Biosensory proteinowe i ich właściwości fluorescencyjne

• Autorzy: Kłos-Witkowska A.

Warianty tytułu

EN

Protein based biosensors and their fluorescent properties

• Języki publikacji: PL

Abstrakty

PL

W pracy zostały opisane biosensory proteinowe oraz ich właściwości fluorescencyjne. Przybliżono podstawy projektowania biosensorów proteinowych oraz strategii stosowanych przy projektowaniu sensorów białkowych:rational protein design (racjonalne projektowanie), directed evolution (ewolucja ukierunkowana), de novo design (projektowanie białek de novo).Zestawiono anality oraz proteiny receptorowe wykorzystywane w badaniach fluorescencyjnych. Opisano zastosowania biosensorów w medycynie.

EN

This paper describes the basics of protein biosensors with particular emphasis on their fluorescent properties. Biosensors are classified on receptor layers with particular emphasis on protein biosensors. There is brought closer the base of protein sensor design, taking into account three strategies commonly use in protein engineering in order to improve sensivity, selectivity and stability of protein biosensor: rational design, directed evolution, de novo design. The main construction problems encountered in the design of biosensor and ways of they solving have been described. The recent research on fluorescent protein biosensors receptor layer is presented. In Table 1 there is given the list of proteins and appropriate fluorescent methods: FRET (Föster Resonance energy Transfer), change in fluorescence emission spectra, for Zn2+, Glc ,Gal ,Fruc, Arab, DMAPIP-b, Ca2+, H2O2, Ca2+, β-CD,CB6, CB7, CFP detection. There is described the use of fluorescent protein based biosensors for clinical application. Biosensors as modern and widely used tools in environmental, medical, food industry have been presented and the future trends in scientific research have been given in the paper.

Słowa kluczowe

PL

biosensor; fluorescencja; proteina; analit

EN

biosensor; fluorescence; protein

• Wydawca: Wydawnictwo PAK
• Czasopismo: Pomiary Automatyka Kontrola
• Rocznik: 2014
• Tom: R. 60, nr 6
• Strony: 378--381
• Opis fizyczny: Bibliogr. 40 poz., tab.

Twórcy

autor

Kłos-Witkowska A.

• Akademia Techniczno-Humanistyczna w Bielsku Białej, Wydział Budowy Maszyn i Informatyki, Katedra Elektrotechniki i Automatyki, 43-309 Bielsko-Biała, ul. Willowa 2

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