Towards in-vivo assessment of fluorescence lifetime: imaging using time-gated intensified CCD camera

• Autorzy: Sawosz P. , Wojtkiewicz S. , Kacprzak M. , Zieminska E. , Morawiec M. , Maniewski R. , Liebert A.

Identyfikatory

DOI

10.1016/j.bbe.2018.08.006

• Języki publikacji: EN

Abstrakty

EN

A novel technique for imaging of a small animal with application of time-gated intensified CCD camera was proposed. The time-resolved method based on emission of picosecond light pulses and detection of the light penetrating in tissues was applied. In this technique, the fluorescence photons, excited in the dye circulating in the tissue, that diffusely penetrate in the optically turbid medium are detected. The data acquired during measurements carried out on a rat was analyzed in order to estimate fluorescence life time which depends strongly on the environment in which the dye is distributed. In the life time estimation a special emphasis was put on compensation of influence of the instrumental response function of the setup on the measured quantity. The proposed optical system was validated in series of phantom experiments, in which estimates of fluorescence lifetime of inclusions containing indocyanine green (ICG) were obtained. ICG is a dye revealing florescence properties in near-infrared wavelength region. Images of the estimate of fluorescence lifetime of the ICG accumulated in tissues of a rat were successfully acquired around six circular spots of illumination of the diameter of 6mm. Larger lifetime values were observed in lung/heart region of the animal. Aspect of sampling rate of the fluorescence lifetime images optimization was finally discussed.

Słowa kluczowe

EN

time-resolved imaging; fluorescence lifetime; small laboratory animal; time-gated CCD camera

PL

obrazowanie czasowo rozdzielcze; czas życia fluorescencji; małe zwierzę laboratoryjne; kamera CCD

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2018
• Tom: Vol. 38, no. 4
• Strony: 966--974
• Opis fizyczny: Bibliogr. 62 poz., rys., tab., wykr.

Twórcy

autor

Sawosz P.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, 4 Ks. Trojdena Str., 02-109 Warsaw, Poland

autor

Wojtkiewicz S.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland

autor

Kacprzak M.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland

autor

Zieminska E.

• Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Morawiec M.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland

autor

Maniewski R.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland

autor

Liebert A.

• Nalecz Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland

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