Three phase partitioning as a rapid and efficient method for purification of plant-esterase from wheat flour

• Autorzy: Dong Liang , He Linxin , Huo Danqun

Identyfikatory

DOI

10.2478/pjct-2020-0015

• Języki publikacji: EN

Abstrakty

EN

Three-phase partitioning (TPP) was used to purify plant-esterase from wheat flour. Effect of various process parameters has been evaluated and plant-esterase was purified to 11.35-fold by optimized single step TPP system (50%, (w/v) (NH₄)₂ SO₄ saturation, 1:1 (v/v) ratio of crude extract: t-butanol at pH 4).The enzyme was found to be exclusively partitioned in the aqueous phase. Using TPP system, plant-esterase quickly purified to homogeneity with very high purity and activity. On the basis of single factor research, purification process was optimized by using response surface method, established a new type of high effcient purification plant-esterase method. To the best of our knowledge, this is the first report for purification and characterization of plant-esterase by using three phase partitioning (TPP). The results indicated that, TPP is a simple, quick, economical and very attractive process for purification of plant-esterase compared to conventional chromatographic protocols.

Słowa kluczowe

EN

Purification; Plant esterase; Three phase partitioning

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2020
• Tom: Vol. 22, nr 2
• Strony: 42--49
• Opis fizyczny: Bibliogr. 39 poz., rys., tab.

Twórcy

autor

Dong Liang

• Sichuan University of Science & Engineering, Zigong 643000, China

autor

He Linxin

• Sichuan University of Science & Engineering, Zigong 643000, China

autor

Huo Danqun

• Key Laboratory of Biorheology Science and Technology, Ministry of Education, College of Bioengineering, Chongqing University, Chongqing 400044, China

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).