In vitro anti-adherence effect of probiotic Lactobacillus strains on human enteropathogens

• Autorzy: Nowak A. , Motyl I.
• Języki publikacji: EN

Abstrakty

EN

Probiotic bacteria possess great potential for producing antimicrobial substances that inhibit and control pathogenic bacteria in the human gastrointestinal tract. The aim of this study was to determine the anti-adherence properties of the probiotic Lactobacillus strains Lb. rhamnosus ŁOCK 0900, Lb. rhamnosus ŁOCK 0908, and Lb. casei ŁOCK 0919 (individually and in a 1:1:2 mixture) against the reference pathogens Clostridium difficile (ATCC 9689), Enterococcus faecalis (ATCC 29212), Listeria monocytogenes (ATCC 19115), and Staphylococcus aureus (ATCC 6538) using the Caco-2 human colon adenocarcinoma cell line. The mixture of probiotic strains inhibited the adherence of all pathogens, from 10.2% for E. faecalis ATCC 29212 to 97.2% for L. monocytogenes ATCC 19115. Of all the tested probiotic strains, Lb. casei ŁOCK 0919 reduced the adherence of S. aureus ATCC 6538 to the greatest extent (by 45.9%). These results suggest that adherence inhibition may involve competition for eukaryotic cell receptors and probiotic bacteria could protect the host cells from pathogen colonization and disease.

Słowa kluczowe

EN

adherence; intestinal microbiota; pathogens; inhibition; Caco-2 cells

PL

przyczepność; mikrobiota jelitowa; patogeny; inhibicja; komórki Caco-2

• Wydawca: Wydawnictwo Politechniki Łódzkiej
• Czasopismo: Biotechnology and Food Science
• Rocznik: 2017
• Tom: Vol. 81, nr 2
• Strony: 103--112
• Opis fizyczny: Bibliogr. 30 poz., wykr.

Twórcy

autor

Nowak A.

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, 90-924 Lodz

autor

Motyl I.

• Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, 90-924 Lodz

Bibliografia

• 1. Hill C, Guarner F, Reid G, Gibson GR, Marenstein DJ, Pot B, Morelli L, Canani RB, Flint J, Salminen S, Calder PC, Sanders ME The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Rev Gastroenterol Hepatol 2014, 11:506-514.
• 2. Reid G. Probiotics: definition, scope and mechanism of action. Best Practice Res Clin Gastroenterol 2016, 30:17-25.
• 3. Abedi D, Feizizadeh S, Akbari V, Jafarian-Dhkordi A. In vitro anti-bacterial and anti-adherence effects of Lactobacillus delbrueckii subsp. bulgaricus on Escherichia coli. Res Pharm Sci 2013, 8:260-268.
• 4. Li XJ, Yue LY, Guan XF, Qiao SY. The adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus. J Appl Microbiol 2007, 104:1082-1091.
• 5. Jankowska A, Laubitz D, Antushevich H, Zabielski R, Grzesiuk E. Competition of Lactobacillus paracasei with Salmonella enterica for adhesion to Caco-2 cells. J Biomed Biotechnol 2008, 357964.
• 6. Kankainen M, Paulin L, Tynkkynen S, von Ossowski I, Reunanen J, Partanen P, Satokari R, Vesterlund S, Hendrickx APAH, Lebeer S, De Keersmaecker SCJ, Vanderleyden J, Hämäläinen T, Laukkanen S, Salovuori N, Ritari J, Alatalo E, Korpela R, Mattila-Sandholm T, Lassig A, Hatakka K, Kinnunen KT, Karjalainen H, Saxelin M, Laakso K, Surakka A, Palva A, Auvinen TSP, de Vos WM. Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili containing a human-mucus binding protein. Proceed Nat Academy Sci USA 2009, 106:17193-17198.
• 7. Lim SM, Dong-Hyun A. Factors affecting adhesion of lactic acid bacteria to Caco-2 cells and inhibitory effect on infection of Salmonella Typhimurium. J Microbiol Biotechnol 2012, 22:1731-1739.
• 8. Velez MP, De Keersmaecker SCJ, Vanderleyeden J. Adherence factors of Lactobacillus in the human gastrointestinal tract. FEMS Microbiol Letters 2007, 276:140-148.
• 9. Cukrowska B, Motyl I, Kozakova H, Schwarzer M, Górecki RK, Klewicka E, Śliżewska K, Libudzisz Z. Probiotic Lactobacillus strains: in vitro and in vivo studies. Folia Microbiol 2009, 54(6):533-537.
• 10. Cukrowska B, Rosiak I, Klewicka E, Motyl I, Schwarzer M, Libudzisz Z, Kozakova H. Impact of heat-inactivated Lactobacillus casei and Lactobacillus paracasei strains on cytokine responses in whole blood cell cultures of children with atopic dermatitis. Folia Microbiol 2010, 55:277-280.
• 11. Aleksandrzak-Piekarczyk T, Koryszewska-Bagińska A, Bardowski J. Genome sequence of probiotic strain Lactobacillus rhamnosus (formerly Lactobacillus casei) LOCK900. Genome Announcements 2013, 1(4):1-2.
• 12. Koryszewska-Bagińska A, Aleksandrzak-Piekarczyk T, Bardowski J. Complete genome sequence of the probiotic strain Lactobacillus casei (formerly Lactobacillus paracasei) LOCK919. Genome Announcements 2013, 1(5):e00758-13.
• 13. Koryszewska-Bagińska A, Bardowski J, Aleksandrzak-Piekarczyk T. Genome sequence of probiotic strain Lactobacillus rhamnosus (formerly Lactobacillus casei) LOCK908. Genome Announcements 2014, 2(1):1-2.
• 14. Erdenlig S, Ainsworth AJ, Austin FW. Pathogenicity and production of virulence factors by Listeria monocytogenes isolates from channel catfish. J Food Protect 2000, 63(5):613-619.
• 15. Miura M, Kato H, Matsushita O. Identification of a novel virulence factor in Clostridium difficile that modulates toxin sensitivity of cultured epithelial cells. Infect Immunol 2011, 79(9):3810-3820.
• 16. Kim EB, Kopit LM, Harris LJ, Marco ML. Draft genome sequence of the quality control strain Enterococcus faecalis ATCC 29212. J Bacteriol 2012, 194:6006-6007.
• 17. Nowak A, Czyżowska A, Stańczyk M. Protective activity of probiotic bacteria against 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) and 2-amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine (PhIP) – An in vitro study. Food Add Contam Part A 2015, 32(11):1927-1938.
• 18. Nowak A, Motyl I, Śliżewska K, Libudzisz Z, Klewicka E. Adherence of probiotic bacteria to human colon epithelial cells and inhibitory effect against enteric pathogens. Int J Dairy Technol 2016, 69:532-539.
• 19. Aleksandrzak-Piekarczyk T, Koryszewska-Bagińska A, Grynberg M, Nowak A, Cukrowska B, Kozakova H, Bardowski J. Genomic and functional characterization of the unusual pLOCK 0919 plasmid harboring the spaCBAPili cluster in Lactobacillus casei LOCK 0919. Genome Biol Evol 2015, 8(1):202-217.
• 20. Gawlik D, Slickers P, Engelmann I, Muller E, Luck C, Friedrichs A, Ehricht R, Monecke S. DNA-Microarray-based genotyping of Clostridium difficile. BMC Microbiol 2015, 15:158.
• 21. Banerjee P, Merkel GJ, Bhunia AK. Lactobacillus delbrueckii ssp. Bulgaricus B-30892 can inhibit cytotoxic effects and adhesion of pathogenic Clostridium difficile to Caco-2 cells. Gut Pathogens 2009, 1(1):8.
• 22. Gueimonde M, Jalonen L, Hec F, Hiramatsu M, Salminen S. Adhesion and competitive inhibition and displacement of human enteropathogens by selected lactobacilli. Food Res Int 2006, 39:467-471.
• 23. Valreads MMC, Van Der Mei HC, Reid G, Busscher HJ. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl Environ Microbiol 1996, 62:1958-1963.
• 24. Corr SC, Gahan CG, Hill C. Impact of selected Lactobacillus and Bifidobacterium species on Listeria monocytogenes infection and the mucosal immune response. FEMS Immunol Med Microbiol 2007, 50:380-388.
• 25. Cruz CD, Silvestre FA, Kinoshita EM, Landgraf M, Franco BD, Destro MT. Epidemiological survey of Listeria monocytogenes in a gravlax salmon processing line. Braz J Microbiol 2008, 39:375-383.
• 26. Bendali F, Hebraud M, Sadoun D. Anti-bacterial and anti-adherence activities of a probiotic strain of Lactobacillus paracasei against Listeria monocytogenes. International J Appl Microbiol Biotechnol Res 2014, 2:52-63.
• 27. Ren D, Li C, Qin Y, Yin R, Li X, Tian M, Du S, Guo H, Liu C, Zhu N, Sun D, Li Y, Jin N. Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment. Anaerobe 2012, 18:508-515.
• 28. Ray AJ, Pultz NJ, Bhalla A, Aron DC, Donskey CJ. Coexistence of vancomycinresistant enterococci and Staphylococcus aureus in the intestinal tracts of hospitalised patients. Clin Inf Dis 2003, 37:875-881.
• 29. Suh L, Coffin S, Leckerman KH, Gelfand JM, Honig PJ, Yan AC. Methicillinresistant Staphylococcus aureus colonization in children with atopic dermatitis. Pediatric Dermatol 2008, 5:528-534.
• 30. Tuomola EM, Ouwehand AC, Salminen SJ. The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus. FEMS Immunol Medical Microbiol 1999, 26:137-142.

Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).