9255-9-16.
• 8. Zhang, H.L., He, C.L., Yang, Q.E., Yang, X.J. & Han, C.X. (2006). Study on separation and toxicity to Mus musculus of oxymatrine. Nat. Prod. Res. Dev. 18, 279–281. DOI: 1001-6880(2006)02-0279-04.
• 9. Hu, Y.L., Guo, L. & Jia, X.H. (2007). Study on separation and purification technology of Sophara alopecuroides L. Food Sci. 28, 32–36. DOI: 1002-6630 (2007)01-0032-05.
• 10. An, G. (2006). Studies on technology for extracting and purifying matrine and oxymatrine from Sophora flavescens. J. Southwest For. Coll. 26, 37–39. DOI: 1003-7179(2006)02-0037-03.
• 11. Yang, L.M., Zhou, L., Ran, X.Y., Zhang, W.G. & Wu, L. (2006). Separation of oxymatrine from Sophora flavescens by macroporous absorption resin. J. North West Sci. Tech. Univ. Agri. For. 34, 129–132. DOI: 10.3321/j.issn:1671-9387. 2006.11.028.
• 12. Pedruzzi, I., Borges da Silva, E.A. & Rodrigues, A.E. (2008). Selection of resins, equilibrium and sorption kinetics of lactobionic acid, fructose, lactose and sorbitol. Sep. Purif. Technol. 63, 600–611. DOI: 10.1016/j.seppur.2014.08.020.
• 13. Fu, Y.J., Zu, Y.G., Liu,W., Hou, C.L. & Chen, L.Y. (2007). Preparative separation of vitexin and isovitexin from Pigeonpea extracts with macroporous resins. J. Chromatogr. A. 1139, 206–213. DOI: 10.1016/j.chroma.2006.11.015.
• 14. Fu, B.Q., Liu, J., Li, H., Li, H., Lee, Frank S.C. & Wang, X.R. (2005). The application of macroporous resins in the separation of licorice flavonoids and glycyrrhizic acid. J. Chromatogr. A 1089, 18–24. DOI: 10.1016/j.chroma.2005.06.051
• 15. Tang, Z.G., Zhou, R.Q. & Duan, Z.T. (2001) Adsorption and desorption behaviour of taurine on macroporous adsorption resins. J. Chem. Technol. Biotechnol. 76, 752–756. DOI: 10.1002/jctb.446.
• 16. Wan, J.B., Zhang, Q.W., Ye, W.C. & Wang, Y.T. (2008). Quantifi cation and separation of protopanaxatriol and protopanaxadilo type saponins from Panax notoginseng with macroporous resins. Sep. Purif. Technol. 60, 198–205. DOI: 10.1016/j. seppur.2014.08.020.
• 17. Cren, Érika C., Cardozo Filho, L., Silva, Edson A. & Meirelles, Antonio J.A. (2009). Breakthrough curves for oleic acid removal from ethanolic solutions using a strong anion exchange resin. Sep. Purif. Technol. 69, 1–6. DOI: 10.1016/j.seppur.2014.08.020.
• 18. Silva, E.M., Pompeu, D.R., Larondelle, Y. & Rogez, H. (2007). Optimisation of the adsorption of polyphenols from Inga edulis leaves on macroporous resins using an experimental design methodology. Sep. Purif. Technol. 53, 274–280. DOI: 10.1016/j.seppur.2014.08.020.
• 19. Zhang, B., Yang, R.Y., Zhao, Y. & Liu, C.Z. (2008). Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins. J. Chromatogr. B. 867, 253–258. DOI: 10.1016/j.jchromb.2008.04.016
• 20. Bernardin, F.E. (1985). In: F.L. Slejko (Eds.), Experimental design and testing of adsorption and adsorbates (1–35). Marcel Dekker, New York: Adsorption Technology.
• 21. Wang, F., Wang, L.J., Li, J.S., Sun, X.Y. & Han, W.Q. (2009). Adsorption behavior and mechanism of cadmium on strong-acid cation exchange resin. Trans. Nonferrous Met. Soc. China 19, 740–744. DOI: 10.1016/S1003-6326(08)60343-X.
• 22. Juang, R.S. & Shiau, J.Y. (1999). Adsorption isotherms of phenols from water onto macroreticular resins. J. Hazard. Mater. B. 70, 171–183. DOI: 10.1016/S0304-3894(99)00152-1.
• 23. Baskaralingam, P., Pulikesi, M., Elango, D., Ramamurthi, V. & Sivanesan, S. (2006). Adsorption of acid dye onto organobentonite. J. Hazard. Mater. B. 128, 138–144. DOI: 10.1016/j.jhazmat.2005.07.049.
• 24. Langmuir, I. (1918). The adsorption of gases on plane surfaces of glass, mica and platinum. J. Am. Chem. Soc. 40, 1361–1402. DOI: 10.1021/Ja02242a004.
• 25. Freundlich, H. (1909). The theory of adsorption. Z. Chem. Ind. Kolloide 3, 212–220. DOI: 10.1007/Bf01427691.
• 26. Treybal, R.E. (1981). Mass-Transfer Operations (3rd ed.). Singapore: McGraw-Hill International Companies.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.