Fluctuations in settling velocity of red blood cell aggregates

• Autorzy: Kempczyński A , Bosek M. , Grzegorzewski B.

Identyfikatory

DOI

10.5277/oa140302

• Języki publikacji: EN

Abstrakty

EN

Sedimentation of red blood cell aggregates was experimentally investigated by optical imaging. Suspensions of red blood cell at low hematocrit were obtained from blood of healthy donors. The velocity of three-dimensional red blood cell aggregates was measured using particle image velocimetry. The magnitude and spatial correlation functions of the velocity fluctuations of the settling aggregates were determined. It is shown that the fluctuations in the settling velocity exhibit characteristic correlations in the form of swirls. The formation of 3-D red blood cell aggregates leads to a large initial swirl. The growth of the aggregates and their sedimentation diminishes the swirls size.

Słowa kluczowe

EN

particle image velocimetry; red blood cell aggregation; sedimentation; velocity fluctuations

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Optica Applicata
• Rocznik: 2014
• Tom: Vol. 44, nr 3
• Strony: 365--373
• Opis fizyczny: Bibliogr. 28 poz., rys., wykr.

Twórcy

autor

Kempczyński A

• Biophysics Department, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 13, 85-067 Bydgoszcz, Poland

autor

Bosek M.

• Biophysics Department, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 13, 85-067 Bydgoszcz, Poland

autor

Grzegorzewski B.

• Biophysics Department, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, ul. Jagiellońska 13, 85-067 Bydgoszcz, Poland

Bibliografia

• [1] BATCHELOR G.K., Sedimentation in a dilute dispersion of spheres, Journal of Fluid Mechanics 52(2), 1972, pp. 245–268.
• [2] CAFLISCH R.E., LUKE J.H.C., Variance in the sedimentation speed of a suspension, Physics of Fluids 28(3), 1985, pp. 759–760. 372 A. KEMPCZYŃSKI et al.
• [3] NICOLAI H., GUAZZELLI E., Effect of the vessel size on the hydrodynamic diffusion of sedimenting spheres, Physics of Fluids 7(1), 1995, pp. 3–5.
• [4] SEGRÈ P.N., HERBOLZHEIMER E., CHAIKIN P.M., Long-range correlations in sedimentation, Physical Review Letters 79(13), 1997, pp. 2574–2577.
• [5] SAINTILLAN D., SHAQFEH E.S.G., DARVE E., The growth of concentration fluctuations in dilute dispersions of orientable and deformable particles under sedimentation, Journal of Fluid Mechanics 553, 2006, pp. 347–388.
• [6] SEGRÈ P.N., LIU F., UMBANHOWAR P., WEITZ D.A., An effective gravitational temperature for sedimentation, Nature 409(6820), 2001, pp. 594–597.
• [7] SNABRE P., POULIGNY B., METAYER C., NADAL F., Size segregation and particle velocity fluctuations in settling concentrated suspensions, Rheologica Acta 48(8), 2009, pp. 855–870.
• [8] ARMSTRONG J.K., WENBY R.B., MEISELMAN H.J., FISHER T.C., The hydrodynamic radii of macromolecules and their effect on red blood cell aggregation, Biophysical Journal 87(6), 2004, pp. 4259–4270.
• [9] FABRY T.L., Mechanism of erythrocyte aggregation and sedimentation, Blood 70(5), 1987, pp. 1572–1576.
• [10] MUTRYNOWSKA J., GRZEGORZEWSKI B., Optical analysis of red blood cell sediment formation, Biorheology 44(4), 2007, pp. 285–297.
• [11] REUBEN A.J., SHANNON A.G., Some problems in the mathematical modelling of erythrocyte sedimentation, Mathematical Medicine and Biology 7(3), 1990, pp. 145–156.
• [12] PRIBUSH A., MEYERSTEIN N., Methodological aspects of erythrocyte aggregation, Recent Patents on Anti-Cancer Drug Discovery 2(3), 2007, pp. 240–245.
• [13] PRIBUSH A., MEYERSTEIN D., MEYERSTEIN N., The mechanism of erythrocyte sedimentation. Part 2: The global collapse of settling erythrocyte network, Colloids and Surfaces B: Biointerfaces 75(1), 2010, pp. 224–229.
• [14] PONDER E., On sedimentation and rouleaux formation–II, Experimental Physiology 16(2), 1926, pp. 173–194.
• [15] KERNICK D., JAY A.W.L., ROWLANDS S., SKIBO L., Experiments on rouleau formation, Canadian Journal of Physiology and Pharmacology 51(9), 1973, pp. 690–699.
• [16] SHIGA T., IMAIZUMI K., HARADA N., SEKIYA M., Kinetics of rouleaux formation using TV image analyzer. I. Human erythrocytes, American Journal of Physiology – Heart and Circulatory Physiology 245(2), 1983 pp. H252–H258.
• [17] BARSHTEIN G., WAJNBLUM D., YEDGAR S., Kinetics of linear rouleaux formation studied by visual monitoring of red cell dynamic organization, Biophysical Journal 78(5), 2000, pp. 2470–2474.
• [18] SAMSEL R.W., PERELSON A.S., Kinetics of rouleau formation. I. A mass action approach with geometric features, Biophysical Journal 37(2), 1982, pp. 493–514.
• [19] POP C.V.L., NEAMTU S., Aggregation of red blood cells in suspension: study by light-scattering technique at small angle, Journal of Biomedical Optics 13(4), 2008, article 041308.
• [20] TSINOPOULOS S.V., SELLOUNTOS E.J., POLYZOS D., Light scattering by aggregated red blood cells, Applied Optics 41(7), 2002, pp. 1408–1417.
• [21] SHVARTSMAN L.D., FINE I., Optical transmission of blood: effect of erythrocyte aggregation, IEEE Transactions on Biomedical Engineering 50(8), 2003, pp. 1026–1033.
• [22] BEKSHAEV A.YA., ANGELSKY O.V., HANSON S.G., ZENKOVA C.YU., Scattering of inhomogeneous circularly polarized optical field and mechanical manifestation of the internal energy flows, Physical Review A 86, 2012, article 023847.
• [23] SHEPPARD C.J.R., Fractal model of light scattering in biological tissue and cells, Optics Letters 32(2), 2007, pp. 142–144.
• [24] MIN XU, Electric field Monte Carlo simulation of polarized light propagation in turbid media, Optics Express 12(26), 2004, pp. 6530–6539. Fluctuations in settling velocity of red blood cell aggregates 373
• [25] KEMPCZYŃSKI A., BOSEK M., GRZEGORZEWSKI B., Comparison of Hough and Fourier transform approach in the study of kinetics of red blood cell aggregates, Proceedings of SPIE 7141, 2008, article 714118.
• [26] KEMPCZYŃSKI A., GRZEGORZEWSKI B., Estimation of red blood cell aggregate velocity during sedimentation using the Hough transform, Optics Communications 281(21), 2008, pp. 5487–5491.
• [27] KALIVIOTIS E., DUSTING J., BALABANI S., Spatial variation of blood viscosity: modelling using shear fields measured by a μPIV based technique, Medical Engineering and Physics 33(7), 2011, pp. 824–831.
• [28] BERGOUGNOUX L., GHICINI S., GUAZZELLI E., HINCH J., Spreading fronts and fluctuations in sedimentation, Physics of Fluids 15(7), 2003, pp. 1875–1887.