Stability-indicating RP-HPLC assay for simultaneous determination of chlorpheniramine maleate and prednisolone in veterinary injection

• Autorzy: Ali Amir , Farooq Umar , Ahmed Mahmood , Athar Muhammad Makshoof , Salman M. , Arif Saira , Nadeem Kashif , Naz Hassan

Identyfikatory

DOI

10.1556/1326.2019.00593

• Języki publikacji: EN

Abstrakty

EN

The present work described a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of chlorpheniramine maleate (CHRM) and prednisolone acetate (PRED) in injection samples by high-performance liquid chromatography (HPLC) coupled with UV–Vis detection. Chromatographic separation was accomplished, employing isocratic mode and a mobile phase comprised of acetonitrile and a phosphate buffer (50:50, v/v, 30 °C), adjusted to pH 3.0. The flow rate used was 1.0 mL/min on a Thermo Hypersil ODS C18 column (5 μm, 4.6 × 250 mm), and the injection volume of sample was 20 μL. Analysis of CHRM and PRED was performed at a wavelength of 254 nm. The runtime for analysis was 12.5 min, and the retention times of CHRM and PRED were found to be 2.81 and 5.07 min, respectively. The calibration graph showed linearity over the concentration range 10–70 μg/mL for CHRM and 20–140 μg/mL for PRED with a coefficient of determination (R2) ≥0.9986. Repeatability and reproducibility (expressed as % RSD) were lower than 1.72 and 1.47%, respectively. The proposed HPLC method was demonstrated to be simple and rapid for the determination of CHRM and PRED in injection formulation, providing recoveries between 101.6–102.3%, whereas complete separation of degradation products, from analyte under investigation, provided the specificity of the proposed HPLC method.

Słowa kluczowe

EN

antihistamines; corticosteroid; HPLC; validation; stability-indicating method

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2020
• Tom: Vol. 32, no. 2
• Strony: 122--127
• Opis fizyczny: Bibliogr. 26 poz., rys.

Twórcy

autor

Ali Amir

• University of the Punjab, Lahore, 54590, Pakistan

autor

Farooq Umar

• University of the Punjab, Lahore, 54590, Pakistan

autor

Ahmed Mahmood

• Renacon Pharma Limited, Lahore, 54600, Pakistan

autor

Athar Muhammad Makshoof

• University of the Punjab, Lahore, 54590, Pakistan

autor

Salman M.

• University of the Punjab, Lahore, 54590, Pakistan

autor

Arif Saira

• University of the Punjab, Lahore, 54590, Pakistan

autor

Nadeem Kashif

• Schazoo Pharmaceutical Laboratories, Lahore-Jaranwala Road, Sheikhupura, Pakistan

autor

Naz Hassan

• Schazoo Pharmaceutical Laboratories, Lahore-Jaranwala Road, Sheikhupura, Pakistan

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Uwagi

PL

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).