Rat cancer cells necrosis induced by ultrasound

• Autorzy: Secomski W. , Bilmin K. , Kujawska T. , Nowicki A. , Grieb P.
• Języki publikacji: EN

Abstrakty

EN

Sonodynamic therapy is the ultrasound dependent enhancement of the cytotoxic activities of certain drugs called sonosensitizers. The study of therapeutic efficacy of ultrasound is always preceded by in-vitro tests. In this work, two in-vitro sonication procedures were compared. One with the transducer positioned bellow the cell colony, radiating upward, with standing wave reflected from the water-air surface, the second, in the free field conditions. Efficiency of the cancer cells necrosis caused by ultrasound was compared with acoustical field intensity ISPTA measured by a hydrophone. The standing wave conditions effectively increased the intensity of the ultrasonic wave at the level of cells. To achieve 50% of cell viability, the intensity ISATA, decreased from 5.8 W/cm2 to 0.3 W/cm2. In summary, sonication in the standing wave conditions can effectively and reproducibly destroy cells by ensuring the sterility and without the risk of overheating.

• Wydawca: Polskie Towarzystwo Akustyczne. Oddział Gdański
• Czasopismo: Hydroacoustics
• Rocznik: 2014
• Tom: Vol. 17
• Strony: 179--186
• Opis fizyczny: Bibliogr. 19 poz., rys.

Twórcy

autor

Secomski W.

• Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5b, 02-106 Warszawa, Poland

autor

Bilmin K.

• Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warszawa, Poland

autor

Kujawska T.

• Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5b, 02-106 Warszawa, Poland

autor

Nowicki A.

• Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego 5b, 02-106 Warszawa, Poland

autor

Grieb P.

• Mossakowski Medical Research Centre, Polish Academy of Sciences, Pawińskiego 5, 02-106 Warszawa, Poland

Bibliografia

• [1] A. Castanoa, T. Demidovaa, M. Hamblin, Mechanisms in photodynamic therapy: part one-photosensitizers, photochemistry and cellular localization, Photodiagnosis and Photodynamic Therapy Vol. 1, 279-293, 2004.
• [2] A. Castanoa, T. Demidovaa, M. Hamblin, Mechanisms in photodynamic therapy: part two-cellular signaling, cell metabolism and modes of cell death, Photodiagnosis and Photodynamic Therapy Vol. 2, 1-23, 2005.
• [3] S. Umemura, K. Kawabata, K. Sasaki, N. Yumita, K. Umemura, R. Nishigaki, Recent advances in sonodynamic approach to cancer therapy, Ultrasonics Sonochemistry Vol. 3, S187-S191, 1996.
• [4] K. Tachibana, L. B. Feril, Y. Ikeda-Dantsuji, Sonodynamic therapy, Ultrasonics Vol. 48, 253–259, 2008.
• [5] I. Rosenthal, J. Sostaric, P. Riesz, Sonodynamic therapy-a review of the synergistic effects of drugs and ultrasound, Ultrasonics Sonochemistry Vol. 11, 349–363, 2004.
• [6] L. B. Feril, K. Tachibana, K. Ogawa, K. Yamaguchi, I. Solano, Y. Irie, Therapeutic potential of low-intensity ultrasound (part 1): thermal and sonomechanical effects, J Med Ultrasonics Vol. 35, 153–160, 2008.
• [7] L. B. Feril, K. Tachibana Y. Ikeda-Dantsuji, H. Endo, Y. Harada, T. Kondo, R. Ogawa, Therapeutic potential of low-intensity ultrasound (part 2): biomolecular effects, sonotransfection, and sonopermeabilization, J Med Ultrasonics Vol. 35, 161–167, 2008.
• [8] N. Yumita, K. Kawabata, K. Sasaki, S. Umemura, Sonodynamic effect of erythrosin B on sarcoma 180 cells in vitro, Ultrasonics Sonochemistry Vol. 9, 259–265, 2002.
• [9] S. Yamaguchi, H. Kobayashi, T. Narita, K. Kanehira, S. Sonezaki, N. Kudo, Y. Kubota, S. Terasaka, K. Houkin, Sonodynamic therapy using water-dispersed TiO2- polyethylene glycol compound on glioma cells: Comparison of cytotoxic mechanism with photodynamic therapy, Ultrasonics Sonochemistry Vol. 18, 1197–1204, 2011.
• [10] H. Tsuru, H. Shibaguchi, M. Kuroki, Y. Yamashita, M. Kuroki, Tumor growth inhibition by sonodynamic therapy using a novel sonosensitizer, Free Radical Biology and Medicine Vol. 53, 464–472, 2012.
• [11] J. E. Maalouf, J.-C. Béra, L. Alberti, D. Cathignol, J.-L. Mestas, In vitro sonodynamic cytotoxicity in regulated cavitation conditions, Ultrasonics Vol. 49, 238–243, 2009.
• [12] S. Iwabuchi, M. Ito, J. Hata, T. Chikanishi, Y. Azuma, H. Haro, In vitro evaluation of low-intensity pulsed ultrasound in herniated disc resorption, Biomaterials Vol. 26, 7104-7114, 2005.
• [13] T. Kujawska, W. Secomski, K. Bilmin, A. Nowicki, P. Grieb, Impact of thermal effects induced by ultrasound on viability of rat C6 glioma cells, Ultrasonics Vol. 54, 1366- 1372, 2014.
• [14] L. B. Feril, T. Kondo, Q.-L. Zhao, R. Ogawa, Enhancement of hyperthermia-induced apoptosis by non-thermal effects of ultrasound, Cancer Letters Vol. 178, 63–70, 2002.
• [15] K.Milowska, T.Gabryelak, Enhancement of ultrasonically induced cell damage by phthalocyanines in vitro, Ultrasonics Vol. 48, 724–730, 2008.
• [16] J.R. Eisenbrey, P. Huang, J. Hsu, M.A. Wheatley, Ultrasound triggered cell death in vitro with doxorubicin loaded poly lactic-acid contrast agents, Ultrasonics Vol. 49, 628-633, 2009.
• [17] A. Watanabe, S. Kakutani, R. Ogawa, S. Lee, T. Yoshida, A. Morii, G. Kagiya, L. B. Feril, Jr. H. Fuse, T. Kondo, Construction of artificial promoters sensitively responsive to sonication in vitro, J Med Ultrasonics Vol. 36, 9–17, 2009.
• [18] Encyclopedia of acoustics, edited by M. J. Crocker, John Wiley & Sons, New York, 12, 1997.
• [19] Ultrasonic Exposimetry, edited by M.C. Ziskin, P. A. Lewin, CRC Press, Boca Raton, 91-125, 1993.