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Warianty tytułu
Języki publikacji
Abstrakty
In this study, different columns, including C18, C8, and CN, were investigated for the analysis of cyclotides in Viola ignobilis. Because of the complexity of the cyclotide matrix in V. ignobilis and the presence of their different isomers, suitable separations could not be obtained by high-performance liquid chromatography (HPLC). Also, a miniaturized column with a powerful determination technique, such as nanoliquid chromatography-Fourier transform mass spectroscopy (nano-LC-FTMS), could not succeed in the identification of all the cyclotides. Thus, a multidimensional liquid chromatography (MDLC), in the heart-cutting and mixed-bed modes, has been developed to separate the cyclotides. In the heart-cutting MDLC, the C8-C18 and C8-CN conditions were investigated and a good resolution could be obtained for the isolation of a few specific peaks. In the mixed-bed mode, a C8-CN condition was investigated and good separation was obtained by a gradient condition. The results of these methods are compared.
Czasopismo
Rocznik
Tom
Strony
641--651
Opis fizyczny
Bibliogr. 15 poz., rys., tab.
Twórcy
autor
- Shahid Beheshti University Medicinal Plants and Drugs Research Institute Evin, Tehran Iran
autor
- Shahid Beheshti University Medicinal Plants and Drugs Research Institute Evin, Tehran Iran
autor
- Shahid Beheshti University Medicinal Plants and Drugs Research Institute Evin, Tehran Iran
autor
- Chemistry and Chemical Engineering Research Center Tehran P.O. Box 14335-186 Iran
Bibliografia
- [1] D.J. Craik, N.L. Daly, T. Bond, and C. Waine, J. Mol. Biol, 294, 1327 (1999)
- [2] N.L. Daly, K.R. Gustafson, and D.J. Craik, FEBS Lett., 574, 69 (2004)
- [3] K.R. Gustafson, T.C. Mckee, and H.R. Bokesch, Curr. Protein Pept. Sci., 5, 331 (2004)
- [4] A. Koltay, N.L. Daly, K.R. Gustafson, and D.J. Craik, Int. J. Pept. Res. Ther., 11, 99 (2005)
- [5] S. Gunasekera, N.L. Daly, M.A. Anderson, and D.J. Craik, IUBMB Life, 58, 515 (2006)
- [6] Saska, M.L. Colgrave, A. Jones, M.A. Anderson, and D.J. Craik, J. Chromatogr. B, 872, 107 (2008)
- [7] B.L. Barbeta, A.T. Marshall, A.D. Gillon, D.J. Craik, and M.A. Anderson, PNAS, 105, 1221 (2008)
- [8] S.P. Dixon, I.D. Pitfield, and D. Perrett, Biomed. Chromatogr., 20, 508 (2006)
- [9] R.A. Shellie and P.R. Haddad, Anal. Bioanal. Chem. 386, 405 (2006)
- [10] K. Okusa, H. Tanaka, and M. Ohira, J. Chromatogr. A, 869, 143 (2000)
- [11] J. Lv, H. Liang, Q. Yuan, Y. Xu, and T. Wang, Separ. Sci. Tech., 45, 1104 (2010)
- [12] A. Ghassempour, M. Noruzi, M. Zandehzaban, Z. Talebpour, A.Y. Khosroshahi, N.M. Najafi, M. Valizadeh, T. Poursaberi, H. Hekmati, and H. Naghdibadi, H.Y. Aboul-Enein, J. Liq. Chromatogr. Rel. Tech., 31, 382 (2008)
- [13] Y. Shen, R. Zhao, S.J. Berger, G.A. Anderson, N. Rodriguez, and R.D. Smith, Anal. Chem. 74, 4235 (2002)
- [14] Y. Ishihama, J. Chromatogr. A, 1067, 73 (2005)
- [15] X. Feng and M.M. Siegel, Anal. Bioanal. Chem, 389, 1341 (2007)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-4293563c-94e0-41a0-b545-4ad64575930e