Quantitative analysis of berberine in Berberis aristata fruits and in a traditional anti-inflammatory unani formulation by use of a validated HPLC method

• Autorzy: Kamal Y. T , Singh M. , Tamboli E. T , Parveen R , Ahmad S.
• Języki publikacji: EN

Abstrakty

EN

A validated reversed-phase HPLC method has been developed for quantitative analysis of berberine in Berberis aristata fruits and in a polyherbal formulation. Separation of berberine was achieved on a C18 column with a mobile phase consisting of a 10–80% acetonitrile gradient in 0.05% aqueous orthophosphoric acid. The flow rate was 1 mL min^-1. Detection was at 266 nm. A sharp, well defined peak was obtained at a retention time of 10.0 ± 0.4 min. The method was validated in accordance with ICH guidelines for accuracy, precision, robustness, and the limits of detection (LOD) and quantification (LOQ). Results from linear regression analysis were indicative of a good linear relationship (r2 = 0.998 ± 0.0011) in a wide concentration range (5–500 μ g mL^-1). LOD and LOQ were 1.5 and 5.3 μg mL^-1, respectively. Satisfactory recovery results (94.6–103.1%) were obtained by the method of standard addition. Intra-day, inter-day, and intersystem precision was satisfactory, with relative standard deviation in the range 0.7–1.8%. The berberine content of fruit of Berberis aristata and the herbal formulation were 0.033% and 0.0089% (w/w), respectively. This HPLC method for quantification of berberine can be used for quality control and standardization of several crude drugs and different herbal formulations in which berberine is present as a phyto constituent.

Słowa kluczowe

EN

berberine; HPLC; Berberis aristata; Unani formulation

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 1
• Strony: 157--168
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Kamal Y. T

• Hamdard University Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy Hamdard Nagar New Delhi India 110062

autor

Singh M.

• Hamdard University Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy Hamdard Nagar New Delhi India 110062

autor

Tamboli E. T

• Hamdard University Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy Hamdard Nagar New Delhi India 110062

autor

Parveen R

• Hamdard University Department of Pharmaceutics, Faculty of Pharmacy Hamdard Nagar New Delhi India 110062

autor

Ahmad S.

• Hamdard University Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy Hamdard Nagar New Delhi India 110062

Bibliografia

• [1] Anonymous, The Wealth of India, A Dictionary of Indian Raw Materials and Industrial Products, Publications & Information Directorate, CSIR, New Delhi, 1988, pp. 114–118
• [2] Anonymous, The Unani Pharmacopoeia of India, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 2007, pp. 32–33
• [3] A.K. Gupta, M. Sharma, and N. Tandon, Reviews on Indian Medicinal Plants, Indian Council of Medical Research, New Delhi, 2004, pp. 165–185
• [4] Anonymous, The Ayurvedic Pharmacopoeia of India, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 1999, pp. 34–35
• [5] Anonymous, National Formulary of Unani Medicine, Ministry of Health and Family Welfare, Department of AYUSH, Govt. of India, New Delhi, 2006, pp. 30–31
• [6] S. Letasiova, S. Jantova, M. Muckova, and M. Theiszova, Biomed. Pap. Med. Fac. Univ. Palacky Olomouc Czech Repub., 149, 461 (2005)
• [7] H. Rajaian, J. Jalaee, and A. Aghajani, Int. J. Poultry Sci., 5, 395 (2006)
• [8] M. Pitea, P. Petcu, T. Goina, and N. Preda, Planta Med., 21, 177 (1972)
• [9] W. Li and J.F. Fitzloff, J. Pharm. Pharmacol., 54, 435 (2002)
• [10] G. Srinivasan, K. Unnikrishnan, A. Rema Shree, and I. Balachandran, Indian J. Pharm. Sci., 70, 96 (2008)
• [11] C.M. Chen and H.C. Chang, J. Chromatogr. B, 665, 117 (1995)
• [12] A. Kong, X. Li, Z. Ni, and Y. Wang, Se Pu., 22, 89 (2004)
• [13] Y.R. Li, Y. Jiang, F. Hao, and X.Y. Ding, Zhongguo Zhong Yao Za Zhi, 32, 2597 (2007)
• [14] C. Ma, M. Fan, Y. Tang, Z. Li, Z. Sun, G. Ye, and C. Huang, Biomed. Chromatogr., 22, 835 (2008)
• [15] P.K. Gupta, M. Hubbard, B. Gurley, and H.P. Hendrickson, J. Pharm. Biomed. Anal., 49, 1021 (2009)
• [16 K.K. Rout, S. Pradhan, and S.K. Mishra, J. AOAC Int., 91, 1149 (2008)
• [17] International Conference on Harmonization (ICH) of Technical Requirements for the Registration of Pharmaceuticals for Human Use, Validation of Analytical Procedures: Methodology, Geneva, 1996
• [18] M.J. Ansari, S. Ahmad, K. Kohli, J. Ali, and R.K. Khar, J. Pharm. Biomed. Anal., 39, 132 (2005)
• [19] S. Ahmad, M. Rizwan, R. Parveen, M. Mujeeb, and M. Aquil, Chromatographia, 67, 441 (2008)
• [20] M.A. Bhat, S. Ahmad, A. Mujib, and M. Zafar, Chem. Pharm. Bull., 56, 17 (2008)
• [21] P. Alam, M. Ali, R. Singh, Madhurima, S. Ahmad, and F. Shakeel, J. Chromatogr. Sci., 47, 910 (2009)
• [22] S. Baboota, S. Faiyaz, A. Ahuja, J. Ali, S. Shafiq, and S. Ahmad, Acta Chromatogr., 18, 116 (2007)
• [23] N. Ahmad, J. Ali, S. Baboota, and S. Ahmad, Acta Chromatogr., 20, 277 (2008)
• [24] R. Parveen, S. Ahmad, S. Baboota, J. Ali, and A. Ahuja, Biomed. Chromatogr., 24, 639 (2010)