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Microbial communities and relationship with biofilm spatial distributions in subsurface wastewater infiltration systems

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Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
A pilot-scale subsurface wastewater infiltration system (SWIS) was designed for the treatment of polluted river water. The components of microbial communities have been identified and characterized and their dependences on some indicators of biofilm formation in the SWIS have been determined. The average efficiencies of COD, TN and removal were 43.3%, 28.8% and 79.6%, respectively. According to the denaturing gradient gel electrophoresis (DGGE) profile, high intensity and uniform bands were generated, indicating an abundant microbial community in each layer of the SWIS. Furthermore, the Shannon index analysis showed high correlation to the spatial distribution of microbial communities as well as the quantity of biofilm in each sample, which were characterized by measuring volatile suspended solids (VSS), phospholipids, proteins and polysaccharides. Sequencing of partial 16S rRNA gene fragments revealed that the composition of the total bacterial communities was dominated by Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Pseudomonas, Bacillus, Flavobacteriaceae, TM-7, and other uncultured bacteria. These bacteria may contribute to nutrient removal in SWIS. +4 NH –N
Rocznik
Strony
55--69
Opis fizyczny
Bibliogr. 25 poz., tab., rys.
Twórcy
autor
  • College of Architecture and Environment, Sichuan University, Chengdu 610065, China.
  • School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou 450001, China
  • College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
autor
  • College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Bibliografia
  • [1] HE S.B., YAN L., KONG H.N., LIU Z.M., WU E.Y., HU Z.B., Treatment efficiencies of constructed wet-lands for eutrophic landscape river water, Pedosphere, 2007, 17 (4), 522.
  • [2] YANG J., ZHANG L.B., WU Y.F., WANG Y.Y., LI C., LIU W., Treatment and hydraulic performances of the NiiMi process for landscape water, J. Zhejiang Univ.-Sci. A, (Appl. Phys. Eng.), 2010, 11 (2), 132.
  • [3] ZHANG L.B., XING M.Y., WU Y.F., HUANG Z.D., YANG J., Spatial distributions of biofilm properties and flow pattern in NiiMi process, Bioresource. Technol., 2011, 102, 1406.
  • [4] AVINASH M.K., PRAVIN D.N., OZA G.H., SHANKAR H.S., Treatment of municipal wastewater using laterite-based constructed soil filter, Ecol. Eng., 2009, 35, 1051.
  • [5] ZOU J.L., DAI Y., SUN T.H., LI Y.H., LI G.B., LI Q.Y., Effect of amended soil and hydraulic load on enhanced biological nitrogen removal in labscale SWIS, J. Hazard. Mater., 2009, 163, 816.
  • [6] MUYZER G., DE WAAL E.C., UITTERLINDEN A.G., Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA, Appl. Environ. Microbiol., 1993, 59, 695.
  • [7] WAKELIN S.A., COLLOFF M.J., KOOKANA R.S., Effect of wastewater treatment plant effluent on microbial function and community structure in the sediment of a freshwater stream with variable seasonal flow, Appl. Environ. Microbiol., 2008, 74, 2659.
  • [8] MULLING B.T.M., SOETER A.M., VAN DER GEEST H.G., ADMIRAAL W., Changes in the planktonic microbial community during residence in a surface flow constructed wetland used for tertiary wastewater treatment, Sci. Total. Environ., 2014, 466–467, 881.
  • [9] LIU G.H., YE Z.F., LI H.Y., CHE R., CUI L.L., Biological treatment of hexanitrostilbene (HNS) produced wastewater using an anaerobic–aerobic immobilized microbial system, Chem. Eng. J., 2012, 213, 118.
  • [10] MINA I.A.P., COSTA M., MATOS A., CALHEIROS C., CASTRO P.M.L., Polishing domestic wastewater on a subsurface flow constructed wetland: organic matter removal and microbial monitoring, Int. J. Phytoremediat., 2011, 13 (10), 947.
  • [11] WANG L.M., GUO F.H., ZHENG Z., LUO X.Z., ZHANG J.B., Enhancement of rural domestic sewage treatment performance, and assessment of microbial community diversity and structure using tower vermifiltration, Bioresource Technol., 2011, 102 (20), 9462.
  • [12] SAMBROOK J., FRISCH E.F., MANIATIS T., Molecular Cloning. Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, New York 1989.
  • [13] BOSSHARD P.P., SANTINI Y., GRUTER D., STETTLER R., BACHOFEN R., Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis, FEMS Microbiol. Ecol., 2000, 31 (2), 173.
  • [14] SHANNON A.E., WEAVER W., The Mathematical Theory of Communication, University Illinois Press, Urbana, IL, 1963.
  • [15] FINDLAY R.H., KING G.M., WATLING L., Efficacy of phospholipid analysis in determining microbial biomass in sediments, Appl. Environ. Microbiol., 1989, 55, 2888.
  • [16] ZHANG X.Q., BISHOP P.L., Biodegradability of biofilm extracellular polymeric substances, Chemo-sphere, 2003, 50, 63.
  • [17] LIU H., FANG H.H.P., Extraction of extracellular polymeric substances (EPS) of sludges, J. Biotechnol., 2002, 95, 249.
  • [18] ONG S.A., UCHIYAMA K., INADAMA D., ISHIDA Y., YAMAGIWA K., Performance evaluation of laboratory scale up-flow constructed wetlands with different designs and emergent plants, Bioresource Technol., 2010, 101, 7239.
  • [19] SUTHERSAN S.S., Natural and Enhanced Remediation Systems, Acradis, Lewis Publisher, Washington, DC, 2002.
  • [20] ACHAK M., MANDI L., OUAZZANI N., Removal of organic pollutants and nutrients from olive mill wastewater by a sand filter, J. Environ. Manage., 2009, 90 (8), 2771.
  • [21] ARROYO P., ANSOLA G., SÁENZ DE MIERA L.E., Effects of substrate, vegetation and flow on arsenic and zinc removal efficiency and microbial diversity in constructed wetlands, Ecol. Eng., 2013, 51, 95.
  • [22] AGUILAR J.R.M., CABRIALES J.J.P., VEGA M.M., Identification and characterization of sulfur-oxidizing bacteria in an artificial wetland that treats wastewater from a tannery, Int. J. Phytoremediat., 2008, 10, 359.
  • [23] BEG Q.K., SAXENA R.K., GUPTA R., De-repression and subsequent induction of protease synthesis by Bacillus mojavensis under fed-batch operations, Process. Biochem., 2002, 37, 1103.
  • [24] VYMAZAL J., Removal of nutrients in various types of constructed wetlands, Sci. Total. Environ., 2007, 380, 48.
  • [25] GRANT W.D., LONG P.E., Environmental microbiology, Blackien and Son, Glasgow 1981.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-42757cdb-53ee-4d87-aa04-cdf6c6f22b6a
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