Kinetic properties of Lipase obtained from Pseudomonas aeruginosa isolated from crude oil contaminated soil

• Autorzy: Femi-Ola Titilayo Olufunke , Orjiakor Paul Ikechukwu , Enerijiofi Kingsley Erhons , Oke Iyanuoluwa Omolade , Fatoyinbo Adebayo Adeola

Identyfikatory

DOI

10.17402/363

• Języki publikacji: EN

Abstrakty

EN

Soil contaminated with crude oil has negatively affected some of the communities in Ekiti State region in Nigeria. There is an increased interest in microbial lipases because of their enormous potential in industrial and biotechnological applications. This study investigated the characteristics of partially-purified lipase from Pseudomonas aeruginosa isolated from oil-contaminated soil in Ado-Ekiti, Ekiti State. The lipase was purified by gel-filtration, having a molecular weight of 39.11 kDa, with Km and Vmax values of 12.50 and 28.86, respectively. The enzyme had an optimal pH of 8.0 and exhibited its maximal activity at 50°C, whereas the relatively stable temperature and pH were 40°C and 5.0, respectively. The enzyme activity was enhanced by olive oil, which served as the carbon source. Sodium chloride enhanced lipase activity, while calcium chloride acted as mild inhibitor, and iron chloride acted a strong inhibitor. The lipase from Pseudomonas aeruginosa possessed properties of an industrial enzyme and will be useful for biodegradation and bioremediation studies.

Słowa kluczowe

EN

biodegradation; enzymes; gel filtration; lipase; olive oil; Pseudomonas aeruginosa

• Wydawca: Wydawnictwo Naukowe Akademii Morskiej w Szczecinie
• Czasopismo: Zeszyty Naukowe Akademii Morskiej w Szczecinie
• Rocznik: 2019
• Tom: nr 59 (131)
• Strony: 154--161
• Opis fizyczny: Bibliogr. 38 poz., rys. tab.

Twórcy

autor

Femi-Ola Titilayo Olufunke

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria

autor

Orjiakor Paul Ikechukwu

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria

autor

Enerijiofi Kingsley Erhons

• Samuel Adegboyega University, College of Basic and Applied Sciences Department of Biological Sciences, Ogwa, Edo State, Nigeria

autor

Oke Iyanuoluwa Omolade

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria

autor

Fatoyinbo Adebayo Adeola

• Ekiti State University, Faculty of Science, Department of Microbiology Ado-Ekiti, Ekiti State, Nigeria

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).