On the segmentation of the Comet Assay images

• Autorzy: Smolka B. , Szczepański M. , Świerniak A. , Palus H. , Plataniotis K. N.
• Języki publikacji: EN

Abstrakty

EN

The single cell gel electrophoresis, called Comet Assay is a microelectrophoretic technique of direct visualization of DNA damage at the cell level. In the comet assay, the cells suspended in an agarose gel on a microscope slide are subjected to lysis, unwinding of DNA and electrophoresis. After staining with fluorescent DNA binding dye, cells with DNA damage display increased migration of genetic material from the cell nucleus. Under the influence of weak, statics electric field, charged DNA migrates away from the nucleus forming a so called comet. The damage is quantified by measuring the amound of the genetic material, which migrates from the nucleus to form the comet tail. The foremost advantage of the comet assay is that it analyses individual cells, thus allowing the measurement of the heterogeneity of response within a cell population. In this paper we present three novel method of the comet tail and head extraction.

Słowa kluczowe

EN

single cell gel electrophoresis; SCGE; Comet Assay; noise reduction; image enhancement; segmentation; random walk; active contours; snakes

PL

elektroforeza żelowa; Comet Assay; redukcja szumu; wzmocnienie obrazu; segmentacja; błądzenie losowe; aktywne kontury

• Wydawca: University of Silesia, Institute of Informatics, Computer Systems Department
• Czasopismo: Journal of Medical Informatics & Technologies
• Rocznik: 2001
• Tom: Vol. 2, Part. 1
• Strony: MI29--MI39
• Opis fizyczny: Bibliogr. 22 poz., rys.

Twórcy

autor

Smolka B.

• Silesian University of Technology, Department of Automatic Control, Akademicka 16 Str, 44-101 Gliwice, Poland

autor

Szczepański M.

• Silesian University of Technology, Department of Automatic Control, Akademicka 16 Str, 44-101 Gliwice, Poland

autor

Świerniak A.

• Silesian University of Technology, Department of Automatic Control, Akademicka 16 Str, 44-101 Gliwice, Poland

autor

Palus H.

• Silesian University of Technology, Department of Automatic Control, Akademicka 16 Str, 44-101 Gliwice, Poland

autor

Plataniotis K. N.

• Edward S. Rogers Sr. Department of Electrical and Computer Engineering, University of Toronto,10 King's College Road, Toronto, Canada

Bibliografia

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