Rapd-Analysis of Flax Varieties of the Ukrainian National Collection

• Autorzy: Kovalenko Ihor , Vereshchahin Ihor , Butenko Yevheniia , Kandyba Natalia , Onychko Viktor , Bakumenko Olha , Kovalenko Vladyslav , Klochkova Tetiana

Identyfikatory

DOI

10.12912/27197050/146384

• Języki publikacji: EN

Abstrakty

EN

The article presents the results of research on the use of RAPD (Random Amplified Polymorphic DNA) molecular primers, linked to the sign of fiber content in the stems of flax. The main purpose of the research is to identify genetic polymorphism based on the fiber content, inter- and intraspecific genetic relationships between domestic and foreign flax varieties and hybrids. Realization of this aim will allow the number of tasks: 1) to increase the possibilities for successfully identify flax varieties and hybrids, 2) group the breeding material according to its genetic affinity, which will optimize the selection of pairs for crossing, 3) it is better to integrate traditional breeding methods (hybridization, selection, mutagenesis) with molecular biology methods for creation flax varieties with specified parameters of valuable economic traits and, as a consequence, to make selection most successful. 24 varieties and hybrids of flax were investigated using the method of polymerase chain reaction and separation of DNA fragments by gel electrophoresis. A small number of loci on electrophoregrams were detected, which indicates a small affinity of the selection material, which was also confirmed by the use of cluster analysis.

Słowa kluczowe

EN

Linum usitatissimum L.; variety sample; RAPD analysis; Random Amplified Polymorphic DNA; primer; locus; cluster

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2022
• Tom: Vol. 23, iss. 3
• Strony: 1--6
• Opis fizyczny: Bibliogr. 30 poz., rys.

Twórcy

autor

Kovalenko Ihor

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0003-4957-2352

autor

Vereshchahin Ihor

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0002-6589-5138

autor

Butenko Yevheniia

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0001-8904-519X

autor

Kandyba Natalia

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0001-6548-3670

autor

Onychko Viktor

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0003-0584-319X

autor

Bakumenko Olha

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0003-1625-7401

autor

Kovalenko Vladyslav

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0002-9830-3370

autor

Klochkova Tetiana

• Sumy National Agrarian University, H. Kondratieva St., 160, Sumy, 40021, Ukraine

• https://orcid.org/0000-0002-1173-6211

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